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AFAP (AFAP1) (NM_021638) Human Tagged ORF Clone
CAT＃: RG207421
- TrueORF®
AFAP1 (tGFP-tagged) - Human actin filament associated protein 1 (AFAP1), transcript variant 1
ORF Plasmid: DDK
"NM_021638" in other vectors (6)
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CNY 6,944.00
CNY 8,080.00
Product images
CNY 1,999.00
CNY 2,700.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | actin filament-associated protein, 110 kDa; actin filament associated protein 1; AFAP; AFAP, AFAP-110; AFAP-110; FLJ56849; OTTHUMP00000155170 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG207421 representing NM_021638
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAAGAGTTAATAGTTGAACTTCGTCTCTTTCTTGAACTCCTGGACCATGAATATCTAACCTCAACTG TCAGGGAGAAAAAGGCAGTGATAACCAACATTCTGCTAAGAATACAGTCATCCAAAGGTTTTGATGTGAA GGACCATGCTCAGAAGCAGGAGACCGCTAACAGCCTGCCAGCCCCTCCTCAGATGCCCCTGCCGGAGATC CCTCAGCCCTGGCTGCCTCCTGACAGTGGGCCTCCACCATTGCCAACATCCTCCCTCCCAGAAGGTTATT ATGAGGAAGCTGTGCCACTGAGCCCCGGAAAAGCTCCGGAATACATCACATCAAATTATGATTCCGATGC GATGAGCAGCTCTTATGAGTCGTATGATGAAGAGGAGGAGGATGGGAAGGGGAAGAAAACCCGGCACCAG TGGCCCTCCGAGGAGGCCTCCATGGACCTGGTCAAGGACGCCAAAATCTGCGCCTTCCTGCTGCGGAAGA AGCGGTTCGGCCAGTGGACCAAGTTGCTCTGCGTCATCAAAGACACCAAACTGCTGTGCTATAAAAGTTC CAAGGACCAGCAGCCTCAGATGGAACTGCCACTCCAAGGCTGTAACATTACGTACATCCCGAAAGACAGC AAAAAGAAGAAGCACGAGCTGAAGATTACTCAGCAGGGCACGGACCCGCTTGTTCTCGCCGTCCAGAGCA AGGAACAGGCCGAGCAGTGGCTGAAGGTGATCAAAGAAGCCTACAGTGGTTGTAGTGGCCCCGTGGATTC AGAGTGTCCTCCTCCACCAAGCTCCCCGGTGCACAAGGCAGAACTGGAGAAGAAACTGTCTTCAGAGAGA CCCAGCTCAGATGGGGAGGGTGTTGTGGAAAATGGAATTACCACATGTAATGGAAAGGAGCAAGTGAAGA GGAAGAAAAGTTCCAAATCAGAGGCCAAGGGCACTGTGTCGAAAGTCACTGGGAAAAAAATCACCAAGAT CATCAGTCTGGGAAAGAAAAAGCCGTCCACAGACGAGCAGACCTCCTCAGCTGAGGAAGATGTTCCCACC TGCGGCTATCTGAACGTGCTCTCCAACAGCCGCTGGCGAGAGCGCTGGTGCCGAGTGAAAGATAACAAGC TCATTTTCCACAAGGACAGGACCGACCTGAAGACCCATATTGTGTCTATTCCGCTCCGTGGCTGCGAGGT GATCCCGGGTTTGGATTGTAAACATCCTCTGACGTTCCGGCTGCTGCGCAACGGCCAGGAGGTTGCAGTA TTGGAGGCATCTTCTTCTGAAGACATGGGCAGGTGGATTGGGATTTTACTCGCAGAGACGGGATCGTCCA CAGACCCGGAGGCTCTGCACTATGACTACATTGATGTGGAGATGTCTGCAAGTGTCATTCAGACAGCCAA ACAGACCTTCTGTTTCATGAACAGGCGTGTTATATCTGCTAACCCATATCTAGGGGGCACCTCCAACGGC TATGCCCACCCCAGCGGGACGGCACTTCATTATGACGATGTCCCGTGCATCAACGGCTCGCTCAAGGGTA AAAAGCCCCCCGTGGCGTCTAATGGGGTCACAGGAAAAGGGAAGACTCTGAGCAGTCAGCCAAAGAAAGC GGATCCCGCGGCTGTTGTGAAAAGGACGGGTTCGAATGCTGCCCAGTACAAGTATGGCAAGAACCGGGTA GAAGCAGATGCCAAGCGGCTACAGACCAAAGAGGAGGAGCTGCTGAAGAGGAAAGAGGCCCTGCGGAATA GGCTGGCCCAGCTCCGCAAGGAAAGAAAAGACCTTCGAGCGGCTATTGAAGTGAACGCCGGCAGGAAGCC GCAGGCGATCCTGGAGGAGAAGCTGAAGCAGCTGGAGGAGGAGTGCCGGCAGAAGGAGGCGGAGCGTGTC AGCCTGGAGCTGGAGCTGACGGAGGTCAAGGAGAGCCTGAAGAAAGCGCTGGCGGGCGGAGTCACCCTGG GGCTGGCCATCGAGCCCAAGTCAGGGACATCGAGTCCACAGTCTCCAGTGTTCCGGCACCGGACCCTGGA AAACTCGCCCATCTCCAGCTGTGACACCAGTGACACCGAGGGCCCCGTGCCGGTGAACAGCGCGGCCGTC TTGAAGAAGAGCCAGGCTGCCCCGGGCAGCTCCCCCTGCCGAGGGCATGTGCTGCGGAAGGCCAAGGAAT GGGAATTGAAGAACGGGACC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG207421 representing NM_021638
Red=Cloning site Green=Tags(s) MEELIVELRLFLELLDHEYLTSTVREKKAVITNILLRIQSSKGFDVKDHAQKQETANSLPAPPQMPLPEI PQPWLPPDSGPPPLPTSSLPEGYYEEAVPLSPGKAPEYITSNYDSDAMSSSYESYDEEEEDGKGKKTRHQ WPSEEASMDLVKDAKICAFLLRKKRFGQWTKLLCVIKDTKLLCYKSSKDQQPQMELPLQGCNITYIPKDS KKKKHELKITQQGTDPLVLAVQSKEQAEQWLKVIKEAYSGCSGPVDSECPPPPSSPVHKAELEKKLSSER PSSDGEGVVENGITTCNGKEQVKRKKSSKSEAKGTVSKVTGKKITKIISLGKKKPSTDEQTSSAEEDVPT CGYLNVLSNSRWRERWCRVKDNKLIFHKDRTDLKTHIVSIPLRGCEVIPGLDCKHPLTFRLLRNGQEVAV LEASSSEDMGRWIGILLAETGSSTDPEALHYDYIDVEMSASVIQTAKQTFCFMNRRVISANPYLGGTSNG YAHPSGTALHYDDVPCINGSLKGKKPPVASNGVTGKGKTLSSQPKKADPAAVVKRTGSNAAQYKYGKNRV EADAKRLQTKEEELLKRKEALRNRLAQLRKERKDLRAAIEVNAGRKPQAILEEKLKQLEEECRQKEAERV SLELELTEVKESLKKALAGGVTLGLAIEPKSGTSSPQSPVFRHRTLENSPISSCDTSDTEGPVPVNSAAV LKKSQAAPGSSPCRGHVLRKAKEWELKNGT TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_021638 |
| ORF Size | 2190 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_021638.4, NP_067651.2 |
| RefSeq Size | 7479 bp |
| RefSeq ORF | 2192 bp |
| Locus ID | 60312 |
| Domains | PH |
| Gene Summary | The protein encoded by this gene is a Src binding partner. It may represent a potential modulator of actin filament integrity in response to cellular signals, and may function as an adaptor protein by linking Src family members and/or other signaling proteins to actin filaments. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2008] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC207421 | AFAP1 (Myc-DDK-tagged)-Human actin filament associated protein 1 (AFAP1), transcript variant 1 |
CNY 5,344.00 |
|
| RC207421L1 | Lenti ORF clone of Human actin filament associated protein 1 (AFAP1), transcript variant 1, Myc-DDK-tagged |
CNY 7,744.00 |
|
| RC207421L2 | Lenti ORF clone of Human actin filament associated protein 1 (AFAP1), transcript variant 1, mGFP tagged |
CNY 9,220.00 |
|
| RC207421L3 | Lenti ORF clone of Human actin filament associated protein 1 (AFAP1), transcript variant 1, Myc-DDK-tagged |
CNY 9,220.00 |
|
| RC207421L4 | Lenti ORF clone of Human actin filament associated protein 1 (AFAP1), transcript variant 1, mGFP tagged |
CNY 9,220.00 |
|
| SC321715 | AFAP1 (untagged)-Human actin filament associated protein 1 (AFAP1), transcript variant 1 |
CNY 5,352.00 |
