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BOULE (BOLL) (NM_033030) Human Tagged ORF Clone
CAT＃: RG207436
- TrueORF®
BOLL (tGFP-tagged) - Human bol, boule-like (Drosophila) (BOLL), transcript variant 2
ORF Plasmid: DDK
"NM_033030" in other vectors (6)
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CNY 4,000.00
CNY 4,370.00
Product images
CNY 1,000.00
CNY 1,999.00
CNY 2,700.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | BOULE |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG207436 representing NM_033030
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCAAACAGATTCATTATCTCCATCCCCTAATCCTGTGTCACCTGTGCCTTTGAATAACCCAACAAGTG CCCCAAGATATGGAACAGTGATCCCTAATCGCATCTTTGTAGGAGGAATTGATTTTAAGACAAACGAAAG TGATTTAAGAAAATTTTTTTCCCAGTATGGGTCTGTGAAAGAAGTGAAGATTGTAAATGACAGAGCTGGA GTATCCAAAGGGTATGGTTTCGTCACTTTTGAAACACAAGAAGATGCACAAAAAATTTTACAAGAGGCTG AAAAACTTAATTATAAGGATAAGAAGCTGAACATTGGTCCAGCAATAAGAAAACAACAAGTAGGGATCCC TCGTTCTAGTATAATGCCAGCAGCTGGAACAATGTATCTAACAACTTCAACTGGATATCCTTATACTTAC CATAATGGTGTTGCTTATTTTCATACTCCAGAGGTAACTTCGGTCCCACCGCCTTGGCCTTCACGTTCTG TATGTAGCTCCCCTGTGATGGTAGCTCAGCCCATTTATCAGCAACCTGCATATCACTACCAGGCCACCAC ACAGTATTTACCAGGACAGTGGCAGTGGAGTGTTCCTCAGCCTTCTGCCTCTTCTGCTCCATTCTTATAC CTGCAACCTTCTGAGGTTATTTATCAACCAGTGGAAATTGCACAGGATGGTGGATGTGTTCCTCCTCCAC TGTCTCTGATGGAAACTTCAGTTCCAGAGCCTTATTCTGATCATGGAGTTCAAGCAACATATCACCAGGT TTATGCTCCAAGTGCCATCACTATGCCTGCGCCTGTGATGCAGCCTGAGCCAATTAAAACAGTGTGGAGC ATTCATTAT ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG207436 representing NM_033030
Red=Cloning site Green=Tags(s) MQTDSLSPSPNPVSPVPLNNPTSAPRYGTVIPNRIFVGGIDFKTNESDLRKFFSQYGSVKEVKIVNDRAG VSKGYGFVTFETQEDAQKILQEAEKLNYKDKKLNIGPAIRKQQVGIPRSSIMPAAGTMYLTTSTGYPYTY HNGVAYFHTPEVTSVPPPWPSRSVCSSPVMVAQPIYQQPAYHYQATTQYLPGQWQWSVPQPSASSAPFLY LQPSEVIYQPVEIAQDGGCVPPPLSLMETSVPEPYSDHGVQATYHQVYAPSAITMPAPVMQPEPIKTVWS IHY TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_033030 |
| ORF Size | 849 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_033030.6 |
| RefSeq Size | 2867 bp |
| RefSeq ORF | 852 bp |
| Locus ID | 66037 |
| UniProt ID | Q8N9W6 |
| Gene Summary | This gene belongs to the DAZ gene family required for germ cell development. It encodes an RNA-binding protein which is more similar to Drosophila Boule than to human proteins encoded by genes DAZ (deleted in azoospermia) or DAZL (deleted in azoospermia-like). Loss of this gene function results in the absence of sperm in semen (azoospermia). Histological studies demonstrated that the primary defect is at the meiotic G2/M transition. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, Jul 2008] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC207436 | BOLL (Myc-DDK-tagged)-Human bol, boule-like (Drosophila) (BOLL), transcript variant 2 |
CNY 2,400.00 |
|
| RC207436L1 | Lenti ORF clone of Human bol, boule-like (Drosophila) (BOLL), transcript variant 2, Myc-DDK-tagged |
CNY 4,800.00 |
|
| RC207436L2 | Lenti ORF clone of Human bol, boule-like (Drosophila) (BOLL), transcript variant 2, mGFP tagged |
CNY 5,890.00 |
|
| RC207436L3 | Lenti ORF clone of Human bol, boule-like (Drosophila) (BOLL), transcript variant 2, Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC207436L4 | Lenti ORF clone of Human bol, boule-like (Drosophila) (BOLL), transcript variant 2, mGFP tagged |
CNY 5,890.00 |
|
| SC123074 | BOLL (untagged)-Human bol, boule-like (Drosophila) (BOLL), transcript variant 2 |
CNY 2,400.00 |
