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MAGEE1 (NM_020932) Human Tagged ORF Clone
CAT＃: RG208544
- TrueORF®
MAGEE1 (tGFP-tagged) - Human melanoma antigen family E, 1 (MAGEE1)
ORF Plasmid: DDK
"NM_020932" in other vectors (4)
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CNY 7,890.00
Product images
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | DAMAGE; HCA1 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG208544 representing NM_020932
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCTCTGGTAAGCCAGAATTCGCGCCGCCGCCGCCGCCGCGTTGCAAAGGCTACTGCGCACAACAGCA GCTGGGGCGAAATGCAGGCCCCTAATGCCCCCGGTCTCCCCGCTGATGTGCCAGGCTCAGACGTCCCCCA GGGTCCCAGCGATTCCCAGATCCTCCAGGGCCTCTGCGCCTCTGAGGGCCCAAGCACCTCCGTTCTGCCC ACCTCCGCTGAGGGCCCAAGCACCTTTGTGCCGCCCACCATCTCTGAGGCCTCAAGCGCCTCCGGGCAGC CCACCATCTCTGAGGGACCTGGCACCTCCGTGCTGCCCACCCCCAGTGAGGGCCTAAGCACCTCCGGGCC TCCCACCATCTCTAAGGGGCTGTGCACCTCTGTGACGCTTGCCGCCTCTGAGGGCCGGAACACCTCCAGG CCGCCCACTTCCTCTGAGGAACCTAGCACCTCCGTGCCGCCCACCGCCTCTGAGGTACCGAGCACCTCCC TGCCGCCCACCCCTGGTGAGGGAACGAGCACCTCCGTGCCGCCCACAGCCTATGAGGGACCAAGCACCTC CGTGGTGCCCACCCCTGATGAGGGACCAAGCACCTCCGTGCTGCCTACACCTGGTGAGGGACCAGGCACC TCCGTGCCGCTCGCCGCCACTGAGGGCCTGAGCACCTCCGTGCAGGCCACTCCTGATGAGGGACCGAGCA CCTCCGTGCCGCCCACCGCCACTGAGGGCCTAAGCACCCCCGTGCCACCCACCCGTGATGAGGGACCGAG CACCTCCGTGCCGGCCACTCCTGGTGAGGGACCGAGCACCTCCGTGCTGCCCGCCGCCTCTGACGGACAA AGCATCTCCTTGGTGCCCACCCGCGGTAAGGGATCAAGCACCTCCGTGCCCCCCACCGCCACCGAGGGCC TGAGCACCTCCGTGCAGCCCACTGCTGGTGAGGGATCGAGCACCTCCGTGCCGCCCACCCCTGGTGGGGG ACTGAGCACCTCCGTGCCGCCCACCGCCACTGAGGAGTTGAGCACCTCCGTGCCGCCCACTCCCGGTGAG GGACCAAGCACTTCCGTACTGCCAATCCCCGGTGAGGGACTGAGCACCTCTGTGCCGCCCACCGCCTCTG ATGGATCGGACACCTCCGTGCCGCCCACTCCTGGTGAGGGCGCAAGCACCTTAGTGCAGCCCACCGCCCC TGACGGACCGGGAAGCTCCGTGCTGCCTAACCCTGGTGAGGGCCCGAGCACATTGTTTAGCTCTAGTGCT TCTGTGGACCGGAACCCCTCCAAGTGTTCCCTTGTTTTGCCAAGCCCTAGGGTAACCAAGGCCTCCGTGG ACTCAGATTCTGAGGGTCCTAAGGGTGCAGAAGGCCCTATAGAATTCGAGGTCCTGAGAGACTGTGAGAG CCCCAACTCCATTAGTATTATGGGCCTCAATACTTCCCGGGTTGCAATTACCCTGAAGCCCCAAGACCCT ATGGAACAGAACGTAGCTGAGCTGTTGCAGTTCCTGCTGGTGAAGGATCAGAGCAAGTACCCTATCCGGG AGTCTGAAATGCGGGAATATATTGTTAAAGAATATCGCAACCAGTTTCCTGAGATACTCAGGCGAGCAGC AGCCCACCTGGAGTGCATTTTTAGGTTTGAATTGAGAGAACTTGACCCTGAGGCACACACCTACATTCTG TTAAACAAACTGGGACCTGTGCCCTTTGAAGGGTTAGAAGAGAGCCCAAATGGGCCAAAGATGGGCCTCC TGATGATGATTCTAGGCCAAATATTCCTGAATGGCAACCAAGCCAAGGAGGCTGAGATTTGGGAAATGCT CTGGAGGATGGGGGTGCAGCGGGAAAGGAGGCTTTCCATTTTTGGGAACCCAAAGAGACTTCTGTCTGTG GAGTTTGTATGGCAGCGTTACTTAGACTACAGGCCAGTAACTGACTGTAAACCAGTGGAGTATGAGTTTT TCTGGGGCCCAAGATCCCACCTAGAAACCACCAAGATGAAAATTCTGAAGTTCATGGCGAAAATATATAA CAAAGATCCTATGGATTGGCCAGAGAAATACAACGAAGCTCTGGAAGAAGATGCTGCCAGAGCCTTTGCT GAGGGTTGGCAGGCTCTCCCTCACTTTAGGAGGCCCTTTTTTGAGGAAGCTGCTGCAGAGGTACCATCCC CTGATTCAGAGGTTTCCAGCTATTCCTCAAAATATGCCCCACATTCATGGCCTGAGTCAAGATTGGAGAG CAAGGCAAGGAAGCTGGTGCAGTTATTTCTGCTTATGGATTCAACTAAGCTGCCTATACCAAAGAAAGGA ATTCTGTACTACATTGGCCGAGAGTGCAGCAAAGTGTTCCCTGACCTCCTGAATCGTGCTGCCCGCACCC TGAACCATGTCTATGGGACAGAACTAGTGGTACTTGATCCCAGGAATCACTCCTATACTCTGTACAACCG AAGGGAGATGGAAGAAACTGAGGAGATCGTAGACAGTCCAAACAGGCCTGGCAACAACTTTTTGATGCAG GTCCTAAGCTTCATCTTTATTATGGGCAACCATGCCAGGGAGTCTGCAGTCTGGGCCTTTCTGCGGGGCT TAGGGGTTCAAGCTGGGAGAAAGCATGTGATTACCTGCAGATACTTGAGTCAGCGCTATATAGACAGTTT ACGGGTTCCTGACAGTGATCCAGTGCAATATGAGTTTGTATGGGGTCCTAGAGCCCGTTTGGAAACCTCT AAGATGAAAGCCTTGCGATATGTGGCCAGAATCCACAGAAAGGAACCACAGGACTGGCCACAGCAGTACA GGGAGGCAATGGAAGATGAGGCCAATAGAGCTGATGTTGGGCACAGGCAAATCTTTGTTCACAACTTCAG G ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG208544 representing NM_020932
Red=Cloning site Green=Tags(s) MSLVSQNSRRRRRRVAKATAHNSSWGEMQAPNAPGLPADVPGSDVPQGPSDSQILQGLCASEGPSTSVLP TSAEGPSTFVPPTISEASSASGQPTISEGPGTSVLPTPSEGLSTSGPPTISKGLCTSVTLAASEGRNTSR PPTSSEEPSTSVPPTASEVPSTSLPPTPGEGTSTSVPPTAYEGPSTSVVPTPDEGPSTSVLPTPGEGPGT SVPLAATEGLSTSVQATPDEGPSTSVPPTATEGLSTPVPPTRDEGPSTSVPATPGEGPSTSVLPAASDGQ SISLVPTRGKGSSTSVPPTATEGLSTSVQPTAGEGSSTSVPPTPGGGLSTSVPPTATEELSTSVPPTPGE GPSTSVLPIPGEGLSTSVPPTASDGSDTSVPPTPGEGASTLVQPTAPDGPGSSVLPNPGEGPSTLFSSSA SVDRNPSKCSLVLPSPRVTKASVDSDSEGPKGAEGPIEFEVLRDCESPNSISIMGLNTSRVAITLKPQDP MEQNVAELLQFLLVKDQSKYPIRESEMREYIVKEYRNQFPEILRRAAAHLECIFRFELRELDPEAHTYIL LNKLGPVPFEGLEESPNGPKMGLLMMILGQIFLNGNQAKEAEIWEMLWRMGVQRERRLSIFGNPKRLLSV EFVWQRYLDYRPVTDCKPVEYEFFWGPRSHLETTKMKILKFMAKIYNKDPMDWPEKYNEALEEDAARAFA EGWQALPHFRRPFFEEAAAEVPSPDSEVSSYSSKYAPHSWPESRLESKARKLVQLFLLMDSTKLPIPKKG ILYYIGRECSKVFPDLLNRAARTLNHVYGTELVVLDPRNHSYTLYNRREMEETEEIVDSPNRPGNNFLMQ VLSFIFIMGNHARESAVWAFLRGLGVQAGRKHVITCRYLSQRYIDSLRVPDSDPVQYEFVWGPRARLETS KMKALRYVARIHRKEPQDWPQQYREAMEDEANRADVGHRQIFVHNFR TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_020932 |
| ORF Size | 2871 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_020932.3 |
| RefSeq Size | 3628 bp |
| RefSeq ORF | 2874 bp |
| Locus ID | 57692 |
| UniProt ID | Q9HCI5 |
| Gene Summary | This gene encodes an alpha-dystrobrevin-associated MAGE (melanoma-associated antigen) protein, which is a member of the MAGE family. The protein contains a nuclear localization signal in the N-terminus, 30 12-amino acid repeats beginning at nt 60 with the consensus sequence ASEGPSTSVLPT, and two MAGE domains in the C-terminus. It may play a signaling role in brain, muscle, and peripheral nerve. This gene is located on X chromosome in a region containing loci linked to cognitive disability. [provided by RefSeq, Mar 2010] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC208544 | MAGEE1 (Myc-DDK-tagged)-Human melanoma antigen family E, 1 (MAGEE1) |
CNY 7,008.00 |
|
| RC208544L3 | Lenti ORF clone of Human melanoma antigen family E, 1 (MAGEE1), Myc-DDK-tagged |
CNY 9,030.00 |
|
| RC208544L4 | Lenti ORF clone of Human melanoma antigen family E, 1 (MAGEE1), mGFP tagged |
CNY 9,030.00 |
|
| SC125774 | MAGEE1 (untagged)-Human melanoma antigen family E, 1 (MAGEE1) |
CNY 7,016.00 |
