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GNPTG (NM_032520) Human Tagged ORF Clone
CAT＃: RG205280
- TrueORF®
GNPTG (tGFP-tagged) - Human N-acetylglucosamine-1-phosphate transferase, gamma subunit (GNPTG)
ORF Plasmid: DDK
"NM_032520" in other vectors (6)
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CNY 4,000.00
CNY 4,370.00
Product images
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | C16orf27; GNPTAG; LP2537; RJD9 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG205280 representing NM_032520
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGGCGGGGCTGGCGCGGCTCCTGTTGCTCCTCGGGCTCTCGGCCGGCGGGCCCGCGCCGGCAGGTG CAGCGAAGATGAAGGTGGTGGAGGAGCCCAACGCGTTTGGGGTGAACAACCCGTTCTTGCCTCAGGCCAG TCGCCTCCAGGCCAAGAGGGATCCTTCACCCGTGTCTGGACCCGTGCATCTCTTCCGACTCTCGGGCAAG TGCTTCAGCCTGGTGGAGTCCACGTACAAGTATGAGTTCTGCCCGTTCCACAACGTGACCCAGCACGAGC AGACCTTCCGCTGGAACGCCTACAGTGGGATCCTCGGCATCTGGCACGAGTGGGAGATCGCCAACAACAC CTTCACGGGCATGTGGATGAGGGACGGTGACGCCTGCCGTTCCCGGAGCCGGCAGAGCAAGGTGGAGCTG GCGTGTGGAAAAAGCAACCGGCTGGCCCATGTGTCCGAGCCGAGCACCTGCGTCTACGCGCTGACGTTCG AGACCCCCCTCGTCTGCCACCCCCACGCCTTGCTAGTGTACCCAACCCTGCCAGAGGCCCTGCAGCGGCA GTGGGACCAGGTAGAGCAGGACCTGGCCGATGAGCTGATCACCCCCCAGGGCCATGAGAAGTTGCTGAGG ACACTTTTTGAGGATGCTGGCTACTTAAAGACCCCAGAAAATGAACCCACCCAGCTGGAGGGAGGTCCTG ACAGCTTGGGGTTTGAGACCCTGGAAAACTGCAGGAAGGCTCATAAAGAACTCTCAAAGGAGATCAAAAG GCTGAAAGGTTTGCTCACCCAGCACGGCATCCCCTACACGAGGCCCACAGAAACTTCCAACTTGGAGCAC TTGGGCCACGAGACGCCCAGAGCCAAGTCTCCAGAGCAGCTGCGGGGTGACCCAGGACTGCGTGGGAGTT TG AGCGGACCGACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG205280 representing NM_032520
Red=Cloning site Green=Tags(s) MAAGLARLLLLLGLSAGGPAPAGAAKMKVVEEPNAFGVNNPFLPQASRLQAKRDPSPVSGPVHLFRLSGK CFSLVESTYKYEFCPFHNVTQHEQTFRWNAYSGILGIWHEWEIANNTFTGMWMRDGDACRSRSRQSKVEL ACGKSNRLAHVSEPSTCVYALTFETPLVCHPHALLVYPTLPEALQRQWDQVEQDLADELITPQGHEKLLR TLFEDAGYLKTPENEPTQLEGGPDSLGFETLENCRKAHKELSKEIKRLKGLLTQHGIPYTRPTETSNLEH LGHETPRAKSPEQLRGDPGLRGSL SGPTRTRRLE - GFP Tag - V |
| Restriction Sites |
SgfI-RsrII
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_032520 |
| ORF Size | 912 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_032520.3, NP_115909.1 |
| RefSeq Size | 1228 bp |
| RefSeq ORF | 918 bp |
| Locus ID | 84572 |
| UniProt ID | Q9UJJ9 |
| Protein Families | Secreted Protein |
| Gene Summary | This gene encodes the gamma sunbunit of the N-acetylglucosamine-1-phosphotransferase complex. This hexameric complex, composed of alpha, beta and gamma subunits, catalyzes the first step in synthesis of a mannose 6-phosphate lysosomal recognition marker. This enzyme complex is necessary for targeting of lysosomal hydrolases to the lysosome. Mutations in the gene encoding the gamma subunit have been associated with mucolipidosis IIIC, also known as mucolipidosis III gamma.[provided by RefSeq, Feb 2010] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC205280 | GNPTG (Myc-DDK-tagged)-Human N-acetylglucosamine-1-phosphate transferase, gamma subunit (GNPTG) |
CNY 2,400.00 |
|
| RC205280L1 | Lenti-ORF clone of GNPTG (Myc-DDK-tagged)-Human N-acetylglucosamine-1-phosphate transferase, gamma subunit (GNPTG) |
CNY 4,800.00 |
|
| RC205280L2 | Lenti-ORF clone of GNPTG (mGFP-tagged)-Human N-acetylglucosamine-1-phosphate transferase, gamma subunit (GNPTG) |
CNY 5,890.00 |
|
| RC205280L3 | Lenti-ORF clone of GNPTG (Myc-DDK-tagged)-Human N-acetylglucosamine-1-phosphate transferase, gamma subunit (GNPTG) |
CNY 5,890.00 |
|
| RC205280L4 | Lenti-ORF clone of GNPTG (mGFP-tagged)-Human N-acetylglucosamine-1-phosphate transferase, gamma subunit (GNPTG) |
CNY 5,890.00 |
|
| SC108015 | GNPTG (untagged)-Human N-acetylglucosamine-1-phosphate transferase, gamma subunit (GNPTG) |
CNY 6,270.00 |
