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RAIN (RASIP1) (NM_017805) Human Tagged ORF Clone
CAT＃: RG206189
- TrueORF®
RASIP1 (tGFP-tagged) - Human Ras interacting protein 1 (RASIP1)
ORF Plasmid: DDK
"NM_017805" in other vectors (6)
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CNY 4,370.00
Product images
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | RAIN |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG206189 representing NM_017805
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCTGTCTGGTGAACGGAAGGAGGGCGGAAGCCCCCGCTTCGGGAAGCTTCATCTCCCCGTGGGCCTGT GGATCAATTCCCCCAGGAAGCAGCTGGCGAAGCTGGGGCGGCGCTGGCCCAGCGCAGCCTCTGTCAAGTC TTCTTCGTCGGACACGGGGAGCCGCAGCAGCGAGCCGCTACCTCCGCCCCCGCCGCACGTGGAGCTGCGG CGAGTGGGCGCTGTCAAGGCGGCCGGGGGAGCCTCCGGTAGCCGCGCCAAGCGCATCTCCCAGCTCTTCC GGGGCTCGGGGACCGGGACCACGGGGTCCAGCGGCGCAGGAGGCCCTGGGACCCCGGGGGGCGCGCAGCG CTGGGCCAGCGAGAAGAAGCTGCCGGAGCTGGCGGCGGGCGTGGCCCCCGAGCCCCCGCTGGCTACCCGC GCCACGGCGCCTCCGGGGGTCCTCAAGATCTTCGGCGCCGGACTGGCATCGGGCGCCAACTACAAGAGCG TGCTGGCCACGGCGCGCTCCACGGCGCGCGAGCTCGTGGCCGAGGCGCTAGAGCGCTACGGCCTAGCAGG CAGCCCCGGCGGTGGCCCCGGCGAGAGCAGCTGCGTGGACGCCTTCGCTTTGTGCGACGCTCTGGGCCGG CCCGCGGCGGCGGGCGTGGGAAGCGGCGAGTGGCGGGCGGAGCACCTGCGCGTGCTGGGCGACTCCGAGC GCCCGCTGCTGGTGCAGGAGCTGTGGCGGGCGCGGCCCGGCTGGGCGCGGCGCTTCGAGTTGCGCGGCCG CGAGGAGGCGCGGCGCCTGGAGCAGGAGGCCTTCGGGGCCGCGGACAGCGAAGGCACCGGCGCCCCTTCG TGGCGGCCACAGAAGAACCGCTCCCGGGCGGCGTCGGGTGGGGCAGCGCTGGCCAGTCCTGGCCCGGGGA CCGGATCAGGGGCCCCAGCTGGGTCTGGAGGCAAGGAGCGCTCAGAAAACTTGTCTTTGCGGCGCAGCGT GTCGGAGCTTAGCCTTCAGGGGCGGCGGCGGCGGCAGCAGGAGCGGAGACAGCAGGCACTTAGCATGGCC CCAGGGGCAGCCGACGCCCAAATCGGAACTGCAGACCCCGGGGACTTCGATCAGTTGACTCAGTGCCTCA TCCAGGCCCCCAGCAACCGCCCCTACTTCCTGCTGCTCCAGGGCTACCAGGACGCCCAGGACTTTGTGGT GTATGTGATGACGCGAGAGCAGCACGTGTTTGGGCGAGGTGGGAACTCGTCTGGCCGCGGGGGGTCCCCG GCTCCCTATGTGGACACCTTCCTCAACGCCCCGGACATCCTGCCGCGTCACTGCACAGTGCGCGCGGGCC CTGAGCACCCGGCCATGGTGCGCCCGTCCCGGGGCGCCCCAGTCACGCACAACGGGTGCCTCCTGCTGCG GGAGGCTGAGCTGCACCCGGGCGACCTCCTGGGGCTGGGCGAGCACTTCCTGTTCATGTACAAGGACCCC CGCACTGGGGGCTCGGGGCCTGCGAGGCCGCCGTGGCTGCCCGCGCGCCCCGGGGCCACGCCGCCAGGCC CTGGCTGGGCCTTCTCCTGTCGCCTGTGCGGCCGCGGCCTGCAGGAGCGCGGCGAGGCACTGGCCGCCTA CCTGGACGGCCGTGAGCCAGTCCTGCGCTTCCGGCCGCGCGAGGAGGAGGCGCTGCTGGGCGAGATCGTG CGCGCCGCAGCCGCCGGCTCGGGAGACCTGCCGCCCCTCGGGCCCGCCACGCTGCTGGCGCTGTGCGTGC AGCATTCCGCCCGTGAGCTGGAGCTGGGCCACCTGCCACGACTGCTGGGCCGCCTGGCCCGGCTCATCAA GGAGGCCGTCTGGGAAAAGATTAAGGAAATTGGAGACCGTCAGCCAGAAAACCACCCTGAGGGGGTCCCC GAGGTGCCCCTGACTCCTGAAGCTGTGTCTGTGGAGCTGCGGCCACTCATGCTGTGGATGGCCAACACCA CGGAGCTGCTTAGCTTTGTGCAGGAGAAGGTGCTGGAAATGGAGAAGGAGGCTGACCAAGAGGACCCACA GCTCTGCAATGACTTGGAATTATGTGATGAGGCCATGGCCCTCCTGGATGAGGTCATCATGTGTACCTTC CAGCAGTCTGTCTACTACCTCACCAAGACTCTCTATTCAACGCTGCCTGCTCTCCTGGATAGTAACCCTT TCACAGCTGGTGCAGAGCTGCCGGGGCCTGGCGCGGAGCTGGGGGCAATGCCTCCAGGATTGAGACCTAC CCTGGGCGTGTTCCAGGCAGCCTTGGAGCTGACCAGCCAGTGCGAGCTGCACCCTGACCTCGTGTCTCAG ACTTTTGGCTACTTGTTCTTCTTCTCCAACGCATCCCTTCTCAACTCGCTGATGGAACGAGGTCAAGGCC GGCCTTTCTATCAATGGTCCCGAGCTGTTCAAATCCGAACCAACCTGGACCTCGTCTTGGACTGGCTACA GGGAGCTGGGCTGGGCGACATTGCCACTGAGTTCTTCCGGAAACTCTCCATGGCTGTGAACCTGCTCTGT GTGCCCCGCACTTCCCTGCTCAAGGCTTCATGGAGCAGCCTAAGAACCGACCACCCCACCTTGACCCCCG CCCAGCTGCACCATCTGCTCAGCCACTATCAGCTGGGCCCTGGCCGCGGGCCGCCAGCCGCGTGGGACCC TCCCCCTGCAGAGCGGGAGGCTGTGGACACAGGGGACATCTTCGAAAGCTTCTCCTCGCACCCGCCCCTC ATCCTCCCCCTGGGGAGCTCGCGCCTGCGCCTCACTGGTCCAGTGACGGACGATGCCTTGCACCGTGAAC TCCGTAGGCTCCGCCGCCTCCTCTGGGATCTTGAGCAGCAGGAGCTGCCAGCCAATTATCGCCATGGGCC TCCCGTGGCCACGTCTCCT ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG206189 representing NM_017805
Red=Cloning site Green=Tags(s) MLSGERKEGGSPRFGKLHLPVGLWINSPRKQLAKLGRRWPSAASVKSSSSDTGSRSSEPLPPPPPHVELR RVGAVKAAGGASGSRAKRISQLFRGSGTGTTGSSGAGGPGTPGGAQRWASEKKLPELAAGVAPEPPLATR ATAPPGVLKIFGAGLASGANYKSVLATARSTARELVAEALERYGLAGSPGGGPGESSCVDAFALCDALGR PAAAGVGSGEWRAEHLRVLGDSERPLLVQELWRARPGWARRFELRGREEARRLEQEAFGAADSEGTGAPS WRPQKNRSRAASGGAALASPGPGTGSGAPAGSGGKERSENLSLRRSVSELSLQGRRRRQQERRQQALSMA PGAADAQIGTADPGDFDQLTQCLIQAPSNRPYFLLLQGYQDAQDFVVYVMTREQHVFGRGGNSSGRGGSP APYVDTFLNAPDILPRHCTVRAGPEHPAMVRPSRGAPVTHNGCLLLREAELHPGDLLGLGEHFLFMYKDP RTGGSGPARPPWLPARPGATPPGPGWAFSCRLCGRGLQERGEALAAYLDGREPVLRFRPREEEALLGEIV RAAAAGSGDLPPLGPATLLALCVQHSARELELGHLPRLLGRLARLIKEAVWEKIKEIGDRQPENHPEGVP EVPLTPEAVSVELRPLMLWMANTTELLSFVQEKVLEMEKEADQEDPQLCNDLELCDEAMALLDEVIMCTF QQSVYYLTKTLYSTLPALLDSNPFTAGAELPGPGAELGAMPPGLRPTLGVFQAALELTSQCELHPDLVSQ TFGYLFFFSNASLLNSLMERGQGRPFYQWSRAVQIRTNLDLVLDWLQGAGLGDIATEFFRKLSMAVNLLC VPRTSLLKASWSSLRTDHPTLTPAQLHHLLSHYQLGPGRGPPAAWDPPPAEREAVDTGDIFESFSSHPPL ILPLGSSRLRLTGPVTDDALHRELRRLRRLLWDLEQQELPANYRHGPPVATSP TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_017805 |
| ORF Size | 2889 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_017805.2, NP_060275.2 |
| RefSeq Size | 3311 bp |
| RefSeq ORF | 2892 bp |
| Locus ID | 54922 |
| UniProt ID | Q5U651 |
| Domains | DIL |
| Gene Summary | Required for the proper formation of vascular structures that develop via both vasculogenesis and angiogenesis. Acts as a critical and vascular-specific regulator of GTPase signaling, cell architecture, and adhesion, which is essential for endothelial cell morphogenesis and blood vessel tubulogenesis. Regulates the activity of Rho GTPases in part by recruiting ARHGAP29 and suppressing RhoA signaling and dampening ROCK and MYH9 activities in endothelial cells (By similarity). May act as effector for Golgi-bound HRAS and other Ras-like proteins. May promote HRAS-mediated transformation. Negative regulator of amino acid starvation-induced autophagy.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC206189 | RASIP1 (Myc-DDK-tagged)-Human Ras interacting protein 1 (RASIP1) |
CNY 3,990.00 |
|
| RC206189L1 | Lenti ORF clone of Human Ras interacting protein 1 (RASIP1), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC206189L2 | Lenti ORF clone of Human Ras interacting protein 1 (RASIP1), mGFP tagged |
CNY 5,890.00 |
|
| RC206189L3 | Lenti ORF clone of Human Ras interacting protein 1 (RASIP1), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC206189L4 | Lenti ORF clone of Human Ras interacting protein 1 (RASIP1), mGFP tagged |
CNY 5,890.00 |
|
| SC113959 | RASIP1 (untagged)-Human Ras interacting protein 1 (RASIP1) |
CNY 7,220.00 |
