MRGX3 (MRGPRX3) (NM_054031) Human Tagged ORF Clone

CAT#: RG209610

  • TrueORF®

MRGPRX3 (tGFP-tagged) - Human MAS-related GPR, member X3 (MRGPRX3)

ORF Plasmid: DDK tGFP



  "NM_054031" in other vectors (4)

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CNY 4,000.00

CNY 4,370.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (5)
pCMV6-AC-GFP, mammalian vector with C-terminal tGFP tag, 10ug
    • 10 ug

CNY 6,080.00


Mouse monoclonal turboGFP antibody, clone OTI2H8
    • 100 ul

CNY 1,000.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


Rabbit polyclonal anti-MRGX3 antibody
    • 100 ul

CNY 1,999.00
CNY 3,280.00

Specifications

Product Data
Type Human Tagged ORF Clone
Tag TurboGFP
Synonyms GPCR; MRGX3; SNSR1; SNSR2
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RG209610 representing NM_054031
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGATTCAACCATCCCAGTCTTGGGTACAGAACTGACACCAATCAACGGACGTGAGGAGACTCCTTGCT
ACAAGCAGACCCTGAGCTTCACGGGGCTGACGTGCATCGTTTCCCTTGTCGCGCTGACAGGAAACGCGGT
TGTGCTCTGGCTCCTGGGCTGCCGCATGCGCAGGAACGCTGTCTCCATCTACATCCTCAACCTGGTCGCG
GCCGACTTCCTCTTCCTTAGCGGCCACATTATACGTTCGCCGTTACGCCTCATCAATATCCGCCATCCCA
TCTCCAAAATCCTCAGTCCTGTGATGACCTTTCCCTACTTTATAGGCCTAAGCATGCTGAGCGCCATCAG
CACCGAGCGCTGCCTGTCCATCCTGTGGCCCATCTGGTACCACTGCCGCCGCCCCAGATACCTGTCATCG
GTCATGTGTGTCCTGCTCTGGGCCCTGTCCCTGCTGCGGAGTATCCTGGAGTGGATGTTCTGTGACTTCC
TGTTTAGTGGTGCTGATTCTGTTTGGTGTGAAACGTCAGATTTCATTACAATCGCGTGGCTGGTTTTTTT
ATGTGTGGTTCTCTGTGGGTCCAGCCTGGTCCTGCTGGTCAGGATTCTCTGTGGATCCCGGAAGATGCCG
CTGACCAGGCTGTACGTGACCATCCTCCTCACAGTGCTGGTCTTCCTCCTCTGTGGCCTGCCCTTTGGCA
TTCAGTGGGCCCTGTTTTCCAGGATCCACCTGGATTGGAAAGTCTTATTTTGTCATGTGCATCTAGTTTC
CATTTTCCTGTCCGCTCTTAACAGCAGTGCCAACCCCATCATTTACTTCTTCGTGGGCTCCTTTAGGCAG
CGTCAAAATAGGCAGAACCTGAAGCTGGTTCTCCAGAGGGCTCTGCAGGACACGCCTGAGGTGGATGAAG
GTGGAGGGCAGCTTCCTCAGGAAACCCTGGAGCTGTCGGGAAGCAGATTGGAGCAG


ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
>RG209610 representing NM_054031
Red=Cloning site Green=Tags(s)

MDSTIPVLGTELTPINGREETPCYKQTLSFTGLTCIVSLVALTGNAVVLWLLGCRMRRNAVSIYILNLVA
ADFLFLSGHIIRSPLRLINIRHPISKILSPVMTFPYFIGLSMLSAISTERCLSILWPIWYHCRRPRYLSS
VMCVLLWALSLLRSILEWMFCDFLFSGADSVWCETSDFITIAWLVFLCVVLCGSSLVLLVRILCGSRKMP
LTRLYVTILLTVLVFLLCGLPFGIQWALFSRIHLDWKVLFCHVHLVSIFLSALNSSANPIIYFFVGSFRQ
RQNRQNLKLVLQRALQDTPEVDEGGGQLPQETLELSGSRLEQ

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_054031
ORF Size 966 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_054031.2, NP_473372.2
RefSeq Size 1697 bp
RefSeq ORF 969 bp
Locus ID 117195
UniProt ID Q96LB0
Protein Families Druggable Genome, GPCR, Transmembrane
Gene Summary This gene encodes a member of the mas-related/sensory neuron specific subfamily of G protein coupled receptors. The encoded protein may be involved in sensory neuron regulation and in the modulation of pain. [provided by RefSeq, Oct 2009]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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