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KRTHA3B (KRT33B) (NM_002279) Human Tagged ORF Clone
CAT＃: RG203503
- TrueORF®
KRT33B (tGFP-tagged) - Human keratin 33B (KRT33B)
ORF Plasmid: DDK
"NM_002279" in other vectors (4)
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CNY 5,256.00
Product images
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | Ha-3II; HA3II; hHa3-II; K33B; KRTHA3A; KRTHA3B |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG203503 representing NM_002279
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCCTACAACTTCTGCCTGCCCAGCCTGAGCTGCCGCACCAGCTGCTCCTCCCGGCCCTGTGTGCCCC CCAGCTGCCACGGCTACACCCTGCCCGGGGCCTGCAACATCCCTGCCAATGTGAGCAACTGCAACTGGTT CTGCGAGGGCTCCTTCAATGGCAGCGAGAAGGAGACTATGCAGTTCCTGAACGACCGCCTGGCCAGCTAC CTGGAGAAGGTGCGTCAGCTGGAGCGGGACAACGCGGAGCTGGAGAACCTCATCCGGGAGCGGTCTCAGC AGCAGGAGCCCTTGCTGTGCCCCAGCTACCAGTCCTACTTCAAGACCATTGAGGAGCTCCAGCAGAAGAT CCTGTGCAGCAAGTCTGAGAATGCCAGGCTGGTGGTGCAGATCGACAATGCCAAGCTGGCTGCAGATGAC TTCAGAACCAAGTACCAGACGGAGCAGTCCCTGCGGCAGCTGGTGGAGTCCGACATCAACAGCCTGCGCA GGATTCTGGATGAGCTGACCCTGTGCAGGTCTGACCTGGAGGCCCAGATGGAGTCCCTGAAGGAGGAGCT GCTGTCCCTCAAGCAGAACCATGAGCAGGAAGTCAACACCTTGCGCTGCCAGCTTGGAGACCGCCTCAAC GTGGAGGTGGACGCTGCTCCCGCTGTGGACCTGAACCAGGTCCTGAACGAGACCAGGAATCAGTATGAGG CCCTGGTGGAAACCAACCGCAGGGAAGTGGAGCAATGGTTCGCCACGCAGACCGAGGAGCTGAACAAGCA GGTGGTATCCAGCTCGGAGCAGCTGCAGTCCTACCAGGCGGAGATCATCGAGCTGAGACGCACAGTCAAT GCCCTGGAGATCGAGCTGCAGGCCCAGCACAACCTGCGATACTCTCTGGAAAACACGCTGACAGAGAGCG AGGCCCGCTACAGCTCCCAGCTGTCCCAGGTGCAGAGCCTGATCACCAACGTGGAGTCCCAGCTGGCGGA GATCCGCAGTGACCTGGAGCGGCAGAACCAGGAGTATCAGGTGCTGCTGGACGTGCGGGCGCGGCTGGAG TGTGAGATCAACACATACCGGAGCCTGCTGGAGAGCGAGGACTGCAAGCTGCCCTCCAACCCCTGCGCCA CCACCAATGCATGTGAAAAGCCCATTGGATCCTGTGTCACCAATCCTTGTGGTCCTCGTTCCCGCTGTGG GCCTTGCAACACCTTTGGGTAC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG203503 representing NM_002279
Red=Cloning site Green=Tags(s) MPYNFCLPSLSCRTSCSSRPCVPPSCHGYTLPGACNIPANVSNCNWFCEGSFNGSEKETMQFLNDRLASY LEKVRQLERDNAELENLIRERSQQQEPLLCPSYQSYFKTIEELQQKILCSKSENARLVVQIDNAKLAADD FRTKYQTEQSLRQLVESDINSLRRILDELTLCRSDLEAQMESLKEELLSLKQNHEQEVNTLRCQLGDRLN VEVDAAPAVDLNQVLNETRNQYEALVETNRREVEQWFATQTEELNKQVVSSSEQLQSYQAEIIELRRTVN ALEIELQAQHNLRYSLENTLTESEARYSSQLSQVQSLITNVESQLAEIRSDLERQNQEYQVLLDVRARLE CEINTYRSLLESEDCKLPSNPCATTNACEKPIGSCVTNPCGPRSRCGPCNTFGY TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_002279 |
| ORF Size | 1212 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_002279.5 |
| RefSeq Size | 1621 bp |
| RefSeq ORF | 1215 bp |
| Locus ID | 3884 |
| UniProt ID | Q14525 |
| Gene Summary | This gene encodes a member of the keratin gene family. This gene is one of multiple type I hair keratin genes that are clustered in a region of chromosome 17q12-q21 and have the same direction of transcription. As a type I hair keratin, the encoded protein is an acidic protein which heterodimerizes with type II keratins to form hair and nails. There are two isoforms of this protein, encoded by two separate genes, keratin 33A and keratin 33B. [provided by RefSeq, May 2012] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC203503 | KRT33B (Myc-DDK-tagged)-Human keratin 33B (KRT33B) |
CNY 3,656.00 |
|
| RC203503L3 | Lenti ORF clone of Human keratin 33B (KRT33B), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC203503L4 | Lenti ORF clone of Human keratin 33B (KRT33B), mGFP tagged |
CNY 5,890.00 |
|
| SC122624 | KRT33B (untagged)-Human keratin 33B (KRT33B) |
CNY 3,656.00 |
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