Tbp7 (PSMC4) (NM_153001) Human Tagged ORF Clone
CAT#: RG203161
- TrueORF®
PSMC4 (tGFP-tagged) - Human proteasome (prosome, macropain) 26S subunit, ATPase, 4 (PSMC4), transcript variant 2
ORF Plasmid: DDK
"NM_153001" in other vectors (6)
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CNY 5,256.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | TurboGFP |
Synonyms | MIP224; RPT3; S6; TBP-7; TBP7 |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RG203161 representing NM_153001
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGGAGATAGGCATCTTGGTGGAGAAGGCTCAGGATGAGATCCCAGCACTGTCCGTGTCCCGGCCCC AGACCGGCCTGTCCTTCCTGGGCCCTGAGCCTGAGGACCTGGAGGACCTGTACAGCCGCTACAAGGAGGA GGTGAAGCGAATCCAAAGCATCCCGCTGGTCATCGGACAATTTCTGGAGGCTGTGGATCAGAATACAGCC ATCGTGGGCTCTACCACAGGCTCCAACTATTATGTGCGCATCCTGAGCACCATCGATCGGGAGCTGCTCA AGCCCAACGCCTCAGTGGCCCTCCACAAGCACAGCAATGCACTGGTGGACGTGCTGCCCCCCGAAGCCGA CAGCAGCATCATGATGCTCACCTCAGACCAGAAGCCAGATGTGATGTACGCGGACATCGGAGGCATGGAC ATCCAGAAGCAGGAGGTGCGGGAGGCCGTGGAGCTCCCGCTCACGCATTTCGAGCTCTACAAGCAGATCG GCATCGATCCCCCCCGAGGCGTCCTCATGTATGGCCCACCTGGCTGTGGGAAGACCATGTTGGCAAAGGC GGTGGCACATCACACAACAGCTGCATTCATCCGGGTCGTGGGCTCGGAGTTTGTACAGAAGTATCTGGGT GAGGGCCCCCGCATGGTCCGGGATGTGTTCCGCCTGGCCAAGGAGAATGCACCTGCCATCATCTTCATAG ACGAGATTGATGCCATCGCCACCAAGAGATTCGATGCTCAGACAGGGGCCGACAGGGAGGTTCAGAGGAT CCTGCTGGAGCTGCTGAATCAGATGGATGGATTTGATCAGAATGTCAATGTCAAGGTAATCATGGCCACA AACAGAGCAGACACCCTGGATCCGGCCCTGCTACGGCCAGGACGGCTGGACCGTAAAATTGAATTTCCAC TTCCTGACCGCCGCCAGAAGAGATTGATTTTCTCCACTATCACTAGCAAGATGAACCTCTCTGAGGAGGT TGACTTGGAAGACTATGTGGCCCGGCCAGATAAGATTTCAGGAGCTGATATTAACTCCATCTGTCAGGAG AGTGGAATGTTGGCTGTCCGTGAAAACCGCTACATTGTCCTGGCCAAGGACTTCGAGAAAGCATACAAGA CTGTCATCAAGAAGGACGAGCAGGAGCATGAGTTTTACAAG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG203161 representing NM_153001
Red=Cloning site Green=Tags(s) MEEIGILVEKAQDEIPALSVSRPQTGLSFLGPEPEDLEDLYSRYKEEVKRIQSIPLVIGQFLEAVDQNTA IVGSTTGSNYYVRILSTIDRELLKPNASVALHKHSNALVDVLPPEADSSIMMLTSDQKPDVMYADIGGMD IQKQEVREAVELPLTHFELYKQIGIDPPRGVLMYGPPGCGKTMLAKAVAHHTTAAFIRVVGSEFVQKYLG EGPRMVRDVFRLAKENAPAIIFIDEIDAIATKRFDAQTGADREVQRILLELLNQMDGFDQNVNVKVIMAT NRADTLDPALLRPGRLDRKIEFPLPDRRQKRLIFSTITSKMNLSEEVDLEDYVARPDKISGADINSICQE SGMLAVRENRYIVLAKDFEKAYKTVIKKDEQEHEFYK TRTRPLE - GFP Tag - V |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_153001 |
ORF Size | 1161 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_153001.2 |
RefSeq Size | 1363 bp |
RefSeq ORF | 1164 bp |
Locus ID | 5704 |
UniProt ID | P43686 |
Domains | AAA, AAA |
Protein Families | Druggable Genome |
Protein Pathways | Proteasome |
Gene Summary | The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. This gene encodes a member of the triple-A family of ATPases that is a component of the 19S regulatory subunit and plays a role in 26S proteasome assembly. The encoded protein interacts with gankyrin, a liver oncoprotein, and may also play a role in Parkinson's disease through interactions with synphilin-1. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Jul 2012] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC203161 | PSMC4 (Myc-DDK-tagged)-Human proteasome (prosome, macropain) 26S subunit, ATPase, 4 (PSMC4), transcript variant 2 |
CNY 3,656.00 |
|
RC203161L1 | Lenti ORF clone of Human proteasome (prosome, macropain) 26S subunit, ATPase, 4 (PSMC4), transcript variant 2, Myc-DDK-tagged |
CNY 6,056.00 |
|
RC203161L2 | Lenti ORF clone of Human proteasome (prosome, macropain) 26S subunit, ATPase, 4 (PSMC4), transcript variant 2, mGFP tagged |
CNY 5,890.00 |
|
RC203161L3 | Lenti ORF clone of Human proteasome (prosome, macropain) 26S subunit, ATPase, 4 (PSMC4), transcript variant 2, Myc-DDK-tagged |
CNY 5,890.00 |
|
RC203161L4 | Lenti ORF clone of Human proteasome (prosome, macropain) 26S subunit, ATPase, 4 (PSMC4), transcript variant 2, mGFP tagged |
CNY 5,890.00 |
|
SC109643 | PSMC4 (untagged)-Human proteasome (prosome, macropain) 26S subunit, ATPase, 4 (PSMC4), transcript variant 2 |
CNY 7,220.00 |