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BCDIN3D (NM_181708) Human Tagged ORF Clone
CAT＃: RG208818
- TrueORF®
BCDIN3D (tGFP-tagged) - Human BCDIN3 domain containing (BCDIN3D)
ORF Plasmid: DDK
"NM_181708" in other vectors (6)
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CNY 4,000.00
CNY 4,370.00
Product images
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG208818 representing NM_181708
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGGTGCCCACGGAACTGGATGGAGGGAGTGTTAAGGAGACCGCAGCGGAAGAGGAATCGCGAGTTC TGGCACCTGGCGCCGCCCCGTTCGGAAATTTTCCTCATTATTCTCGCTTCCACCCTCCGGAGCAACGGCT CCGCCTCCTGCCCCCGGAGCTGCTTCGACAGCTCTTTCCTGAGAGTCCCGAGAACGGGCCGATTCTGGGG CTCGACGTGGGGTGTAACTCCGGGGATCTGAGTGTGGCTCTATACAAACACTTCCTCTCCCTACCTGACG GGGAAACCTGCTCAGATGCCTCAAGAGAATTCCGTCTCCTCTGCTGCGACATAGATCCAGTCCTGGTGAA GCGAGCCGAAAAAGAATGTCCTTTTCCTGATGCCTTGACTTTTATCACCCTGGACTTCATGAATCAAAGG ACCCGGAAGGTTCTCTTGAGCTCTTTCTTAAGCCAATTTGGACGTTCAGTTTTTGACATTGGCTTCTGCA TGTCAATAACCATGTGGATTCATCTGAATCATGGAGACCATGGCCTATGGGAGTTCCTGGCCCATCTTTC CTCCCTCTGCCACTACCTCCTTGTGGAGCCCCAACCCTGGAAGTGTTACCGGGCAGCTGCAAGGCGTCTC CGAAAGCTGGGACTCCATGATTTTGACCACTTCCACTCCCTTGCCATCCGAGGTGACATGCCCAATCAGA TTGTGCAGATCTTGACCCAGGATCATGGCATGGAATTAATATGTTGCTTTGGCAACACCAGTTGGGACAG AAGCCTTCTGCTCTTCAGGGCAAAACAAACCATAGAGACTCATCCAATCCCTGAATCACTGATAGAAAAA GGGAAAGAAAAGAACAGATTAAGTTTCCAGAAGCAG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG208818 representing NM_181708
Red=Cloning site Green=Tags(s) MAVPTELDGGSVKETAAEEESRVLAPGAAPFGNFPHYSRFHPPEQRLRLLPPELLRQLFPESPENGPILG LDVGCNSGDLSVALYKHFLSLPDGETCSDASREFRLLCCDIDPVLVKRAEKECPFPDALTFITLDFMNQR TRKVLLSSFLSQFGRSVFDIGFCMSITMWIHLNHGDHGLWEFLAHLSSLCHYLLVEPQPWKCYRAAARRL RKLGLHDFDHFHSLAIRGDMPNQIVQILTQDHGMELICCFGNTSWDRSLLLFRAKQTIETHPIPESLIEK GKEKNRLSFQKQ TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_181708 |
| ORF Size | 876 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_181708.3 |
| RefSeq Size | 1495 bp |
| RefSeq ORF | 879 bp |
| Locus ID | 144233 |
| UniProt ID | Q7Z5W3 |
| Gene Summary | This gene encodes an RNA methyltransferase which belongs to the rossmann fold methyltransferase family, and serves as a 5'-methylphosphate capping enzyme that is specific for cytoplasmic histidyl tRNA. The encoded protein contains an S-adenosylmethionine binding domain and uses the methyl group donor, S-adenosylmethionine. This gene is overexpressed in breast cancer cells, and is related to the tumorigenic phenotype and poor prognosis of breast cancer. The encoded protein is thought to promote the cellular invasion of breast cancer cells, by downregulating the expression of tumor suppressor miRNAs through the dimethylation of the 5-monophosphate of the corresponding precursor miRNAs. [provided by RefSeq, Apr 2017] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC208818 | BCDIN3D (Myc-DDK-tagged)-Human BCDIN3 domain containing (BCDIN3D) |
CNY 2,400.00 |
|
| RC208818L1 | Lenti ORF clone of Human BCDIN3 domain containing (BCDIN3D), Myc-DDK-tagged |
CNY 4,800.00 |
|
| RC208818L2 | Lenti ORF clone of Human BCDIN3 domain containing (BCDIN3D), mGFP tagged |
CNY 5,890.00 |
|
| RC208818L3 | Lenti ORF clone of Human BCDIN3 domain containing (BCDIN3D), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC208818L4 | Lenti ORF clone of Human BCDIN3 domain containing (BCDIN3D), mGFP tagged |
CNY 5,890.00 |
|
| SC126373 | BCDIN3D (untagged)-Human BCDIN3 domain containing (BCDIN3D) |
CNY 6,270.00 |
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