TFB1M (NM_016020) Human Tagged ORF Clone
CAT#: RG204643
- TrueORF®
TFB1M (tGFP-tagged) - Human transcription factor B1, mitochondrial (TFB1M), nuclear gene encoding mitochondrial protein
ORF Plasmid: DDK
"NM_016020" in other vectors (7)
Need custom modification / cloning service?
Get a free quote
CNY 5,256.00
CNY 1,999.00
CNY 2,700.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | TurboGFP |
Synonyms | CGI-75; CGI75; mtTFB; mtTFB1 |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RG204643 representing NM_016020
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCTGCCTCCGGAAAACTCAGCACTTGCCGTCTCCCTCCGTTGCCCACGATTCGAGAAATCATTAAGT TGTTAAGACTGCAAGCAGCGAAGCAGCTATCACAGAATTTCCTCCTGGACTTGAGGCTGACAGATAAGAT TGTAAGGAAAGCTGGCAATCTGACAAATGCTTATGTTTACGAAGTGGGCCCTGGGCCAGGGGGAATCACA AGATCTATTCTTAATGCCGACGTCGCTGAACTTCTGGTGGTTGAAAAGGACACTCGATTTATTCCTGGAT TACAGATGCTTTCTGATGCAGCACCTGGGAAACTGAGAATTGTTCATGGAGATGTCTTGACATTTAAGGT AGAAAAGGCTTTTTCAGAAAGTCTTAAAAGACCCTGGGAAGATGATCCTCCAAATGTACATATTATTGGA AATCTGCCTTTTAGTGTTTCAACTCCACTGATTATCAAGTGGCTTGAAAATATTTCCTGTAGAGATGGAC CTTTTGTTTATGGCAGAACTCAGATGACTTTGACTTTTCAAAAGGAAGTGGCAGAGAGACTTGCAGCCAA TACAGGAAGCAAACAGCGTAGTCGCCTCTCTGTTATGGCTCAGTACCTCTGCAATGTTCGACACATCTTT ACGATTCCAGGACAAGCTTTTGTCCCCAAACCAGAGGTGGACGTGGGCGTGGTGCACTTCACTCCCTTGA TACAGCCCAAGATAGAGCAGCCATTCAAGCTGGTGGAAAAAGTGGTTCAGAATGTATTTCAGTTCCGAAG GAAATACTGCCATCGAGGGCTCAGAATGTTATTCCCTGAAGCGCAGCGCTTGGAAAGCACGGGCAGGCTG TTAGAGTTGGCAGACATAGACCCTACTCTTCGGCCCCGCCAGCTCTCCATCTCACACTTTAAGAGCCTCT GTGATGTATACAGAAAAATGTGTGATGAAGACCCACAACTCTTTGCATATAATTTCAGAGAAGAACTCAA GCGAAGAAAAAGCAAAAATGAAGAAAAAGAAGAGGATGACGCAGAGAATTACAGACTC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG204643 representing NM_016020
Red=Cloning site Green=Tags(s) MAASGKLSTCRLPPLPTIREIIKLLRLQAAKQLSQNFLLDLRLTDKIVRKAGNLTNAYVYEVGPGPGGIT RSILNADVAELLVVEKDTRFIPGLQMLSDAAPGKLRIVHGDVLTFKVEKAFSESLKRPWEDDPPNVHIIG NLPFSVSTPLIIKWLENISCRDGPFVYGRTQMTLTFQKEVAERLAANTGSKQRSRLSVMAQYLCNVRHIF TIPGQAFVPKPEVDVGVVHFTPLIQPKIEQPFKLVEKVVQNVFQFRRKYCHRGLRMLFPEAQRLESTGRL LELADIDPTLRPRQLSISHFKSLCDVYRKMCDEDPQLFAYNFREELKRRKSKNEEKEEDDAENYRL TRTRPLE - GFP Tag - V |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
![]() |
ACCN | NM_016020 |
ORF Size | 1038 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_016020.4 |
RefSeq Size | 1290 bp |
RefSeq ORF | 1041 bp |
Locus ID | 51106 |
UniProt ID | Q8WVM0 |
Domains | rADc |
Protein Families | Transcription Factors |
Gene Summary | The protein encoded by this gene is a dimethyltransferase that methylates the conserved stem loop of mitochondrial 12S rRNA. The encoded protein also is part of the basal mitochondrial transcription complex and is necessary for mitochondrial gene expression. The methylation and transcriptional activities of this protein are independent of one another. Variations in this gene may influence the severity of aminoglycoside-induced deafness (AID).[provided by RefSeq, Aug 2010] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC204643 | TFB1M (Myc-DDK-tagged)-Human transcription factor B1, mitochondrial (TFB1M), nuclear gene encoding mitochondrial protein |
CNY 3,656.00 |
|
RC204643L1 | Lenti ORF clone of Human transcription factor B1, mitochondrial (TFB1M), nuclear gene encoding mitochondrial protein, Myc-DDK-tagged |
CNY 6,056.00 |
|
RC204643L2 | Lenti ORF clone of Human transcription factor B1, mitochondrial (TFB1M), nuclear gene encoding mitochondrial protein, mGFP tagged |
CNY 5,890.00 |
|
RC204643L3 | Lenti ORF clone of Human transcription factor B1, mitochondrial (TFB1M), nuclear gene encoding mitochondrial protein, Myc-DDK-tagged |
CNY 5,890.00 |
|
RC204643L4 | Lenti ORF clone of Human transcription factor B1, mitochondrial (TFB1M), nuclear gene encoding mitochondrial protein, mGFP tagged |
CNY 5,890.00 |
|
SC311121 | TFB1M (untagged)-Human transcription factor B1, mitochondrial (TFB1M), nuclear gene encoding mitochondrial protein |
CNY 3,656.00 |
|
SC324517 | TFB1M (untagged)-Human transcription factor B1, mitochondrial (TFB1M), nuclear gene encoding mitochondrial protein |
CNY 3,656.00 |