Seryl tRNA synthetase (SARS) (NM_006513) Human Tagged ORF Clone
CAT#: RG203665
- TrueORF®
SARS (tGFP-tagged) - Human seryl-tRNA synthetase (SARS), transcript variant 1
ORF Plasmid: DDK
"NM_006513" in other vectors (4)
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CNY 5,432.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | TurboGFP |
Synonyms | NEDMAS; SARS; SERRS; SERS |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RG203665 representing NM_006513
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGTGCTGGATCTGGATTTGTTTCGGGTGGATAAAGGAGGGGACCCAGCCCTCATCCGAGAGACGCAGG AGAAGCGCTTCAAGGACCCGGGACTAGTGGACCAGCTGGTGAAGGCAGACAGCGAGTGGCGACGATGTAG ATTTCGGGCAGACAACTTGAACAAGCTGAAGAACCTATGCAGCAAGACAATCGGAGAGAAAATGAAGAAA AAAGAGCCAGTGGGAGATGATGAGTCTGTCCCAGAGAATGTGCTGAGTTTCGATGACCTTACTGCAGACG CTTTAGCTAACCTGAAAGTCTCACAAATCAAAAAAGTCCGACTCCTCATTGATGAAGCCATCCTGAAGTG TGACGCGGAGCGGATAAAGTTGGAAGCAGAGCGGTTTGAGAACCTCCGAGAGATTGGGAACCTTCTGCAC CCTTCTGTACCCATCAGTAACGATGAGGATGTGGACAACAAAGTAGAGAGGATTTGGGGTGATTGTACAG TCAGGAAGAAGTACTCTCATGTGGACCTGGTGGTGATGGTAGATGGCTTTGAAGGCGAAAAGGGGGCCGT GGTGGCTGGGAGTCGAGGGTACTTCTTGAAGGGGGTCCTGGTGTTCCTGGAACAGGCTCTCATCCAGTAT GCCCTTCGCACCTTGGGAAGTCGGGGCTACATTCCCATTTATACCCCCTTTTTCATGAGGAAGGAGGTCA TGCAGGAGGTGGCACAGCTCAGCCAGTTTGATGAAGAACTTTATAAGGTGATTGGCAAAGGCAGTGAAAA GTCTGATGACAACTCCTATGATGAGAAGTACCTGATTGCCACCTCAGAGCAGCCCATTGCTGCCCTGCAC CGGGATGAGTGGCTCCGGCCGGAGGACCTGCCCATCAAGTATGCTGGCCTGTCTACCTGCTTCCGTCAGG AGGTGGGCTCCCATGGCCGTGACACCCGTGGCATCTTCCGAGTCCATCAGTTTGAGAAGATTGAACAGTT TGTGTACTCATCACCCCATGACAACAAGTCATGGGAGATGTTTGAAGAGATGATTACCACCGCAGAGGAG TTCTACCAGTCCCTGGGGATTCCTTACCACATTGTGAATATTGTCTCAGGTTCTTTGAATCATGCTGCCA GTAAGAAGCTTGACCTGGAGGCCTGGTTTCCGGGCTCAGGAGCCTTCCGTGAGTTGGTCTCCTGTTCTAA TTGCACGGATTACCAGGCTCGCCGGCTTCGAATCCGATATGGGCAAACCAAGAAGATGATGGACAAGGTG GAGTTTGTCCATATGCTCAATGCTACCATGTGCGCCACTACCTGTACCATCTGCGCCATCCTGGAGAACT ACCAGACAGAGAAGGGCATCACTGTGCCTGAGAAATTGAAGGAGTTCATGCCGCCAGGACTGCAAGAACT GATCCCCTTTGTGAAGCCTGCGCCCATTGAGCAGGAGCCATCAAAGAAGCAGAAGAAGCAACATGAGGGC AGCAAAAAGAAAGCAGCAGCAAGAGACGTCACCCTAGAAAACAGGCTGCAGAACATGGAGGTCACCGATG CT ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG203665 representing NM_006513
Red=Cloning site Green=Tags(s) MVLDLDLFRVDKGGDPALIRETQEKRFKDPGLVDQLVKADSEWRRCRFRADNLNKLKNLCSKTIGEKMKK KEPVGDDESVPENVLSFDDLTADALANLKVSQIKKVRLLIDEAILKCDAERIKLEAERFENLREIGNLLH PSVPISNDEDVDNKVERIWGDCTVRKKYSHVDLVVMVDGFEGEKGAVVAGSRGYFLKGVLVFLEQALIQY ALRTLGSRGYIPIYTPFFMRKEVMQEVAQLSQFDEELYKVIGKGSEKSDDNSYDEKYLIATSEQPIAALH RDEWLRPEDLPIKYAGLSTCFRQEVGSHGRDTRGIFRVHQFEKIEQFVYSSPHDNKSWEMFEEMITTAEE FYQSLGIPYHIVNIVSGSLNHAASKKLDLEAWFPGSGAFRELVSCSNCTDYQARRLRIRYGQTKKMMDKV EFVHMLNATMCATTCTICAILENYQTEKGITVPEKLKEFMPPGLQELIPFVKPAPIEQEPSKKQKKQHEG SKKKAAARDVTLENRLQNMEVTDA TRTRPLE - GFP Tag - V |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_006513 |
ORF Size | 1542 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_006513.2, NP_006504.2 |
RefSeq Size | 1942 bp |
RefSeq ORF | 1545 bp |
Locus ID | 6301 |
UniProt ID | P49591 |
Domains | tRNA-synt_2b, Seryl_tRNA_N |
Protein Pathways | Aminoacyl-tRNA biosynthesis |
Gene Summary | This gene belongs to the class II amino-acyl tRNA family. The encoded enzyme catalyzes the transfer of L-serine to tRNA (Ser) and is related to bacterial and yeast counterparts. Multiple alternatively spliced transcript variants have been described but the biological validity of all variants is unknown. [provided by RefSeq, Jul 2010] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC203665 | SARS (Myc-DDK-tagged)-Human seryl-tRNA synthetase (SARS), transcript variant 1 |
CNY 3,832.00 |
|
RC203665L3 | Lenti ORF clone of Human seryl-tRNA synthetase (SARS), transcript variant 1, Myc-DDK-tagged |
CNY 6,460.00 |
|
RC203665L4 | Lenti ORF clone of Human seryl-tRNA synthetase (SARS), transcript variant 1, mGFP tagged |
CNY 6,460.00 |
|
SC319289 | SARS (untagged)-Human seryl-tRNA synthetase (SARS), transcript variant 1 |
CNY 3,832.00 |
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