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DRAM (DRAM1) (NM_018370) Human Tagged ORF Clone
CAT＃: RG204574
- TrueORF®
DRAM1 (tGFP-tagged) - Human DNA-damage regulated autophagy modulator 1 (DRAM1)
ORF Plasmid: DDK
"NM_018370" in other vectors (5)
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CNY 4,000.00
CNY 4,370.00
Product images
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | DRAM |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG204574 representing NM_018370
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCTGTGCTTCCTGAGGGGAATGGCTTTCGTCCCCTTCCTCTTGGTGACCTGGTCGTCAGCCGCCTTCA TTATCTCCTACGTGGTCGCCGTGCTCTCCGGGCACGTCAACCCCTTCCTCCCGTATATCAGTGATACGGG AACAACACCTCCAGAGAGTGGTATTTTTGGATTTATGATAAACTTCTCTGCATTTCTTGGTGCAGCCACG ATGTATACAAGATACAAAATAGTACAGAAGCAAAATCAAACCTGCTATTTCAGCACTCCTGTTTTTAACT TGGTGTCTTTAGTGCTTGGATTGGTGGGATGTTTCGGAATGGGCATTGTCGCCAATTTTCAGGAGTTAGC TGTGCCAGTGGTTCATGACGGGGGCGCTCTTTTGGCCTTTGTCTGTGGTGTCGTGTACACGCTCCTACAG TCCATCATCTCTTACAAATCATGTCCCCAGTGGAACAGTCTCTCGACATGCCACATACGGATGGTCATCT CTGCCGTTTCTTGCGCAGCTGTCATCCCCATGATTGTCTGTGCTTCACTAATTTCCATAACCAAGCTGGA GTGGAATCCAAGAGAAAAGGATTATGTATATCACGTAGTGAGTGCGATCTGTGAATGGACAGTGGCCTTT GGTTTTATTTTCTACTTCCTAACTTTCATCCAAGATTTCCAGAGTGTCACCCTAAGGATATCCACAGAAA TCAATGGTGATATT ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG204574 representing NM_018370
Red=Cloning site Green=Tags(s) MLCFLRGMAFVPFLLVTWSSAAFIISYVVAVLSGHVNPFLPYISDTGTTPPESGIFGFMINFSAFLGAAT MYTRYKIVQKQNQTCYFSTPVFNLVSLVLGLVGCFGMGIVANFQELAVPVVHDGGALLAFVCGVVYTLLQ SIISYKSCPQWNSLSTCHIRMVISAVSCAAVIPMIVCASLISITKLEWNPREKDYVYHVVSAICEWTVAF GFIFYFLTFIQDFQSVTLRISTEINGDI TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_018370 |
| ORF Size | 714 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_018370.3 |
| RefSeq Size | 3553 bp |
| RefSeq ORF | 717 bp |
| Locus ID | 55332 |
| UniProt ID | Q8N682 |
| Protein Families | Transmembrane |
| Gene Summary | This gene is regulated as part of the p53 tumor suppressor pathway. The gene encodes a lysosomal membrane protein that is required for the induction of autophagy by the pathway. Decreased transcriptional expression of this gene is associated with various tumors. This gene has a pseudogene on chromosome 4. [provided by RefSeq, Jul 2008] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC204574 | DRAM1 (Myc-DDK-tagged)-Human DNA-damage regulated autophagy modulator 1 (DRAM1) |
CNY 2,400.00 |
|
| RC204574L3 | Lenti ORF clone of Human DNA-damage regulated autophagy modulator 1 (DRAM1), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC204574L4 | Lenti ORF clone of Human DNA-damage regulated autophagy modulator 1 (DRAM1), mGFP tagged |
CNY 5,890.00 |
|
| SC113578 | DRAM1 (untagged)-Human DNA-damage regulated autophagy modulator 1 (DRAM1) |
CNY 1,200.00 |
|
| SC321673 | DRAM1 (untagged)-Human DNA-damage regulated autophagy modulator 1 (DRAM1) |
CNY 2,400.00 |
