AER61 (EOGT) (NM_173654) Human Tagged ORF Clone
CAT#: RG209333
- TrueORF®
EOGT (tGFP-tagged) - Human chromosome 3 open reading frame 64 (C3orf64)
ORF Plasmid: DDK
"NM_173654" in other vectors (4)
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CNY 5,256.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | TurboGFP |
Synonyms | AER61; AOS4; C3orf64; EOGT1 |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RG209333 representing NM_173654
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTTAATGTTGTTTGTCTTTGGAGTCTTACTTCATGAAGTCTCACTGAGTGGTCAGAATGAAGCTCCTC CTAATACTCACAGCATTCCAGGCGAACCTCTGTATAACTATGCCAGCATCCGCTTGCCAGAGGAGCACAT TCCCTTCTTTTTGCACAACAATAGGCATATTGCCACTGTCTGTAGGAAAGACTCTCTTTGTCCATATAAG AAACACCTAGAGAAGCTAAAGTACTGCTGGGGTTATGAGAAATCCTGCAAACCAGAGTTCAGGTTTGGTT ACCCAGTTTGCAGCTATGTCGACATGGGATGGACGGACACTCTTGAGTCAGCTGAGGACATATTTTGGAA ACAAGCTGACTTTGGATATGCCAGAGAGAGGCTGGAGGAGATGCATGTGCTCTGTCAGCCTAAGGAAACG AGTGACTCAAGTCTGGTGTGTTCCCGTTATCTTCAGTACTGCAGGGCAACCAATCTCTATCTTGATTTAA GAAACATCAAGAGAAATCATGACAGATTTAAGGAGGACTTTTTCCAGAGTGGTGAAATTGGAGGGCACTG TAAACTTGACATCCGTACATTGACGTCTGAAGGTCAGCGCAAAAGCCCTCTGCAGTCATGGTTTGCTGAG CTACAAAGCTATACTCAGCTCAACTTCAGACCTATAGAAGATGCTAAATGTGACATTGTCATTGAAAAAC CAACATATTTCATGAAATTAGATGCAGGTGTTAACATGTATCACCACTTCTGTGATTTCATCAATCTTTA TATTACTCAGCACGTTAATAACTCATTCAGTACTGACGTGTACATCGTGATGTGGGACACCGATGGAAAA ATTCGAGTCACCATTCTTGCACGGAGCACAGAATACCGGAAAATCCTTAACCAAAATGAGCTTGTAAATG CACTGAAAACAGTATCTACATTTGAAGTCCAGATTGTTGATTACAAGTATAGAGAACTTGGGTTTTTAGA TCAACTAAGGATCACACACAACACGGACATATTTATTGGAATGCATGGAGCTGGTCTGACCCATTTACTT TTCCTTCCAGACTGGGCTGCTGTATTTGAACTGTACAACTGTGAAGATGAACGCTGTTACTTAGACTTGG CCAGGCTGAGAGGCGTTCACTACATCACTTGGCGACGGCAGAACAAAGTCTTTCCTCAGGATAAGGGCCA CCATCCAACCCTGGGGGAGCACCCGAAGTTCACCAACTACTCTTTCGATGTAGAAGAATTTATGTATCTT GTCCTTCAGGCTGCAGACCACGTATTGCAACACCCAAAGTGGCCATTTAAGAAGAAACATGATGAGCTA ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG209333 representing NM_173654
Red=Cloning site Green=Tags(s) MLMLFVFGVLLHEVSLSGQNEAPPNTHSIPGEPLYNYASIRLPEEHIPFFLHNNRHIATVCRKDSLCPYK KHLEKLKYCWGYEKSCKPEFRFGYPVCSYVDMGWTDTLESAEDIFWKQADFGYARERLEEMHVLCQPKET SDSSLVCSRYLQYCRATNLYLDLRNIKRNHDRFKEDFFQSGEIGGHCKLDIRTLTSEGQRKSPLQSWFAE LQSYTQLNFRPIEDAKCDIVIEKPTYFMKLDAGVNMYHHFCDFINLYITQHVNNSFSTDVYIVMWDTDGK IRVTILARSTEYRKILNQNELVNALKTVSTFEVQIVDYKYRELGFLDQLRITHNTDIFIGMHGAGLTHLL FLPDWAAVFELYNCEDERCYLDLARLRGVHYITWRRQNKVFPQDKGHHPTLGEHPKFTNYSFDVEEFMYL VLQAADHVLQHPKWPFKKKHDEL TRTRPLE - GFP Tag - V |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_173654 |
ORF Size | 1329 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_173654.1, NP_775925.1 |
RefSeq Size | 4152 bp |
RefSeq ORF | 1332 bp |
Locus ID | 285203 |
UniProt ID | Q5NDL2 |
Gene Summary | This gene encodes an enzyme that acts in the lumen of the endoplasmic reticulum to catalyze the transfer of N-acetylglucosamine to serine or threonine residues of extracellular-targeted proteins. This enzyme modifies proteins containing eukaryotic growth factor (EGF)-like domains, including the Notch receptor, thereby regulating developmental signalling. Mutations in this gene have been observed in individuals with Adams-Oliver syndrome 4. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2015] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC209333 | EOGT (Myc-DDK-tagged)-Human chromosome 3 open reading frame 64 (C3orf64) |
CNY 3,656.00 |
|
RC209333L3 | Lenti ORF clone of Human chromosome 3 open reading frame 64 (C3orf64), Myc-DDK-tagged |
CNY 5,890.00 |
|
RC209333L4 | Lenti ORF clone of Human chromosome 3 open reading frame 64 (C3orf64), mGFP tagged |
CNY 5,890.00 |
|
SC121381 | EOGT (untagged)-Human chromosome 3 open reading frame 64 (C3orf64) |
CNY 3,656.00 |