ARSF (NM_004042) Human Tagged ORF Clone
CAT#: RG205058
- TrueORF®
ARSF (tGFP-tagged) - Human arylsulfatase F (ARSF), transcript variant 1
ORF Plasmid: DDK
"NM_004042" in other vectors (7)
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CNY 5,992.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | TurboGFP |
Synonyms | ASF |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RG205058 representing NM_004042
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGGCCCAGGAGACCCTTGGTCTTCATGTCTTTGGTGTGTGCACTCTTGAACACATGCCAGGCACACA GGGTGCATGACGACAAGCCTAATATTGTCCTAATCATGGTTGATGACCTGGGTATTGGAGATCTGGGCTG CTACGGCAATGACACCATGAGGACGCCTCACATCGACCGCCTTGCCAGGGAAGGCGTGCGACTGACTCAG CACATCTCTGCCGCCTCCCTCTGCAGCCCAAGCCGGTCCGCGTTCTTGACGGGAAGATACCCCATCCGAT CAGGTATGGTTTCTAGTGGTAATAGACGTGTCATCCAAAATCTTGCAGTCCCCGCAGGCCTCCCTCTTAA TGAGACAACACTTGCAGCCTTGCTAAAGAAGCAAGGATACAGCACGGGGCTTATAGGCAAATGGCACCAA GGCTTGAACTGCGACTCCCGAAGTGACCAGTGCCACCATCCATATAATTATGGGTTTGACTACTACTATG GCATGCCGTTCACTCTCGTTGACAGCTGCTGGCCGGACCCCTCTCGTAACACGGAATTAGCCTTTGAGAG TCAGCTCTGGCTCTGTGTGCAGCTAGTTGCCATTGCCATCCTCACCCTAACCTTTGGGAAGCTGAGCGGC TGGGTCTCTGTTCCCTGGCTCCTGATCTTCTCCATGATTCTGTTTATTTTCCTCTTGGGCTATGCTTGGT TCTCCAGCCACACGTCCCCTTTATACTGGGACTGCCTCCTCATGCGGGGGCACGAGATCACGGAGCAGCC CATGAAGGCTGAACGAGCTGGATCCATTATGGTGAAGGAAGCGATTTCCTTTTTAGAAAGGCACAGTAAG GAAACTTTCCTTCTCTTTTTCTCCTTTCTTCACGTGCACACACCTCTCCCCACCACGGACGATTTCACTG GCACCAGCAAGCATGGCTTGTATGGGGATAATGTGGAAGAGATGGACTCCATGGTGGGCAAGATTCTTGA TGCTATCGATGATTTTGGCCTAAGGAACAACACCCTTGTCTACTTTACATCAGATCACGGAGGGCATTTG GAAGCTAGGCGAGGGCATGCCCAACTTGGTGGATGGAATGGAATATACAAAGGTGGAAAAGGCATGGGGG GCTGGGAAGGTGGAATCCGCGTCCCAGGAATTGTCCGATGGCCTGGAAAGGTACCAGCTGGACGGTTGAT TAAGGAACCTACAAGTTTAATGGATATTTTACCAACTGTCGCATCAGTGTCAGGAGGAAGTCTCCCTCAG GACAGGGTCATTGACGGCCGAGACCTCATGCCCTTGCTGCAGGGCAACGTCAGGCACTCGGAGCATGAAT TTCTTTTCCACTACTGTGGCTCCTACCTGCACGCCGTGCGGTGGATCCCCAAGGACGACAGTGGGTCAGT TTGGAAGGCTCACTATGTGACCCCGGTATTCCAGCCACCAGCTTCTGGTGGCTGCTATGTCACCTCATTA TGCAGATGTTTCGGAGAACAGGTTACCTACCACAACCCCCCTCTGCTCTTCGATCTCTCCAGGGACCCCT CAGAGTCCACACCCCTGACACCTGCCACAGAGCCCCTCTATGATTTTGTGATTAAAAAGGTGGCCAACGC CCTGAAGGAACACCAGGAAACCATCGTGCCTGTGACCTACCAACTCTCAGAACTGAATCAGGGCAGGACG TGGCTGAAGCCTTGCTGTGGGGTGTTCCCATTTTGTCTGTGTGACAAGGAAGAGGAAGTCTCTCAGCCTC GGGGTCCTAACGAGAAGAGA ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG205058 representing NM_004042
Red=Cloning site Green=Tags(s) MRPRRPLVFMSLVCALLNTCQAHRVHDDKPNIVLIMVDDLGIGDLGCYGNDTMRTPHIDRLAREGVRLTQ HISAASLCSPSRSAFLTGRYPIRSGMVSSGNRRVIQNLAVPAGLPLNETTLAALLKKQGYSTGLIGKWHQ GLNCDSRSDQCHHPYNYGFDYYYGMPFTLVDSCWPDPSRNTELAFESQLWLCVQLVAIAILTLTFGKLSG WVSVPWLLIFSMILFIFLLGYAWFSSHTSPLYWDCLLMRGHEITEQPMKAERAGSIMVKEAISFLERHSK ETFLLFFSFLHVHTPLPTTDDFTGTSKHGLYGDNVEEMDSMVGKILDAIDDFGLRNNTLVYFTSDHGGHL EARRGHAQLGGWNGIYKGGKGMGGWEGGIRVPGIVRWPGKVPAGRLIKEPTSLMDILPTVASVSGGSLPQ DRVIDGRDLMPLLQGNVRHSEHEFLFHYCGSYLHAVRWIPKDDSGSVWKAHYVTPVFQPPASGGCYVTSL CRCFGEQVTYHNPPLLFDLSRDPSESTPLTPATEPLYDFVIKKVANALKEHQETIVPVTYQLSELNQGRT WLKPCCGVFPFCLCDKEEEVSQPRGPNEKR TRTRPLE - GFP Tag - V |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_004042 |
ORF Size | 1770 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_004042.3, NP_004033.2 |
RefSeq Size | 2012 bp |
RefSeq ORF | 1773 bp |
Locus ID | 416 |
UniProt ID | P54793 |
Domains | Sulfatase |
Protein Families | Druggable Genome, Secreted Protein, Transmembrane |
Gene Summary | This gene is a member of the sulfatase family, and more specifically, the arylsulfatase subfamily. Members of the subfamily share similarity in sequence and splice sites, and are clustered together on chromosome X, suggesting that they are derived from recent gene duplication events. Sulfatases are essential for the correct composition of bone and cartilage matrix. The activity of this protein, unlike that of arylsulfatase E, is not inhibited by warfarin. Multiple alternatively spliced variants, encoding the same protein, have been identified.[provided by RefSeq, Jan 2011] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC205058 | ARSF (Myc-DDK-tagged)-Human arylsulfatase F (ARSF), transcript variant 1 |
CNY 4,392.00 |
|
RC205058L1 | Lenti ORF clone of Human arylsulfatase F (ARSF), transcript variant 1, Myc-DDK-tagged |
CNY 6,792.00 |
|
RC205058L2 | Lenti ORF clone of Human arylsulfatase F (ARSF), transcript variant 1, mGFP tagged |
CNY 5,890.00 |
|
RC205058L3 | Lenti ORF clone of Human arylsulfatase F (ARSF), transcript variant 1, Myc-DDK-tagged |
CNY 5,890.00 |
|
RC205058L4 | Lenti ORF clone of Human arylsulfatase F (ARSF), transcript variant 1, mGFP tagged |
CNY 5,890.00 |
|
SC117626 | ARSF (untagged)-Human arylsulfatase F (ARSF), transcript variant 1 |
CNY 4,400.00 |
|
SC320958 | ARSF (untagged)-Human arylsulfatase F (ARSF), transcript variant 1 |
CNY 4,400.00 |