TBCC (NM_003192) Human Tagged ORF Clone

CAT#: RG203563

  • TrueORF®

TBCC (tGFP-tagged) - Human tubulin folding cofactor C (TBCC)

ORF Plasmid: DDK tGFP



  "NM_003192" in other vectors (4)

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CNY 5,256.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (5)
pCMV6-AC-GFP, mammalian vector with C-terminal tGFP tag, 10ug
    • 10 ug

CNY 6,080.00


Mouse monoclonal turboGFP antibody, clone OTI2H8
    • 100 ul

CNY 1,000.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


TBCC mouse monoclonal antibody, clone OTI4C4 (formerly 4C4)
    • 100 ul

CNY 1,999.00
CNY 2,700.00

Specifications

Product Data
Type Human Tagged ORF Clone
Tag TurboGFP
Synonyms CFC
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RG203563 representing NM_003192
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGAGTCCGTCAGTTGCTCCGCTGCTGCTGTCAGGACCGGAGACATGGAGTCCCAGCGGGACCTGAGCC
TGGTGCCTGAGCGGCTTCAGAGACGCGAACAAGAACGGCAGCTGGAAGTTGAAAGGCGGAAACAAAAGCG
GCAGAACCAGGAGGTAGAGAAGGAGAACAGCCACTTTTTCGTCGCCACCTTTGCTCGGGAGCGAGCGGCC
GTGGAAGAGCTTCTGGAGCGCGCGGAGTCGGTCGAGCGGCTGGAGGAGGCGGCCTCTCGGCTCCAGGGGC
TGCAGAAACTAATCAACGACTCAGTTTTTTTCCTAGCCGCTTACGACCTGCGGCAGGGACAAGAGGCGCT
GGCGCGGCTGCAGGCGGCCTTGGCCGAGCGGCGCCGGGGGCTGCAGCCCAAGAAGCGTTTCGCTTTCAAG
ACCCGGGGAAAGGATGCTGCTTCGTCTACCAAAGTAGACGCGGCTCCTGGCATCCCCCCGGCAGTTGAAA
GCATACAGGACTCCCCGCTGCCCAAGAAGGCGGAAGGAGACCTCGGCCCCAGCTGGGTCTGCGGTTTCTC
CAACCTGGAGTCCCAAGTCTTGGAGAAGAGAGCCAGCGAGTTGCACCAGCGCGACGTTCTTTTGACCGAA
CTGAGCAACTGCACGGTCAGACTTTATGGAAATCCCAACACCCTGCGGCTAACCAAGGCCCACAGCTGCA
AGCTGCTCTGCGGTCCGGTGTCTACCTCTGTTTTCCTGGAGGACTGCAGTGACTGCGTGCTGGCAGTGGC
CTGCCAACAGCTCCGCATACACAGTACGAAAGACACCCGCATCTTCCTGCAGGTGACCAGCAGGGCCATC
GTGGAGGACTGCAGTGGGATCCAGTTCGCCCCTTACACCTGGAGCTACCCGGAGATCGACAAGGACTTCG
AGAGCTCTGGTTTAGATAGGAGCAAAAATAACTGGAACGATGTTGACGATTTTAACTGGCTGGCCCGGGA
TATGGCCTCCCCAAACTGGAGTATTCTTCCTGAAGAGGAGCGAAATATCCAGTGGGAC


ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
>RG203563 representing NM_003192
Red=Cloning site Green=Tags(s)

MESVSCSAAAVRTGDMESQRDLSLVPERLQRREQERQLEVERRKQKRQNQEVEKENSHFFVATFARERAA
VEELLERAESVERLEEAASRLQGLQKLINDSVFFLAAYDLRQGQEALARLQAALAERRRGLQPKKRFAFK
TRGKDAASSTKVDAAPGIPPAVESIQDSPLPKKAEGDLGPSWVCGFSNLESQVLEKRASELHQRDVLLTE
LSNCTVRLYGNPNTLRLTKAHSCKLLCGPVSTSVFLEDCSDCVLAVACQQLRIHSTKDTRIFLQVTSRAI
VEDCSGIQFAPYTWSYPEIDKDFESSGLDRSKNNWNDVDDFNWLARDMASPNWSILPEEERNIQWD

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_003192
ORF Size 1038 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_003192.2, NP_003183.1
RefSeq Size 1675 bp
RefSeq ORF 1041 bp
Locus ID 6903
UniProt ID Q15814
Domains CARP
Gene Summary Cofactor C is one of four proteins (cofactors A, D, E, and C) involved in the pathway leading to correctly folded beta-tubulin from folding intermediates. Cofactors A and D are believed to play a role in capturing and stabilizing beta-tubulin intermediates in a quasi-native confirmation. Cofactor E binds to the cofactor D/beta-tubulin complex; interaction with cofactor C then causes the release of beta-tubulin polypeptides that are committed to the native state. [provided by RefSeq, Jul 2008]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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