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THBS4 (NM_003248) Human Tagged ORF Clone
CAT＃: RG208408
- TrueORF®
THBS4 (tGFP-tagged) - Human thrombospondin 4 (THBS4)
ORF Plasmid: DDK
"NM_003248" in other vectors (4)
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CNY 7,890.00
Product images
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | TSP-4; TSP4 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG208408 representing NM_003248
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCTGGCCCCGCGCGGAGCCGCCGTCCTCCTGCTGCACCTGGTCCTGCAGCGGTGGCTAGCGGCAGGCG CCCAGGCCACCCCCCAGGTCTTTGACCTTCTCCCATCTTCCAGTCAGAGGCTAAACCCAGGCGCTCTGCT GCCAGTCCTGACAGACCCCGCCCTGAATGATCTCTATGTGATTTCCACCTTCAAGCTGCAGACTAAAAGT TCAGCCACCATCTTCGGTCTTTACTCTTCAACTGACAACAGTAAATATTTTGAATTTACTGTGATGGGAC GCTTAAACAAAGCCATCCTCCGTTACCTGAAGAACGATGGGAAGGTGCATTTGGTGGTTTTCAACAACCT GCAGCTGGCAGACGGAAGGCGGCACAGGATCCTCCTGAGGCTGAGCAATTTGCAGCGAGGGGCCGGCTCC CTAGAGCTCTACCTGGACTGCATCCAGGTGGATTCCGTTCACAATCTCCCCAGGGCCTTTGCTGGCCCCT CCCAGAAACCTGAGACCATTGAATTGAGGACTTTCCAGAGGAAGCCACAGGACTTCTTGGAAGAGCTGAA GCTGGTGGTGAGAGGCTCACTGTTCCAGGTGGCCAGCCTGCAAGACTGCTTCCTGCAGCAGAGTGAGCCA CTGGCTGCCACAGGCACAGGGGACTTTAACCGGCAGTTCTTGGGTCAAATGACACAATTAAACCAACTCC TGGGAGAGGTGAAGGACCTTCTGAGACAGCAGGTTAAGGAAACATCATTTTTGCGAAACACCATAGCTGA ATGCCAGGCTTGCGGTCCTCTCAAGTTTCAGTCTCCGACCCCAAGCACGGTGGTGCCCCCGGCTCCCCCT GCACCGCCAACACGCCCACCTCGTCGGTGTGACTCCAACCCATGTTTCCGAGGTGTCCAATGTACCGACA GTAGAGATGGCTTCCAGTGTGGGCCCTGCCCCGAGGGCTACACAGGAAACGGGATCACCTGTATTGATGT TGATGAGTGCAAATACCATCCCTGCTACCCGGGCGTGCACTGCATAAATTTGTCTCCTGGCTTCAGATGT GACGCCTGCCCAGTGGGCTTCACAGGGCCCATGGTGCAGGGTGTTGGGATCAGTTTTGCCAAGTCAAACA AGCAGGTCTGCACTGACATTGATGAGTGTCGAAATGGAGCGTGCGTTCCCAACTCGATCTGCGTTAATAC TTTGGGATCTTACCGCTGTGGGCCTTGTAAGCCGGGGTATACTGGTGATCAGATAAGGGGATGCAAAGCG GAAAGAAACTGCAGAAACCCAGAGCTGAACCCTTGCAGTGTGAATGCCCAGTGCATTGAAGAGAGGCAGG GGGATGTGACATGTGTGTGTGGAGTCGGTTGGGCTGGAGATGGCTATATCTGTGGAAAGGATGTGGACAT CGACAGTTACCCCGACGAAGAACTGCCATGCTCTGCCAGGAACTGTAAAAAGGACAACTGCAAATATGTG CCAAATTCTGGCCAAGAAGATGCAGACAGAGATGGCATTGGCGACGCTTGTGACGAGGATGCTGACGGAG ATGGGATCCTGAATGAGCAGGATAACTGTGTCCTGATTCATAATGTGGACCAAAGGAACAGCGATAAAGA TATCTTTGGGGATGCCTGTGATAACTGCCTGAGTGTCTTAAATAACGACCAGAAAGACACCGATGGGGAT GGAAGAGGAGATGCCTGTGATGATGACATGGATGGAGATGGAATAAAAAACATTCTGGACAACTGCCCAA AATTTCCCAATCGTGACCAACGGGACAAGGATGGTGATGGTGTGGGGGATGCCTGTGACAGTTGTCCTGA TGTCAGCAACCCTAACCAGTCTGATGTGGATAATGATCTGGTTGGGGACTCCTGTGACACCAATCAGGAC AGTGATGGAGATGGGCACCAGGACAGCACAGACAACTGCCCCACCGTCATTAACAGTGCCCAGCTGGACA CCGATAAGGATGGAATTGGTGACGAGTGTGATGATGATGATGACAATGATGGTATCCCAGACCTGGTGCC CCCTGGACCAGACAACTGCCGGCTGGTCCCCAACCCAGCCCAGGAGGATAGCAACAGCGACGGAGTGGGA GACATCTGTGAGTCTGACTTTGACCAGGACCAGGTCATCGATCGGATCGACGTCTGCCCAGAGAACGCAG AGGTCACCCTGACCGACTTCAGGGCTTACCAGACCGTGGTCCTGGATCCTGAAGGGGATGCCCAGATCGA TCCCAACTGGGTGGTCCTGAACCAGGGCATGGAGATTGTACAGACCATGAACAGTGATCCTGGCCTGGCA GTGGGGTACACAGCTTTTAATGGAGTTGACTTCGAAGGGACCTTCCATGTGAATACCCAGACAGATGATG ACTATGCAGGCTTTATCTTTGGCTACCAAGATAGCTCCAGCTTCTACGTGGTCATGTGGAAGCAGACGGA GCAGACATATTGGCAAGCCACCCCATTCCGAGCAGTTGCAGAACCTGGCATTCAGCTCAAGGCTGTGAAG TCTAAGACAGGTCCAGGGGAGCATCTCCGGAACTCCCTGTGGCACACGGGGGACACCAGTGACCAGGTCA GGCTGCTGTGGAAGGACTCCAGGAATGTGGGCTGGAAGGACAAGGTGTCCTACCGCTGGTTCCTACAGCA CAGGCCCCAGGTGGGCTACATCAGGGTACGATTTTATGAAGGCTCTGAGTTGGTGGCTGACTCTGGCGTC ACCATAGACACCACAATGCGTGGAGGCCGACTTGGCGTTTTCTGCTTCTCTCAAGAAAACATCATCTGGT CCAACCTCAAGTATCGCTGCAATGACACCATCCCTGAGGACTTCCAAGAGTTTCAAACCCAGAATTTCGA CCGCTTCGATAAT ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG208408 representing NM_003248
Red=Cloning site Green=Tags(s) MLAPRGAAVLLLHLVLQRWLAAGAQATPQVFDLLPSSSQRLNPGALLPVLTDPALNDLYVISTFKLQTKS SATIFGLYSSTDNSKYFEFTVMGRLNKAILRYLKNDGKVHLVVFNNLQLADGRRHRILLRLSNLQRGAGS LELYLDCIQVDSVHNLPRAFAGPSQKPETIELRTFQRKPQDFLEELKLVVRGSLFQVASLQDCFLQQSEP LAATGTGDFNRQFLGQMTQLNQLLGEVKDLLRQQVKETSFLRNTIAECQACGPLKFQSPTPSTVVPPAPP APPTRPPRRCDSNPCFRGVQCTDSRDGFQCGPCPEGYTGNGITCIDVDECKYHPCYPGVHCINLSPGFRC DACPVGFTGPMVQGVGISFAKSNKQVCTDIDECRNGACVPNSICVNTLGSYRCGPCKPGYTGDQIRGCKA ERNCRNPELNPCSVNAQCIEERQGDVTCVCGVGWAGDGYICGKDVDIDSYPDEELPCSARNCKKDNCKYV PNSGQEDADRDGIGDACDEDADGDGILNEQDNCVLIHNVDQRNSDKDIFGDACDNCLSVLNNDQKDTDGD GRGDACDDDMDGDGIKNILDNCPKFPNRDQRDKDGDGVGDACDSCPDVSNPNQSDVDNDLVGDSCDTNQD SDGDGHQDSTDNCPTVINSAQLDTDKDGIGDECDDDDDNDGIPDLVPPGPDNCRLVPNPAQEDSNSDGVG DICESDFDQDQVIDRIDVCPENAEVTLTDFRAYQTVVLDPEGDAQIDPNWVVLNQGMEIVQTMNSDPGLA VGYTAFNGVDFEGTFHVNTQTDDDYAGFIFGYQDSSSFYVVMWKQTEQTYWQATPFRAVAEPGIQLKAVK SKTGPGEHLRNSLWHTGDTSDQVRLLWKDSRNVGWKDKVSYRWFLQHRPQVGYIRVRFYEGSELVADSGV TIDTTMRGGRLGVFCFSQENIIWSNLKYRCNDTIPEDFQEFQTQNFDRFDN TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_003248 |
| ORF Size | 2883 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_003248.6 |
| RefSeq Size | 3233 bp |
| RefSeq ORF | 2886 bp |
| Locus ID | 7060 |
| UniProt ID | P35443 |
| Domains | EGF_CA, TSPN, tsp_3, EGF, EGF |
| Protein Families | Druggable Genome |
| Protein Pathways | ECM-receptor interaction, Focal adhesion, TGF-beta signaling pathway |
| Gene Summary | The protein encoded by this gene belongs to the thrombospondin protein family. Thrombospondin family members are adhesive glycoproteins that mediate cell-to-cell and cell-to-matrix interactions. This protein forms a pentamer and can bind to heparin and calcium. It is involved in local signaling in the developing and adult nervous system, and it contributes to spinal sensitization and neuropathic pain states. This gene is activated during the stromal response to invasive breast cancer. It may also play a role in inflammatory responses in Alzheimer's disease. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Apr 2015] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC208408 | THBS4 (Myc-DDK-tagged)-Human thrombospondin 4 (THBS4) |
CNY 7,040.00 |
|
| RC208408L3 | Lenti-ORF clone of THBS4 (Myc-DDK-tagged)-Human thrombospondin 4 (THBS4) |
CNY 9,440.00 |
|
| RC208408L4 | Lenti-ORF clone of THBS4 (mGFP-tagged)-Human thrombospondin 4 (THBS4) |
CNY 9,030.00 |
|
| SC118077 | THBS4 (untagged)-Human thrombospondin 4 (THBS4) |
CNY 10,568.00 |
