DHX30 (NM_138615) Human Tagged ORF Clone
CAT#: RG204467
- TrueORF®
DHX30 (tGFP-tagged) - Human DEAH (Asp-Glu-Ala-His) box polypeptide 30 (DHX30), transcript variant 1
ORF Plasmid: DDK
"NM_138615" in other vectors (6)
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CNY 9,912.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | TurboGFP |
Synonyms | DDX30; NEDMIAL; RETCOR |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RG204467 representing NM_138615
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTTCAGCCTGGACTCATTCAGAAAAGATCGGGCCCAGCACAGGCAGCGTCAGTGCAAACTTCCCCCAC CCCGCCTTCCACCCATGTGTGTCAACCCTACCCCAGGAGGGACCATCTCTCGAGCTTCTAGGGACCTATT AAAAGAGTTCCCACAGCCCAAAAATCTTCTCAACAGTGTGATTGGAAGAGCCCTCGGCATCTCACATGCA AAAGACAAACTAGTCTACGTGCACACAAATGGACCGAAGAAAAAGAAAGTCACACTGCACATAAAATGGC CCAAGAGCGTGGAGGTAGAAGGCTATGGCAGCAAGAAGATCGATGCTGAGCGGCAGGCTGCAGCTGCAGC CTGCCAGCTGTTCAAGGGTTGGGGTCTGCTAGGTCCCCGGAATGAGTTGTTTGACGCAGCCAAATACCGA GTGCTAGCTGATCGCTTTGGCTCCCCTGCCGACAGCTGGTGGCGTCCGGAACCCACCATGCCCCCTACTT CCTGGCGGCAGCTGAATCCAGAGAGTATTCGACCAGGGGGACCTGGGGGCCTATCCCGCTCTTTAGGCCG GGAAGAAGAGGAGGACGAGGAGGAAGAGCTAGAAGAAGGGACCATAGATGTTACCGACTTCTTGTCCATG ACCCAGCAGGATTCCCACGCTCCACTCAGGGACTCAAGGGGGAGTTCCTTTGAGATGACAGATGACGACA GTGCCATTAGGGCTCTGACCCAGTTTCCACTTCCCAAGAACCTTCTGGCCAAGGTGATTCAGATTGCAAC GTCATCCTCCACAGCTAAGAACCTCATGCAGTTCCATACTGTGGGCACCAAGACCAAGCTGTCTACACTC ACCCTGCTCTGGCCCTGCCCCATGACCTTTGTTGCCAAAGGGCGCCGCAAAGCAGAGGCTGAGAATAAGG CGGCAGCCTTGGCCTGCAAGAAACTGAAGAGCCTGGGCCTGGTGGACAGGAACAACGAACCGCTTACACA CGCCATGTATAACCTGGCCTCTTTGCGTGAGCTGGGTGAGACCCAGCGCCGACCATGCACCATCCAGGTG CCCGAGCCCATCCTCCGCAAGATAGAGACCTTCCTGAACCATTACCCTGTGGAGAGTTCATGGATCGCCC CAGAACTCCGGCTGCAGAGTGATGACATCTTGCCCTTGGGCAAGGACTCAGGGCCTCTGAGTGACCCTAT CACAGGCAAGCCCTATGTGCCCCTGTTGGAAGCAGAGGAGGTACGTCTCAGCCAGAGTCTGCTAGAACTG TGGCGGCGGCGAGGGCCGGTCTGGCAGGAGGCCCCCCAGCTACCTGTGGACCCACATCGGGACACCATCC TCAACGCCATTGAGCAGCACCCGGTGGTGGTCATCTCTGGGGACACGGGCTGTGGGAAGACCACGCGCAT CCCCCAGCTGTTGCTGGAGCGCTATGTGACCGAGGGCCGAGGTGCCCGCTGCAATGTTATCATCACCCAA CCTCGCCGCATCTCTGCTGTGTCTGTGGCACAGCGGGTCAGCCACGAACTGGGCCCCTCCCTGCGCCGGA ATGTGGGCTTCCAGGTGCGGTTGGAAAGTAAGCCCCCATCCCGAGGCGGGGCCCTGCTCTTCTGCACTGT GGGTATCCTGCTGCGTAAGCTGCAGAGCAACCCCAGCCTGGAGGGCGTGAGCCACGTCATCGTGGATGAG GTGCATGAGCGGGACGTGAACACAGACTTTCTGCTGATCCTGCTCAAGGGCCTGCAGCGGCTCAACCCGG CCCTGCGGCTGGTGCTCATGAGTGCCACAGGGGACAATGAGCGCTTCTCCCGATACTTTGGTGGCTGCCC CGTCATCAAGGTGCCTGGCTTCATGTACCCAGTCAAGGAGCACTACCTAGAGGACATCCTGGCCAAGTTG GGCAAGCACCAGTACCTGCACCGGCACCGGCACCATGAGTCTGAGGATGAATGCGCACTCGATTTGGACC TTGTGACTGATCTGGTTCTGCACATCGATGCTCGCGGGGAACCAGGTGGGATCCTGTGCTTCCTGCCTGG GTGGCAGGAGATCAAAGGAGTGCAGCAGCGCCTCCAGGAGGCCCTGGGCATGCACGAGAGCAAGTACCTC ATCCTGCCAGTGCACTCCAACATCCCCATGATGGATCAGAAGGCCATATTCCAGCAGCCTCCAGTTGGGG TGCGCAAGATTGTCTTGGCCACCAACATTGCTGAGACTTCCATCACAATCAATGACATCGTGCATGTGGT GGACAGTGGGCTGCACAAGGAAGAACGCTATGACCTGAAGACCAAGGTGTCCTGCCTGGAGACAGTGTGG GTATCAAGAGCCAATGTGATCCAGCGCCGGGGCCGGGCGGGCCGCTGCCAGTCCGGCTTTGCCTACCACT TGTTCCCTCGAAGCCGGCTGGAGAAAATGGTCCCTTTCCAAGTGCCAGAGATCCTGCGCACACCTCTTGA GAACCTGGTGCTGCAAGCGAAAATCCACATGCCTGAGAAGACGGCGGTGGAGTTCCTGTCCAAGGCTGTG GACAGTCCAAACATCAAGGCAGTGGACGAGGCTGTGATCTTGCTCCAGGAGATCGGGGTGCTGGACCAGC GGGAGTACCTGACTACCCTGGGGCAGCGCCTGGCTCACATCTCCACCGACCCCCGGTTGGCCAAGGCCAT TGTGTTGGCTGCCATCTTCCGTTGCCTGCACCCACTACTGGTGGTCGTTTCCTGCCTCACCCGGGACCCC TTCAGCAGCAGCCTACAGAACCGGGCAGAGGTGGACAAGGTGAAAGCACTGTTGAGCCATGACAGCGGCA GTGACCACCTGGCCTTTGTGCGGGCTGTCGCCGGCTGGGAGGAGGTGCTGCGTTGGCAGGACCGCAGCTC CCGGGAGAATTACCTGGAGGAAAACCTGCTGTACGCACCCAGCCTGCGCTTCATCCACGGACTCATCAAG CAGTTCTCAGAGAACATTTATGAGGCCTTCCTGGTGGGGAAGCCCTCGGACTGCACCCTGGCCTCCGCCC AGTGCAACGAGTACAGTGAGGAGGAGGAGCTGGTGAAGGGCGTGCTGATGGCCGGCCTCTACCCCAACCT CATCCAGGTGAGGCAGGGCAAGGTCACCCGGCAGGGGAAGTTCAAGCCCAACAGCGTCACATATAGGACC AAATCAGGCAACATCCTGCTGCACAAGTCGACCATTAACAGGGAGGCCACACGGTTACGGAGCCGATGGC TGACGTATTTCATGGCAGTCAAGTCCAATGGCAGCGTCTTCGTCCGGGACTCCTCTCAGGTGCACCCGCT AGCTGTGCTGCTGCTGACCGACGGGGACGTGCACATCCGTGATGACGGGCGCCGGGCCACCATCTCACTG AGCGACAGTGACCTGCTGCGGCTGGAGGGTGACTCGCGTACCGTGCGGCTGCTGAAGGAGCTGCGGCGGG CCCTGGGCCGCATGGTGGAGCGGAGCCTGCGCAGCGAGCTGGCTGCACTTCCCCCCAGCGTACAGGAGGA GCACGGGCAGCTGCTTGCGCTACTGGCAGAGCTGCTGCGAGGACCCTGTGGCAGCTTTGATGTGCGCAAG ACAGCTGACGAC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG204467 representing NM_138615
Red=Cloning site Green=Tags(s) MFSLDSFRKDRAQHRQRQCKLPPPRLPPMCVNPTPGGTISRASRDLLKEFPQPKNLLNSVIGRALGISHA KDKLVYVHTNGPKKKKVTLHIKWPKSVEVEGYGSKKIDAERQAAAAACQLFKGWGLLGPRNELFDAAKYR VLADRFGSPADSWWRPEPTMPPTSWRQLNPESIRPGGPGGLSRSLGREEEEDEEEELEEGTIDVTDFLSM TQQDSHAPLRDSRGSSFEMTDDDSAIRALTQFPLPKNLLAKVIQIATSSSTAKNLMQFHTVGTKTKLSTL TLLWPCPMTFVAKGRRKAEAENKAAALACKKLKSLGLVDRNNEPLTHAMYNLASLRELGETQRRPCTIQV PEPILRKIETFLNHYPVESSWIAPELRLQSDDILPLGKDSGPLSDPITGKPYVPLLEAEEVRLSQSLLEL WRRRGPVWQEAPQLPVDPHRDTILNAIEQHPVVVISGDTGCGKTTRIPQLLLERYVTEGRGARCNVIITQ PRRISAVSVAQRVSHELGPSLRRNVGFQVRLESKPPSRGGALLFCTVGILLRKLQSNPSLEGVSHVIVDE VHERDVNTDFLLILLKGLQRLNPALRLVLMSATGDNERFSRYFGGCPVIKVPGFMYPVKEHYLEDILAKL GKHQYLHRHRHHESEDECALDLDLVTDLVLHIDARGEPGGILCFLPGWQEIKGVQQRLQEALGMHESKYL ILPVHSNIPMMDQKAIFQQPPVGVRKIVLATNIAETSITINDIVHVVDSGLHKEERYDLKTKVSCLETVW VSRANVIQRRGRAGRCQSGFAYHLFPRSRLEKMVPFQVPEILRTPLENLVLQAKIHMPEKTAVEFLSKAV DSPNIKAVDEAVILLQEIGVLDQREYLTTLGQRLAHISTDPRLAKAIVLAAIFRCLHPLLVVVSCLTRDP FSSSLQNRAEVDKVKALLSHDSGSDHLAFVRAVAGWEEVLRWQDRSSRENYLEENLLYAPSLRFIHGLIK QFSENIYEAFLVGKPSDCTLASAQCNEYSEEEELVKGVLMAGLYPNLIQVRQGKVTRQGKFKPNSVTYRT KSGNILLHKSTINREATRLRSRWLTYFMAVKSNGSVFVRDSSQVHPLAVLLLTDGDVHIRDDGRRATISL SDSDLLRLEGDSRTVRLLKELRRALGRMVERSLRSELAALPPSVQEEHGQLLALLAELLRGPCGSFDVRK TADD TRTRPLE - GFP Tag - V |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_138615 |
ORF Size | 3582 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_138615.3 |
RefSeq Size | 3851 bp |
RefSeq ORF | 3585 bp |
Locus ID | 22907 |
UniProt ID | Q7L2E3 |
Domains | DSRM, DEAD, helicase_C, HA2 |
Gene Summary | DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this DEAD box protein family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. The family member encoded by this gene is a mitochondrial nucleoid protein that associates with mitochondrial DNA. It has also been identified as a component of a transcriptional repressor complex that functions in retinal development, and it is required to optimize the function of the zinc-finger antiviral protein. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Feb 2013] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC204467 | DHX30 (Myc-DDK-tagged)-Human DEAH (Asp-Glu-Ala-His) box polypeptide 30 (DHX30), transcript variant 1 |
CNY 8,312.00 |
|
RC204467L1 | Lenti ORF clone of Human DEAH (Asp-Glu-Ala-His) box polypeptide 30 (DHX30), transcript variant 1, Myc-DDK-tagged |
CNY 10,712.00 |
|
RC204467L2 | Lenti ORF clone of Human DEAH (Asp-Glu-Ala-His) box polypeptide 30 (DHX30), transcript variant 1, mGFP tagged |
CNY 12,160.00 |
|
RC204467L3 | Lenti ORF clone of Human DEAH (Asp-Glu-Ala-His) box polypeptide 30 (DHX30), transcript variant 1, Myc-DDK-tagged |
CNY 12,160.00 |
|
RC204467L4 | Lenti ORF clone of Human DEAH (Asp-Glu-Ala-His) box polypeptide 30 (DHX30), transcript variant 1, mGFP tagged |
CNY 12,160.00 |
|
SC128180 | DHX30 (untagged)-Human DEAH (Asp-Glu-Ala-His) box polypeptide 30 (DHX30), transcript variant 1 |
CNY 10,450.00 |