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DUS3L (NM_020175) Human Tagged ORF Clone
CAT＃: RG203380
- TrueORF®
DUS3L (tGFP-tagged) - Human dihydrouridine synthase 3-like (S. cerevisiae) (DUS3L), transcript variant 1
ORF Plasmid: DDK
"NM_020175" in other vectors (5)
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CNY 5,040.00
Product images
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | DUS3 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG203380 representing NM_020175
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGGAGGGAACGGCGGAGGCTCCTCTAGAGAATGGTGGTGGTGGCGACTCGGGAGCCGGAGCTTTGG AACGAGGAGTGGCGCCCATTAAGCGTCAATACCTCACCACCAAGGAGCAGTTTCACCAATTCCTGGAAGC CAAAGGGCAGGAGAAGACTTGCCGGGAAACCGAGGTAGGAGACCCTGCTGGCAATGAGCTGGCTGAGCCT GAGGCTAAGCGGATCCGACTGGAGGATGGACAGACGGCGGACGGGCAGACGGAGGAGGCAGCAGAGCCCG GGGAGCAGCTACAGACTCAGAAGAGGGCCCGGGGACAAAACAAGGGCCGGCCCCATGTGAAGCCCACGAA CTACGACAAGAACAGGCTGTGTCCCTCCCTAATCCAGGAGTCGGCTGCTAAGTGTTTCTTCGGTGATCGC TGCCGCTTTCTGCACGACGTGGGGCGCTACCTGGAGACCAAGCCGGCCGACCTGGGCCCCCGCTGCGTGC TCTTCGAGACCTTCGGCCGGTGCCCCTACGGCGTGACCTGCCGCTTCGCTGGGGCCCACCTGGGGCCCGA GGGACAGAACCTGGTGCAGGAGGAGTTGGCGGCCCGCGGGACCCAGCCCCCGTCCATCCGCAACGGCCTG GACAAAGCCCTGCAGCAGCAGCTGCGGAAGCGCGAGGTCCGCTTCGAGCGAGCTGAGCAGGCCCTGCGCC GGTTCAGCCAGGGCCCCACACCCGCTGCCGCTGTCCCCGAGGGCACGGCAGCCGAGGGCGCTCCCAGGCA GGAAAACTGTGGTGCCCAGCAGGTCCCCGCAGGGCCGGGCACTAGCACCCCTCCCAGCAGCCCCGTGCGG ACCTGCGGGCCCCTGACGGATGAGGACGTGGTCAGGCTGCGGCCCTGTGAGAAGAAGCGGCTGGACATCC GTGGCAAACTTTACCTGGCCCCCCTCACCACGTGTGGGAACCTGCCCTTCCGACGGATCTGCAAGCGCTT CGGGGCGGATGTGACATGTGGAGAGATGGCCGTCTGCACCAACCTGCTGCAGGGCCAGATGTCCGAGTGG GCCCTACTCAAACGCCACCAGTGTGAGGACATCTTTGGCGTCCAGCTGGAGGGCGCCTTCCCCGACACCA TGACCAAGTGTGCCGAGCTGCTGAGCCGCACCGTGGAGGTGGACTTTGTGGACATCAACGTCGGCTGCCC CATCGACCTCGTGTACAAGAAGGGTGGGGGCTGTGCCCTCATGAATCGCTCCACCAAGTTCCAGCAGATC GTCCGTGGCATGAACCAGGTGCTGGATGTGCCGCTGACTGTGAAGATCCGCACAGGCGTCCAGGAGCGTG TGAACCTGGCGCACCGCCTGCTGCCCGAGCTGCGGGACTGGGGCGTGGCACTCGTCACGCTCCACGGCCG CTCTCGGGAGCAGCGCTACACCAAGCTAGCTGACTGGCAGTACATCGAGGAGTGCGTGCAGGCCGCCAGC CCCATGCCCCTGTTCGGAAATGGGGACATCTTGTCATTTGAGGATGCCAACCGCGCCATGCAGACTGGTG TCACCGGGATCATGATTGCCCGTGGCGCCCTGCTCAAGCCGTGGCTCTTCACGGAGATCAAGGAGCAGCG GCACTGGGACATCTCGTCGTCCGAGCGCCTGGACATCCTGCGGGACTTCACCAACTACGGCCTGGAGCAC TGGGGCTCGGACACGCAGGGCGTGGAGAAGACCCGGCGCTTTCTGCTCGAGTGGCTGTCCTTCCTGTGCC GGTACGTGCCCGTGGGGCTGCTGGAGCGGCTCCCACAGAGGATCAACGAGCGGCCGCCCTACTACCTGGG CCGCGACTACCTGGAGACGCTGATGGCCAGCCAGAAGGCAGCCGACTGGATCCGCATCAGCGAGATGCTC CTTGGGCCAGTGCCCCCCAGCTTCGCCTTCTTGCCGAAGCACAAGGCCAACGCGTACAAG AGCGGACCGACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG203380 representing NM_020175
Red=Cloning site Green=Tags(s) MAEGTAEAPLENGGGGDSGAGALERGVAPIKRQYLTTKEQFHQFLEAKGQEKTCRETEVGDPAGNELAEP EAKRIRLEDGQTADGQTEEAAEPGEQLQTQKRARGQNKGRPHVKPTNYDKNRLCPSLIQESAAKCFFGDR CRFLHDVGRYLETKPADLGPRCVLFETFGRCPYGVTCRFAGAHLGPEGQNLVQEELAARGTQPPSIRNGL DKALQQQLRKREVRFERAEQALRRFSQGPTPAAAVPEGTAAEGAPRQENCGAQQVPAGPGTSTPPSSPVR TCGPLTDEDVVRLRPCEKKRLDIRGKLYLAPLTTCGNLPFRRICKRFGADVTCGEMAVCTNLLQGQMSEW ALLKRHQCEDIFGVQLEGAFPDTMTKCAELLSRTVEVDFVDINVGCPIDLVYKKGGGCALMNRSTKFQQI VRGMNQVLDVPLTVKIRTGVQERVNLAHRLLPELRDWGVALVTLHGRSREQRYTKLADWQYIEECVQAAS PMPLFGNGDILSFEDANRAMQTGVTGIMIARGALLKPWLFTEIKEQRHWDISSSERLDILRDFTNYGLEH WGSDTQGVEKTRRFLLEWLSFLCRYVPVGLLERLPQRINERPPYYLGRDYLETLMASQKAADWIRISEML LGPVPPSFAFLPKHKANAYK SGPTRTRRLE - GFP Tag - V |
| Restriction Sites |
SgfI-RsrII
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_020175 |
| ORF Size | 1950 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_020175.1, NP_064560.1 |
| RefSeq Size | 2078 bp |
| RefSeq ORF | 1953 bp |
| Locus ID | 56931 |
| UniProt ID | Q96G46 |
| Domains | zf-CCCH, Dus |
| Gene Summary | Catalyzes the synthesis of dihydrouridine, a modified base found in the D-loop of most tRNAs.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC203380 | DUS3L (Myc-DDK-tagged)-Human dihydrouridine synthase 3-like (S. cerevisiae) (DUS3L), transcript variant 1 |
CNY 4,560.00 |
|
| RC203380L3 | Lenti ORF clone of Human dihydrouridine synthase 3-like (S. cerevisiae) (DUS3L), transcript variant 1, Myc-DDK-tagged |
CNY 6,460.00 |
|
| RC203380L4 | Lenti ORF clone of Human dihydrouridine synthase 3-like (S. cerevisiae) (DUS3L), transcript variant 1, mGFP tagged |
CNY 6,460.00 |
|
| SC100465 | DUS3L (untagged)-Human dihydrouridine synthase 3-like (S. cerevisiae) (DUS3L), transcript variant 1 |
CNY 7,220.00 |
|
| SC321152 | DUS3L (untagged)-Human dihydrouridine synthase 3-like (S. cerevisiae) (DUS3L), transcript variant 1 |
CNY 4,848.00 |
