MAGEB4 (NM_002367) Human Tagged ORF Clone

CAT#: RG206060

  • TrueORF®

MAGEB4 (tGFP-tagged) - Human melanoma antigen family B, 4 (MAGEB4)

ORF Plasmid: DDK tGFP



  "NM_002367" in other vectors (4)

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CNY 5,256.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (5)
pCMV6-AC-GFP, mammalian vector with C-terminal tGFP tag, 10ug
    • 10 ug

CNY 6,080.00


Mouse monoclonal turboGFP antibody, clone OTI2H8
    • 100 ul

CNY 1,000.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


MAGEB4 mouse monoclonal antibody,clone OTI1B5
    • 100 ul

CNY 1,999.00
CNY 2,700.00

Specifications

Product Data
Type Human Tagged ORF Clone
Tag TurboGFP
Synonyms CT3.6
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RG206060 representing NM_002367
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCCTCGGGGTCAGAAGAGTAAGCTCCGTGCCCGTGAGAAACGCCAGCGGACCCGTGGTCAGACCCAGG
ATCTCAAGGTTGGTCAGCCTACTGCAGCAGAGAAAGAAGAGTCTCCTTCCCCTTCCTCATCTGTTTTGAG
GGATACTGCCTCCAGCTCCCTTGCTTTTGGCATTCCCCAGGAGCCTCAGAGAGAGCCACCCACCACCTCT
GCTGCTGCAGCTATGTCATGCACTGGATCTGATAAAGGCGACGAGAGCCAAGATGAGGAAAATGCAAGTT
CCTCCCAGGCCTCAACATCCACTGAGAGATCACTCAAAGATTCTCTAACCAGGAAGACGAAGATGTTAGT
GCAGTTCCTGCTGTACAAGTATAAAATGAAAGAGCCCACTACAAAGGCAGAAATGCTGAAGATCATCAGC
AAAAAGTACAAGGAGCACTTCCCTGAGATCTTCAGGAAAGTCTCTCAGCGCACGGAGCTGGTCTTTGGCC
TTGCCTTGAAGGAGGTCAACCCCACCACTCACTCCTACATCCTCGTCAGCATGCTAGGCCCCAACTATGG
AAACCAGAGCAGTGCCTGGACCCTTCCAAGGAATGGGCTTCTGATGCCTCTACTGAGTGTGATCTTCTTA
AATGGCAACTGTGCCCGTGAAGAGGAAATCTGGGAATTCCTGAATATGCTGGGGATCTATGATGGAAAGA
GGCACCTTATCTTTGGGGAACCCCGAAAGCTCATCACCCAAGATCTGGTGCAGGAAAAATATCTGGAATA
CCAGCAGGTGCCCAACAGTGATCCCCCACGCTATCAATTCCTGTGGGGTCCAAGAGCTCATGCAGAAACC
AGCAAGATGAAAGTCCTGGAGTTTTTGGCCAAGGTGAATGACACCACCCCCAATAACTTCCCACTCCTTT
ATGAAGAGGCTTTGAGAGATGAAGAAGAGAGAGCTGGAGCCCGGCCCAGAGTTGCAGCCAGGCGTGGCAC
TACAGCCATGACTAGTGCGTATTCCAGGGCCACATCCAGTAGCTCTTCCCAACCCATG


ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
>RG206060 representing NM_002367
Red=Cloning site Green=Tags(s)

MPRGQKSKLRAREKRQRTRGQTQDLKVGQPTAAEKEESPSPSSSVLRDTASSSLAFGIPQEPQREPPTTS
AAAAMSCTGSDKGDESQDEENASSSQASTSTERSLKDSLTRKTKMLVQFLLYKYKMKEPTTKAEMLKIIS
KKYKEHFPEIFRKVSQRTELVFGLALKEVNPTTHSYILVSMLGPNYGNQSSAWTLPRNGLLMPLLSVIFL
NGNCAREEEIWEFLNMLGIYDGKRHLIFGEPRKLITQDLVQEKYLEYQQVPNSDPPRYQFLWGPRAHAET
SKMKVLEFLAKVNDTTPNNFPLLYEEALRDEEERAGARPRVAARRGTTAMTSAYSRATSSSSSQPM

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_002367
ORF Size 1038 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_002367.2, NP_002358.1
RefSeq Size 2142 bp
RefSeq ORF 1041 bp
Locus ID 4115
UniProt ID O15481
Gene Summary This gene is a member of the MAGEB gene family. The members of this family have their entire coding sequences located in the last exon, and the encoded proteins show 50 to 68% sequence identity to each other. The promoters and first exons of the MAGEB genes show considerable variability, suggesting that the existence of this gene family enables the same function to be expressed under different transcriptional controls. The MAGEB genes are clustered on chromosome Xp22-p21. This gene sequence ends in the first intron of MAGEB1, another family member. This gene is expressed in testis. [provided by RefSeq, Jul 2008]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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