TTC21B (NM_024753) Human Tagged ORF Clone
CAT#: RG207978
- TrueORF®
TTC21B (tGFP-tagged) - Human tetratricopeptide repeat domain 21B (TTC21B)
ORF Plasmid: DDK
"NM_024753" in other vectors (7)
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CNY 15,344.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | TurboGFP |
Synonyms | ATD4; FAP60; FLA17; IFT139; IFT139B; JBTS11; Nbla10696; NPHP12; SRTD4; THM1 |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RG207978 representing NM_024753
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGACTCGCAGGAATTGAAGACTTTGATTAATTACTATTGTCAAGAGAGATATTTCCATCATGTATTAC TGGTTGCCAGTGAAGGAATTAAGAGGTATGGAAGTGATCCAGTCTTCAGGTTTTATCATGCCTATGGCAC ATTAATGGAAGGTAAAACTCAAGAAGCTCTTCGAGAATTTGAGGCTATTAAAAATAAACAAGATGTATCA CTTTGTTCTCTACTTGCACTGATATATGCCCATAAAATGAGTCCTAATCCAGATAGAGAAGCTATTCTGG AATCAGATGCCAGAGTGAAGGAACAACGTAAAGGAGCTGGAGAGAAAGCCTTATACCATGCAGGCTTATT TTTATGGCACATTGGTCGCCATGATAAAGCAAGGGAATATATTGACAGAATGATCAAAATATCAGATGGT AGTAAACAGGGACACGTTTTGAAAGCATGGCTTGATATTACAAGAGGAAAAGAGCCTTACACTAAAAAAG CACTGAAGTATTTTGAAGAGGGACTCCAAGATGGGAATGATACTTTTGCTCTGCTGGGTAAGGCACAATG CCTTGAGATGCGCCAGAATTATTCAGGTGCCCTGGAGACTATGAACCAGATAATCGTGAATTTTCCGAGC TTCCTTCCTGCTTTTGTTAAGAAAATGAAATTACAACTAGCCTTGCAGGATTGGGACCAGACAGTTGAGA CAGCACAAAGGTTGCTGCTCCAAGATAGCCAAAATGTGGAAGCACTGAGAATGCAGGCACTCTACTATGT GTGTAGAGAGGGGGATATAGAGAAGGCTTCCACCAAGCTGGAAAACTTGGGAAATGCATTGGATGCCATG GAACCACAGAATGCTCAACTTTTCTATAACATTACACTCGCCTTCAGCAGAACTTGTGGACGTAGTCAAC TTATTCTTCAAAAAATTCAAACGTTACTTGAGAGAGCTTTTAGTTTAAACCCTCAGCAATCAGAATTTGC TACAGAACTTGGATACCAAATGATTTTACAAGGAAGAGTTAAAGAGGCACTGAAGTGGTATAAGACCGCC ATGACACTTGATGAGACTAGTGTGTCTGCCCTAGTTGGATTTATCCAATGTCAGTTGATAGAAGGGCAAT TACAGGATGCAGATCAGCAGCTAGAATTTTTAAATGAAATCCAGCAATCCATTGGAAAATCTGCGGAATT AATCTATTTACATGCAGTTCTTGCCATGAAGAAAAATAAACGACAAGAAGAAGTTATTAATTTGTTAAAT GATGTCCTGGACACTCACTTTTCACAATTAGAAGGTTTGCCTCTTGGCATACAGTATTTTGAAAAGCTAA ATCCTGATTTCTTGTTAGAAATTGTTATGGAGTATCTGAGCTTCTGTCCAATGCAGCCTGCAAGTCCTGG GCAACCTCTTTGTCCACTTCTCAGGCGTTGCATCTCAGTCCTGGAGACTGTAGTAAGAACTGTTCCAGGT CTTCTGCAAACAGTCTTCCTAATAGCAAAAGTGAAATATTTGTCAGGTGATATTGAAGCAGCTTTCAATA ACCTTCAGCACTGCTTAGAACACAATCCCTCTTATGCTGATGCTCATCTGCTGCTAGCTCAGGTTTACTT GTCTCAAGAAAAAGTCAAATTGTGTTCTCAGTCTCTTGAACTTTGTCTGAGCTATGATTTTAAGGTGAGA GACTATCCTTTATACCATTTGATAAAAGCTCAGTCACAAAAGAAAATGGGAGAAATAGCAGACGCAATTA AAACACTGCATATGGCAATGAGTTTACCAGGAATGAAAAGAATTGGAGCTTCCACAAAATCAAAAGACAG AAAAACTGAAGTTGATACAAGCCATCGTTTATCGATCTTTCTTGAATTGATAGACGTTCACCGCTTAAAT GGAGAGCAGCATGAGGCAACCAAAGTTTTACAAGATGCCATCCATGAATTTTCTGGAACATCTGAAGAAG TGCGGGTTACCATTGCTAATGCAGACCTTGCTCTAGCCCAAGGAGATATTGAACGGGCTTTAAGCATCCT TCAGAATGTTACAGCCGAACAGCCTTATTTTATAGAGGCCAGAGAAAAAATGGCAGATATTTATCTGAAG CACAGAAAAGATAAAATGTTATATATCACTTGTTTTAGAGAAATTGCTGAAAGAATGGCTAACCCTCGGT CTTTTCTTCTCCTTGGTGATGCATACATGAATATTCTAGAGCCTGAAGAAGCCATAGTAGCATATGAGCA AGCATTAAATCAGAACCCGAAAGATGGAACATTGGCAAGCAAAATGGGCAAAGCACTTATCAAAACTCAT AACTACTCAATGGCAATCACTTACTATGAAGCTGCTCTGAAAACTGGACAAAAGAATTATCTTTGCTATG ACCTGGCTGAGCTCTTATTAAAATTGAAATGGTATGACAAAGCAGAAAAAGTTCTTCAGCATGCTCTGGC TCATGAACCTGTAAATGAACTGTCAGCTCTCATGGAGGATGGACGTTGTCAAGTTCTTCTAGCAAAAGTT TATAGTAAAATGGAAAAACTTGGTGATGCGATCACTGCATTACAACAGGCTCGAGAATTACAAGCTCGGG TACTAAAACGTGTTCAGATGGAACAGCCAGATGCAGTTCCTGCACAGAAACATTTAGCAGCTGAAATTTG TGCAGAGATTGCAAAACATTCTGTTGCTCAGCGAGACTATGAAAAAGCAATTAAGTTTTATAGAGAGGCT CTGGTTCACTGCGAAACAGATAATAAGATTATGTTGGAACTGGCACGATTATACCTGGCACAAGATGACC CTGATTCCTGCCTGCGGCAGTGTGCTCTACTGCTTCAGAGTGACCAGGATAACGAAGCTGCTACCATGAT GATGGCTGATCTCATGTTCAGAAAACAAGACTATGAACAAGCAGTGTTTCATTTACAGCAGCTTTTAGAA CGTAAGCCAGATAATTATATGACATTATCTCGTTTGATTGATCTCCTAAGAAGATGTGGAAAACTCGAGG ATGTCCCAAGATTTTTCTCAATGGCTGAGAAACGTAACTCCAGAGCAAAATTGGAACCAGGATTTCAGTA TTGTAAAGGACTGTATCTTTGGTACACTGGAGAACCAAATGATGCCCTTCGACATTTTAATAAAGCTCGG AAAGATCGTGACTGGGGCCAAAATGCCCTTTATAATATGATAGAGATCTGTTTGAATCCAGATAATGAAA CTGTTGGAGGTGAAGTATTTGAAAACCTGGATGGAGACCTGGGTAATTCAACTGAGAAGCAAGAATCTGT GCAACTGGCAGTAAGAACAGCAGAAAAACTTCTTAAGGAACTAAAACCTCAGACTGTTCAGGGTCACGTA CAGCTTCGCATAATGGAAAACTATTGCTTAATGGCTACCAAACAGAAATCTAATGTTGAACAAGCATTAA ATACCTTCACTGAAATAGCAGCATCTGAGAAGGAGCATATCCCAGCGCTCTTGGGAATGGCAACGGCTTA TATGATCTTGAAACAGACTCCACGAGCCAGAAACCAGCTGAAGCGTATTGCGAAAATGAATTGGAATGCT ATTGATGCTGAAGAGTTTGAGAAGAGTTGGCTGCTACTTGCTGATATTTACATTCAATCAGCAAAATATG ACATGGCAGAAGACCTGTTAAAACGGTGCCTGCGTCATAATAGATCTTGCTGCAAAGCTTATGAATATAT GGGATACATTATGGAAAAAGAGCAAGCATATACAGATGCTGCCTTGAACTATGAGATGGCATGGAAATAT AGCAATCGGACAAATCCGGCAGTAGGATACAAACTGGCATTTAATTACTTAAAAGCAAAAAGATATGTGG ATTCAATTGACATATGTCACCAGGTTCTTGAAGCACATCCAACTTATCCAAAAATCAGAAAGGATATACT TGATAAGGCCCGTGCGTCTTTAAGACCT ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG207978 representing NM_024753
Red=Cloning site Green=Tags(s) MDSQELKTLINYYCQERYFHHVLLVASEGIKRYGSDPVFRFYHAYGTLMEGKTQEALREFEAIKNKQDVS LCSLLALIYAHKMSPNPDREAILESDARVKEQRKGAGEKALYHAGLFLWHIGRHDKAREYIDRMIKISDG SKQGHVLKAWLDITRGKEPYTKKALKYFEEGLQDGNDTFALLGKAQCLEMRQNYSGALETMNQIIVNFPS FLPAFVKKMKLQLALQDWDQTVETAQRLLLQDSQNVEALRMQALYYVCREGDIEKASTKLENLGNALDAM EPQNAQLFYNITLAFSRTCGRSQLILQKIQTLLERAFSLNPQQSEFATELGYQMILQGRVKEALKWYKTA MTLDETSVSALVGFIQCQLIEGQLQDADQQLEFLNEIQQSIGKSAELIYLHAVLAMKKNKRQEEVINLLN DVLDTHFSQLEGLPLGIQYFEKLNPDFLLEIVMEYLSFCPMQPASPGQPLCPLLRRCISVLETVVRTVPG LLQTVFLIAKVKYLSGDIEAAFNNLQHCLEHNPSYADAHLLLAQVYLSQEKVKLCSQSLELCLSYDFKVR DYPLYHLIKAQSQKKMGEIADAIKTLHMAMSLPGMKRIGASTKSKDRKTEVDTSHRLSIFLELIDVHRLN GEQHEATKVLQDAIHEFSGTSEEVRVTIANADLALAQGDIERALSILQNVTAEQPYFIEAREKMADIYLK HRKDKMLYITCFREIAERMANPRSFLLLGDAYMNILEPEEAIVAYEQALNQNPKDGTLASKMGKALIKTH NYSMAITYYEAALKTGQKNYLCYDLAELLLKLKWYDKAEKVLQHALAHEPVNELSALMEDGRCQVLLAKV YSKMEKLGDAITALQQARELQARVLKRVQMEQPDAVPAQKHLAAEICAEIAKHSVAQRDYEKAIKFYREA LVHCETDNKIMLELARLYLAQDDPDSCLRQCALLLQSDQDNEAATMMMADLMFRKQDYEQAVFHLQQLLE RKPDNYMTLSRLIDLLRRCGKLEDVPRFFSMAEKRNSRAKLEPGFQYCKGLYLWYTGEPNDALRHFNKAR KDRDWGQNALYNMIEICLNPDNETVGGEVFENLDGDLGNSTEKQESVQLAVRTAEKLLKELKPQTVQGHV QLRIMENYCLMATKQKSNVEQALNTFTEIAASEKEHIPALLGMATAYMILKQTPRARNQLKRIAKMNWNA IDAEEFEKSWLLLADIYIQSAKYDMAEDLLKRCLRHNRSCCKAYEYMGYIMEKEQAYTDAALNYEMAWKY SNRTNPAVGYKLAFNYLKAKRYVDSIDICHQVLEAHPTYPKIRKDILDKARASLRP TRTRPLE - GFP Tag - V |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_024753 |
ORF Size | 3948 bp |
OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_024753.2 |
RefSeq Size | 4916 bp |
RefSeq ORF | 3951 bp |
Locus ID | 79809 |
UniProt ID | Q7Z4L5 |
Domains | TPR |
Protein Families | Protease |
Gene Summary | This gene encodes a member of TTC21 family, containing several tetratricopeptide repeat (TPR) domains. This protein is localized to the cilium axoneme, and may play a role in retrograde intraflagellar transport in cilia. Mutations in this gene are associated with various ciliopathies, nephronophthisis 12, and asphyxiating thoracic dystrophy 4. [provided by RefSeq, Oct 2011] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC207978 | TTC21B (Myc-DDK-tagged)-Human tetratricopeptide repeat domain 21B (TTC21B) |
CNY 13,744.00 |
|
RC207978L1 | Lenti ORF clone of Human tetratricopeptide repeat domain 21B (TTC21B), Myc-DDK-tagged |
CNY 16,144.00 |
|
RC207978L2 | Lenti ORF clone of Human tetratricopeptide repeat domain 21B (TTC21B), mGFP tagged |
CNY 11,500.00 |
|
RC207978L3 | Lenti ORF clone of Human tetratricopeptide repeat domain 21B (TTC21B), Myc-DDK-tagged |
CNY 11,500.00 |
|
RC207978L4 | Lenti ORF clone of Human tetratricopeptide repeat domain 21B (TTC21B), mGFP tagged |
CNY 11,500.00 |
|
SC108789 | TTC21B (untagged)-Human tetratricopeptide repeat domain 21B (TTC21B) |
CNY 3,600.00 |
|
SC318594 | TTC21B (untagged)-Human tetratricopeptide repeat domain 21B (TTC21B) |
CNY 13,752.00 |