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EP1 (PTGER1) (NM_000955) Human Tagged ORF Clone
CAT＃: RG208597
- TrueORF®
PTGER1 (tGFP-tagged) - Human prostaglandin E receptor 1 (subtype EP1), 42kDa (PTGER1)
ORF Plasmid: DDK
"NM_000955" in other vectors (4)
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CNY 4,370.00
Product images
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | EP1 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG208597 representing NM_000955
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGCCCTTGCGGGCCCCTCAACCTGAGCCTGGCGGGCGAGGCGACCACATGCGCGGCGCCCTGGGTCC CCAACACGTCGGCCGTGCCGCCGTCGGGCGCTTCGCCCGCGCTGCCCATCTTCTCCATGACGCTGGGCGC CGTGTCCAACCTGCTGGCGCTGGCGCTGCTGGCGCAGGCCGCGGGCCGCCTGCGACGCCGCCGCTCGGCC GCCACCTTCCTGCTGTTCGTGGCCAGCCTGCTGGCCACCGACCTGGCGGGCCACGTGATCCCGGGCGCGC TGGTGCTGCGTCTGTACACTGCGGGGCGCGCTCCGGCCGGCGGGGCCTGCCACTTCCTGGGCGGCTGCAT GGTCTTCTTCGGCCTGTGCCCGCTGCTGCTGGGCTGTGGCATGGCCGTGGAGCGCTGCGTGGGCGTCACG CGGCCGCTGCTCCACGCCGCGCGGGTCTCGGTCGCCCGCGCGCGCCTGGCGCTGGCCGCGGTGGCCGCGG TGGCCTTGGCCGTGGCGCTGCTGCCGCTGGCGCGCGTGGGCCGCTATGAGCTGCAGTACCCGGGCACGTG GTGCTTCATCGGCCTGGGTCCCCCGGGCGGCTGGCGCCAGGCACTGCTTGCTGGCCTCTTCGCCAGCCTC GGCCTGGTCGCGCTCCTCGCCGCGCTGGTGTGCAACACGCTCAGCGGCCTGGCCCTGCTACGCGCCCGCT GGCGACGCCGCTCCCGACGGCCTCCCCCGGCCTCAGGCCCCGACAGCCGGCGTCGCTGGGGGGCGCACGG ACCCCGCTCGGCCTCCGCCTCGTCCGCCTCGTCCATCGCTTCGGCCTCCACCTTCTTTGGCGGCTCTCGG AGCAGCGGCTCGGCACGCAGAGCTCGCGCCCACGACGTGGAGATGGTGGGCCAGCTTGTCGGTATCATGG TGGTGTCGTGCATCTGCTGGAGCCCAATGCTGGTGTTGGTGGCGCTGGCCGTCGGCGGCTGGAGCTCTAC CTCCCTGCAGCGGCCACTATTCCTGGCCGTGCGCCTTGCCTCCTGGAACCAGATCCTGGACCCTTGGGTG TACATCCTACTGCGCCAGGCCGTGCTGCGCCAACTGCTTCGCCTCTTGCCCCCGAGGGCCGGAGCCAAGG GCGGCCCCGCGGGGCTGGGCCTAACACCGAGCGCCTGGGAGGCCAGCTCGCTGCGCAGCTCCCGGCACAG CGGCCTCAGCCACTTC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG208597 representing NM_000955
Red=Cloning site Green=Tags(s) MSPCGPLNLSLAGEATTCAAPWVPNTSAVPPSGASPALPIFSMTLGAVSNLLALALLAQAAGRLRRRRSA ATFLLFVASLLATDLAGHVIPGALVLRLYTAGRAPAGGACHFLGGCMVFFGLCPLLLGCGMAVERCVGVT RPLLHAARVSVARARLALAAVAAVALAVALLPLARVGRYELQYPGTWCFIGLGPPGGWRQALLAGLFASL GLVALLAALVCNTLSGLALLRARWRRRSRRPPPASGPDSRRRWGAHGPRSASASSASSIASASTFFGGSR SSGSARRARAHDVEMVGQLVGIMVVSCICWSPMLVLVALAVGGWSSTSLQRPLFLAVRLASWNQILDPWV YILLRQAVLRQLLRLLPPRAGAKGGPAGLGLTPSAWEASSLRSSRHSGLSHF TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_000955 |
| ORF Size | 1206 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_000955.2, NP_000946.2 |
| RefSeq Size | 1458 bp |
| RefSeq ORF | 1209 bp |
| Locus ID | 5731 |
| UniProt ID | P34995 |
| Domains | 7tm_1 |
| Protein Families | Druggable Genome, GPCR, Transmembrane |
| Protein Pathways | Calcium signaling pathway, Neuroactive ligand-receptor interaction |
| Gene Summary | The protein encoded by this gene is a member of the G protein-coupled receptor family. This protein is one of four receptors identified for prostaglandin E2 (PGE2). Through a phosphatidylinositol-calcium second messenger system, G-Q proteins mediate this receptor's activity. Knockout studies in mice suggested a role of this receptor in mediating algesia and in regulation of blood pressure. Studies in mice also suggested that this gene may mediate adrenocorticotropic hormone response to bacterial endotoxin. [provided by RefSeq, Jul 2008] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC208597 | PTGER1 (Myc-DDK-tagged)-Human prostaglandin E receptor 1 (subtype EP1), 42kDa (PTGER1) |
CNY 3,656.00 |
|
| RC208597L3 | Lenti-ORF clone of PTGER1 (Myc-DDK-tagged)-Human prostaglandin E receptor 1 (subtype EP1), 42kDa (PTGER1) |
CNY 5,890.00 |
|
| RC208597L4 | Lenti-ORF clone of PTGER1 (mGFP-tagged)-Human prostaglandin E receptor 1 (subtype EP1), 42kDa (PTGER1) |
CNY 6,056.00 |
|
| SC119559 | PTGER1 (untagged)-Human prostaglandin E receptor 1 (subtype EP1), 42kDa (PTGER1) |
CNY 3,656.00 |
