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MMD (NM_012329) Human Tagged ORF Clone
CAT＃: RG205241
- TrueORF®
MMD (tGFP-tagged) - Human monocyte to macrophage differentiation-associated (MMD)
ORF Plasmid: DDK
"NM_012329" in other vectors (4)
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CNY 4,000.00
CNY 4,370.00
Product images
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | MMA; MMD1; PAQR11 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG205241 representing NM_012329
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCGGTTCAAGAATCGATTCCAGCGGTTCATGAACCATCGAGCTCCAGCCAATGGCCGCTACAAGCCAA CTTGCTATGAACATGCTGCTAACTGTTACACACACGCATTCCTCATTGTTCCGGCCATCGTGGGCAGTGC CCTCCTCCATCGGCTGTCTGATGACTGCTGGGAAAAGATAACAGCATGGATTTATGGAATGGGACTCTGT GCCCTCTTCATCGTTTCTACAGTATTTCACATTGTATCATGGAAAAAGAGCCACTTAAGGACAGTGGAGC ATTGTTTTCACATGTGTGATAGAATGGTTATCTATTTCTTCATTGCTGCTTCTTATGCTCCATGGTTAAA TCTTCGTGAACTTGGACCCCTGGCATCTCATATGCGTTGGTTTATCTGGCTCATGGCAGCTGGAGGAACC ATTTATGTATTTCTCTACCATGAAAAATATAAGGTGGTTGAACTCTTTTTCTATCTCACAATGGGATTCT CTCCAGCCTTGGTGGTGACATCAATGAACAACACCGATGGACTTCAGGAACTTGCCTGTGGGGGCTTAAT TTATTGCTTGGGAGTTGTGTTCTTCAAGAGTGATGGCATCATTCCATTTGCCCACGCCATCTGGCACCTG TTTGTGGCCACGGCAGCTGCAGTGCATTACTACGCCATTTGGAAATACCTTTACCGAAGTCCTACGGACT TTATGCGGCATTTA ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG205241 representing NM_012329
Red=Cloning site Green=Tags(s) MRFKNRFQRFMNHRAPANGRYKPTCYEHAANCYTHAFLIVPAIVGSALLHRLSDDCWEKITAWIYGMGLC ALFIVSTVFHIVSWKKSHLRTVEHCFHMCDRMVIYFFIAASYAPWLNLRELGPLASHMRWFIWLMAAGGT IYVFLYHEKYKVVELFFYLTMGFSPALVVTSMNNTDGLQELACGGLIYCLGVVFFKSDGIIPFAHAIWHL FVATAAAVHYYAIWKYLYRSPTDFMRHL TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_012329 |
| ORF Size | 714 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_012329.3 |
| RefSeq Size | 2723 bp |
| RefSeq ORF | 717 bp |
| Locus ID | 23531 |
| UniProt ID | Q15546 |
| Domains | UPF0073 |
| Protein Families | Druggable Genome, Ion Channels: Other, Transmembrane |
| Gene Summary | This protein is expressed by in vitro differentiated macrophages but not freshly isolated monocytes. Although sequence analysis identifies seven potential transmembrane domains, this protein has little homology to G-protein receptors and it has not been positively identified as a receptor. A suggested alternative function is that of an ion channel protein in maturing macrophages. [provided by RefSeq, Jul 2008] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC205241 | MMD (Myc-DDK-tagged)-Human monocyte to macrophage differentiation-associated (MMD) |
CNY 2,400.00 |
|
| RC205241L3 | Lenti ORF clone of Human monocyte to macrophage differentiation-associated (MMD), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC205241L4 | Lenti ORF clone of Human monocyte to macrophage differentiation-associated (MMD), mGFP tagged |
CNY 5,890.00 |
|
| SC111208 | MMD (untagged)-Human monocyte to macrophage differentiation-associated (MMD) |
CNY 2,400.00 |
