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RHBDF1 (NM_022450) Human Tagged ORF Clone
CAT＃: RG204201
- TrueORF®
RHBDF1 (tGFP-tagged) - Human rhomboid 5 homolog 1 (Drosophila) (RHBDF1)
ORF Plasmid: DDK
"NM_022450" in other vectors (5)
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CNY 4,370.00
Product images
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | C16orf8; Dist1; EGFR-RS; gene-89; gene-90; hDist1 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG204201 representing NM_022450
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGTGAGGCCCGCAGGGACAGCACGAGCAGCCTGCAGCGCAAGAAGCCACCCTGGCTAAAGCTGGACA TTCCCTCTGCGGTGCCCCTGACGGCAGAAGAGCCCAGCTTCCTGCAGCCCCTGAGGCGACAGGCTTTCCT GAGGAGTGTGAGTATGCCAGCCGAGACAGCCCACATCTCTTCACCCCACCATGAGCTCCGGCGGCCGGTG CTGCAACGCCAGACGTCCATCACACAGACCATCCGCAGGGGGACCGCCGACTGGTTTGGAGTGAGCAAGG ACAGTGACAGCACCCAGAAATGGCAGCGCAAGAGCATCCGTCACTGCAGCCAGCGCTACGGGAAGCTGAA GCCCCAGGTCCTCCGGGAGCTGGACCTGCCCAGCCAGGACAACGTGTCGCTGACCAGCACCGAGACGCCA CCCCCACTCTACGTGGGGCCATGCCAGCTGGGCATGCAGAAGATCATAGACCCCCTGGCCCGTGGCCGTG CCTTCCGTGTGGCAGATGACACTGCGGAAGGCCTGAGTGCCCCACACACTCCCGTCACGCCGGGTGCTGC CTCCCTCTGCTCCTTCTCCAGCTCCCGCTCAGGTTTCCACCGGCTCCCGCGGCGGCGCAAGCGAGAGTCG GTGGCCAAGATGAGCTTCCGGGCGGCCGCAGCGCTGATGAAAGGCCGCTCCGTTAGGGATGGCACCTTTC GCCGGGCACGGCGTCGAAGCTTCACTCCAGCTAGCTTTCTGGAGGAGGACACAACTGATTTCCCCGATGA GCTGGACACATCCTTCTTTGCCCGGGAAGGTATCCTCCATGAAGAGCTGTCCACATACCCGGATGAAGTT TTCGAGTCCCCATCGGAGGCAGCGCTAAAGGACTGGGAGAAGGCACCGGAGCAGGCGGACCTCACCGGCG GGGCCCTGGACCGCAGCGAGCTTGAGCGCAGCCACCTGATGCTGCCCTTGGAGCGAGGCTGGCGGAAGCA GAAGGAGGGCGCCGCAGCCCCGCAGCCCAAGGTGCGGCTCCGACAGGAGGTGGTGAGCACCGCGGGGCCG CGACGGGGCCAGCGTATCGCGGTGCCGGTGCGCAAGCTCTTCGCCCGGGAGAAGCGGCCGTATGGGCTGG GCATGGTGGGACGGCTCACCAACCGCACCTACCGCAAGCGCATCGACAGCTTCGTCAAGCGCCAGATCGA GGACATGGACGACCACAGGCCCTTCTTCACCTACTGGCTTACCTTCGTGCACTCGCTCGTCACCATCCTA GCCGTGTGCATCTATGGCATCGCGCCCGTGGGCTTCTCGCAGCATGAGACGGTGGACTCGGTGCTGCGGA ACCGCGGGGTCTACGAGAACGTCAAGTACGTGCAGCAGGAGAACTTCTGGATCGGGCCCAGCTCGGAGGC CCTCATCCACCTGGGCGCCAAGTTTTCGCCCTGCATGCGCCAGGACCCGCAGGTGCACAGCTTCATTCGC TCGGCGCGCGAGCGCGAGAAGCACTCCGCCTGCTGCGTGCGCAACGACAGGTCGGGCTGCGTGCAGACCT CGGAGGAGGAGTGCTCGTCCACGCTGGCAGTGTGGGTGAAGTGGCCCATCCATCCCAGCGCCCCAGAGCT TGCGGGCCACAAGAGACAGTTTGGCTCTGTCTGCCACCAGGATCCCAGGGTGTGTGATGAGCCCTCCTCC GAAGACCCTCATGAGTGGCCAGAAGACATCACCAAGTGGCCGATCTGCACCAAAAACAGCGCTGGGAACC ACACCAACCATCCCCACATGGACTGTGTCATCACAGGACGGCCCTGCTGCATTGGCACCAAGGGCAGGTG TGAGATCACCTCCCGGGAGTACTGTGACTTCATGAGGGGCTACTTCCATGAGGAGGCCACGCTCTGCTCT CAGGTGCACTGCATGGATGATGTGTGTGGGCTCCTGCCTTTTCTCAACCCCGAGGTGCCTGACCAGTTCT ACCGCCTGTGGCTATCCCTCTTCCTGCACGCCGGGATCTTGCACTGCCTGGTGTCCATCTGCTTCCAGAT GACTGTCCTGCGGGACCTGGAGAAGCTGGCAGGCTGGCACCGCATAGCCATCATCTACCTGCTGAGTGGT GTCACCGGCAACCTGGCCAGTGCCATCTTCCTGCCATACCGAGCAGAGGTGGGTCCTGCTGGCTCCCAGT TCGGCATCCTGGCCTGCCTCTTCGTGGAGCTCTTCCAGAGCTGGCAGATCCTGGCGCGGCCCTGGCGTGC CTTCTTCAAGCTGCTGGCTGTGGTGCTCTTCCTCTTCACCTTTGGGCTGCTGCCGTGGATTGACAACTTT GCCCACATCTCGGGGTTCATCAGTGGCCTCTTCCTCTCCTTCGCCTTCTTGCCCTACATCAGCTTTGGCA AGTTCGACCTGTACCGGAAACGCTGCCAGATCATCATCTTTCAGGTGGTCTTCCTGGGCCTCCTGGCTGG CCTGGTGGTCCTCTTCTACGTCTATCCTGTCCGCTGTGAGTGGTGTGAGTTCCTCACCTGCATCCCCTTC ACTGACAAGTTCTGTGAGAAGTACGAACTGGACGCTCAGCTCCAC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG204201 representing NM_022450
Red=Cloning site Green=Tags(s) MSEARRDSTSSLQRKKPPWLKLDIPSAVPLTAEEPSFLQPLRRQAFLRSVSMPAETAHISSPHHELRRPV LQRQTSITQTIRRGTADWFGVSKDSDSTQKWQRKSIRHCSQRYGKLKPQVLRELDLPSQDNVSLTSTETP PPLYVGPCQLGMQKIIDPLARGRAFRVADDTAEGLSAPHTPVTPGAASLCSFSSSRSGFHRLPRRRKRES VAKMSFRAAAALMKGRSVRDGTFRRARRRSFTPASFLEEDTTDFPDELDTSFFAREGILHEELSTYPDEV FESPSEAALKDWEKAPEQADLTGGALDRSELERSHLMLPLERGWRKQKEGAAAPQPKVRLRQEVVSTAGP RRGQRIAVPVRKLFAREKRPYGLGMVGRLTNRTYRKRIDSFVKRQIEDMDDHRPFFTYWLTFVHSLVTIL AVCIYGIAPVGFSQHETVDSVLRNRGVYENVKYVQQENFWIGPSSEALIHLGAKFSPCMRQDPQVHSFIR SAREREKHSACCVRNDRSGCVQTSEEECSSTLAVWVKWPIHPSAPELAGHKRQFGSVCHQDPRVCDEPSS EDPHEWPEDITKWPICTKNSAGNHTNHPHMDCVITGRPCCIGTKGRCEITSREYCDFMRGYFHEEATLCS QVHCMDDVCGLLPFLNPEVPDQFYRLWLSLFLHAGILHCLVSICFQMTVLRDLEKLAGWHRIAIIYLLSG VTGNLASAIFLPYRAEVGPAGSQFGILACLFVELFQSWQILARPWRAFFKLLAVVLFLFTFGLLPWIDNF AHISGFISGLFLSFAFLPYISFGKFDLYRKRCQIIIFQVVFLGLLAGLVVLFYVYPVRCEWCEFLTCIPF TDKFCEKYELDAQLH TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_022450 |
| ORF Size | 2565 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_022450.2, NP_071895.2 |
| RefSeq Size | 3053 bp |
| RefSeq ORF | 2568 bp |
| Locus ID | 64285 |
| UniProt ID | Q96CC6 |
| Domains | Rhomboid |
| Protein Families | Druggable Genome, Protease, Transmembrane |
| Gene Summary | Regulates ADAM17 protease, a sheddase of the epidermal growth factor (EGF) receptor ligands and TNF, thereby plays a role in sleep, cell survival, proliferation, migration and inflammation. Does not exhibit any protease activity on its own.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC204201 | RHBDF1 (Myc-DDK-tagged)-Human rhomboid 5 homolog 1 (Drosophila) (RHBDF1) |
CNY 3,990.00 |
|
| RC204201L3 | Lenti ORF clone of Human rhomboid 5 homolog 1 (Drosophila) (RHBDF1), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC204201L4 | Lenti ORF clone of Human rhomboid 5 homolog 1 (Drosophila) (RHBDF1), mGFP tagged |
CNY 5,890.00 |
|
| SC112515 | RHBDF1 (untagged)-Human rhomboid 5 homolog 1 (Drosophila) (RHBDF1) |
CNY 2,950.00 |
|
| SC319667 | RHBDF1 (untagged)-Human rhomboid 5 homolog 1 (Drosophila) (RHBDF1) |
CNY 6,272.00 |
