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CARKL (SHPK) (NM_013276) Human Tagged ORF Clone
CAT＃: RG204421
- TrueORF®
SHPK (tGFP-tagged) - Human sedoheptulokinase (SHPK)
ORF Plasmid: DDK
"NM_013276" in other vectors (5)
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CNY 5,256.00
Product images
CNY 1,999.00
CNY 2,700.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | CARKL; SHK |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG204421 representing NM_013276
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCTGCGCGGCCGATCACCCTCGGCATTGACCTGGGCACCACATCTGTGAAGGCAGCTCTGCTGAGGG CCGCGCCCGACGACCCATCCGGGTTCGCAGTGCTGGCGAGCTGTGCCCGTGCTGCGCGGGCAGAGGCGGC GGTCGAGAGCGCGGTGGCCGGGCCCCAGGGGCGGGAGCAGGATGTGAGTAGAATCCTCCAAGCCCTACAC GAGTGCCTTGCTGCCCTTCCCCGACCCCAGCTCCGGAGCGTCGTGGGCATCGGGGTGTCGGGCCAGATGC ATGGAGTCGTGTTTTGGAAAACAGGCCAAGGCTGTGAATGGACAGAGGGAGGGATTACCCCGGTGTTCGA GCCCCGAGCTGTTAGCCACCTGGTCACGTGGCAGGATGGCCGATGTAGCAGCGAATTCCTGGCCTCTCTG CCCCAGCCGAAGTCTCATCTCAGTGTGGCCACGGGCTTCGGCTGTGCAACCATCTTCTGGCTTTTGAAAT ATCGCCCAGAGTTCCTGAAGTCCTACGACGCAGCCGGTACCATCCACGACTATGTGGTTGCCATGCTGTG TGGCTTGCCAAGACCTCTGATGTCCGACCAGAATGCTGCCAGCTGGGGCTATTTCAACACGCAGAGCCAA AGCTGGAACGTAGAGACACTGAGGAGCTCGGGTTTTCCTGTCCACCTGCTCCCAGACATCGCCGAGCCTG GCAGTGTGGCGGGCAGAACTTCCCACATGTGGTTTGAAATCCCAAAGGGGACGCAGGTGGGAGTGGCCTT GGGTGATTTACAGGCCTCTGTCTATTCCTGCATGGCCCAGAGGACAGATGCAGTTCTCAACATCAGCACC TCGGTTCAGCTGGCAGCCTCCATGCCTTCAGGATTCCAGCCTGCACAGACTCCAGACCCTACGGCCCCAG TCGCCTACTTCCCATACTTCAACAGGACCTACCTGGGGGTGGCCGCGTCACTCAACGGGGGCAATGTGCT GGCCACGTTCGTCCACATGCTGGTTCAGTGGATGGCAGATCTAGGCCTGGAGGTTGAAGAATCCACTGTG TATTCACGCATGATTCAGGCAGCTGTGCAGCAGAGAGATACCCACCTGACCATCACCCCGACAGTGCTGG GGGAGAGGCACCTGCCGGACCAGCTGGCCTCAGTGACCAGAATCTCCTCCTCCGACCTCTCCCTGGGGCA CGTGACCCGGGCTCTGTGCCGAGGCATTGTTCAGAACCTGCACTCCATGCTTCCGATTCAGCAGCTCCAG GAGTGGGGCGTGGAGAGGGTGATGGGCAGTGGGAGTGCGCTGTCCAGGAATGACGTGCTGAAGCAGGAGG TGCAGAGGGCTTTCCCTTTGCCCATGTCCTTTGGGCAGGATGTGGATGCAGCTGTCGGGGCAGCTCTGGT CATGCTCCGGAGACACCTCAACCAGAAGGAATCT ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG204421 representing NM_013276
Red=Cloning site Green=Tags(s) MAARPITLGIDLGTTSVKAALLRAAPDDPSGFAVLASCARAARAEAAVESAVAGPQGREQDVSRILQALH ECLAALPRPQLRSVVGIGVSGQMHGVVFWKTGQGCEWTEGGITPVFEPRAVSHLVTWQDGRCSSEFLASL PQPKSHLSVATGFGCATIFWLLKYRPEFLKSYDAAGTIHDYVVAMLCGLPRPLMSDQNAASWGYFNTQSQ SWNVETLRSSGFPVHLLPDIAEPGSVAGRTSHMWFEIPKGTQVGVALGDLQASVYSCMAQRTDAVLNIST SVQLAASMPSGFQPAQTPDPTAPVAYFPYFNRTYLGVAASLNGGNVLATFVHMLVQWMADLGLEVEESTV YSRMIQAAVQQRDTHLTITPTVLGERHLPDQLASVTRISSSDLSLGHVTRALCRGIVQNLHSMLPIQQLQ EWGVERVMGSGSALSRNDVLKQEVQRAFPLPMSFGQDVDAAVGAALVMLRRHLNQKES TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_013276 |
| ORF Size | 1434 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_013276.4 |
| RefSeq Size | 3838 bp |
| RefSeq ORF | 1437 bp |
| Locus ID | 23729 |
| UniProt ID | Q9UHJ6 |
| Domains | FGGY |
| Protein Families | Druggable Genome |
| Gene Summary | The protein encoded by this gene has weak homology to several carbohydrate kinases, a class of proteins involved in the phosphorylation of sugars as they enter a cell, inhibiting return across the cell membrane. Sequence variation between this novel gene and known carbohydrate kinases suggests the possibility of a different substrate, cofactor or changes in kinetic properties distinguishing it from other carbohydrate kinases. The gene resides in a region commonly deleted in cystinosis patients, suggesting a role as a modifier for the cystinosis phenotype. The genomic region is also rich in Alu repetitive sequences, frequently involved in chromosomal rearrangements. [provided by RefSeq, Jul 2008] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC204421 | SHPK (Myc-DDK-tagged)-Human sedoheptulokinase (SHPK) |
CNY 3,656.00 |
|
| RC204421L3 | Lenti ORF clone of Human sedoheptulokinase (SHPK), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC204421L4 | Lenti ORF clone of Human sedoheptulokinase (SHPK), mGFP tagged |
CNY 5,890.00 |
|
| SC111213 | SHPK (untagged)-Human sedoheptulokinase (SHPK) |
CNY 3,656.00 |
|
| SC324665 | SHPK (untagged)-Human sedoheptulokinase (SHPK) |
CNY 3,656.00 |
