G3BP (G3BP1) (NM_005754) Human Tagged ORF Clone
CAT#: RG202692
- TrueORF®
G3BP1 (tGFP-tagged) - Human GTPase activating protein (SH3 domain) binding protein 1 (G3BP1), transcript variant 1
ORF Plasmid: DDK
"NM_005754" in other vectors (7)
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CNY 4,370.00
Cited in 1 publication. |
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | TurboGFP |
Synonyms | G3BP; HDH-VIII |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RG202692 representing NM_005754
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGTGATGGAGAAGCCTAGTCCCCTGCTGGTCGGGCGGGAATTTGTGAGACAGTATTACACACTGCTGA ACCAGGCCCCAGACATGCTGCATAGATTTTATGGAAAGAACTCTTCTTATGTCCATGGGGGATTGGATTC AAATGGAAAGCCAGCAGATGCAGTCTACGGACAGAAAGAAATCCACAGGAAAGTGATGTCACAAAACTTC ACCAACTGCCACACCAAGATTCGCCATGTTGATGCTCATGCCACGCTAAATGATGGTGTGGTAGTCCAGG TGATGGGGCTTCTCTCTAACAACAACCAGGCTTTGAGGAGATTCATGCAAACGTTTGTCCTTGCTCCTGA GGGGTCTGTTGCAAATAAATTCTATGTTCACAATGATATCTTCAGATACCAAGATGAGGTCTTTGGTGGG TTTGTCACTGAGCCTCAGGAGGAGTCTGAAGAAGAAGTAGAGGAACCTGAAGAAAGACAGCAAACACCTG AGGTGGTACCTGATGATTCTGGAACTTTCTATGATCAGGCAGTTGTCAGTAATGACATGGAAGAACATTT AGAGGAGCCTGTTGCTGAACCAGAGCCTGATCCTGAACCAGAACCAGAACAAGAACCTGTATCTGAAATC CAAGAGGAAAAGCCTGAGCCAGTATTAGAAGAAACTGCCCCTGAGGATGCTCAGAAGAGTTCTTCTCCAG CACCTGCAGACATAGCTCAGACAGTACAGGAAGACTTGAGGACATTTTCTTGGGCATCTGTGACCAGTAA GAATCTTCCACCCAGTGGAGCTGTTCCAGTTACTGGGATACCACCTCATGTTGTTAAAGTACCAGCTTCA CAGCCCCGTCCAGAGTCTAAGCCTGAATCTCAGATTCCACCACAAAGACCTCAGCGGGATCAAAGAGTGC GAGAACAACGAATAAATATTCCTCCCCAAAGGGGACCCAGACCAATCCGTGAGGCTGGTGAGCAAGGTGA CATTGAACCCCGAAGAATGGTGAGACACCCTGACAGTCACCAACTCTTCATTGGCAACCTGCCTCATGAA GTGGACAAATCAGAGCTTAAAGATTTCTTTCAAAGTTATGGAAACGTGGTGGAGTTGCGCATTAACAGTG GTGGGAAATTACCCAATTTTGGTTTTGTTGTGTTTGATGATTCTGAGCCTGTTCAGAAAGTCCTTAGCAA CAGGCCCATCATGTTCAGAGGTGAGGTCCGTCTGAATGTCGAAGAGAAGAAGACTCGAGCTGCCAGGGAA GGCGACCGACGAGATAATCGCCTTCGGGGACCTGGAGGCCCTCGAGGTGGGCTGGGTGGTGGAATGAGAG GCCCTCCCCGTGGAGGCATGGTGCAGAAACCAGGATTTGGAGTGGGAAGGGGGCTTGCGCCACGGCAG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG202692 representing NM_005754
Red=Cloning site Green=Tags(s) MVMEKPSPLLVGREFVRQYYTLLNQAPDMLHRFYGKNSSYVHGGLDSNGKPADAVYGQKEIHRKVMSQNF TNCHTKIRHVDAHATLNDGVVVQVMGLLSNNNQALRRFMQTFVLAPEGSVANKFYVHNDIFRYQDEVFGG FVTEPQEESEEEVEEPEERQQTPEVVPDDSGTFYDQAVVSNDMEEHLEEPVAEPEPDPEPEPEQEPVSEI QEEKPEPVLEETAPEDAQKSSSPAPADIAQTVQEDLRTFSWASVTSKNLPPSGAVPVTGIPPHVVKVPAS QPRPESKPESQIPPQRPQRDQRVREQRINIPPQRGPRPIREAGEQGDIEPRRMVRHPDSHQLFIGNLPHE VDKSELKDFFQSYGNVVELRINSGGKLPNFGFVVFDDSEPVQKVLSNRPIMFRGEVRLNVEEKKTRAARE GDRRDNRLRGPGGPRGGLGGGMRGPPRGGMVQKPGFGVGRGLAPRQ TRTRPLE - GFP Tag - V |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_005754 |
ORF Size | 1398 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_005754.3 |
RefSeq Size | 2855 bp |
RefSeq ORF | 1401 bp |
Locus ID | 10146 |
UniProt ID | Q13283 |
Domains | RRM, NTF2 |
Protein Families | Druggable Genome |
Gene Summary | This gene encodes one of the DNA-unwinding enzymes which prefers partially unwound 3'-tailed substrates and can also unwind partial RNA/DNA and RNA/RNA duplexes in an ATP-dependent fashion. This enzyme is a member of the heterogeneous nuclear RNA-binding proteins and is also an element of the Ras signal transduction pathway. It binds specifically to the Ras-GTPase-activating protein by associating with its SH3 domain. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of some of these variants has not been determined. [provided by RefSeq, Jul 2008] |
Citations (1)
The use of this cDNA Clones has been cited in the following citations: |
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A stress-induced response complex (SIRC) shuttles miRNAs, siRNAs, and oligonucleotides to the nucleus
,Castanotto, D;Zhang, X;Alluin, J;Zhang, X;Rüger, J;Armstrong, B;Rossi, J;Riggs, A;Stein, CA;,
Proc. Natl. Acad. Sci. U.S.A.
,PubMed ID 29866826
[G3BP1]
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Documents
Product Manuals |
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SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC202692 | G3BP1 (Myc-DDK-tagged)-Human GTPase activating protein (SH3 domain) binding protein 1 (G3BP1), transcript variant 1 |
CNY 3,656.00 |
|
RC202692L1 | Lenti ORF clone of Human GTPase activating protein (SH3 domain) binding protein 1 (G3BP1), transcript variant 1, Myc-DDK-tagged |
CNY 6,056.00 |
|
RC202692L2 | Lenti ORF clone of Human GTPase activating protein (SH3 domain) binding protein 1 (G3BP1), transcript variant 1, mGFP tagged |
CNY 6,056.00 |
|
RC202692L3 | Lenti ORF clone of Human GTPase activating protein (SH3 domain) binding protein 1 (G3BP1), transcript variant 1, Myc-DDK-tagged |
CNY 6,056.00 |
|
RC202692L4 | Lenti ORF clone of Human GTPase activating protein (SH3 domain) binding protein 1 (G3BP1), transcript variant 1, mGFP tagged |
CNY 6,056.00 |
|
SC116551 | G3BP1 (untagged)-Human GTPase activating protein (SH3 domain) binding protein 1 (G3BP1), transcript variant 1 |
CNY 5,488.00 |
|
SC321062 | G3BP1 (untagged)-Human GTPase activating protein (SH3 domain) binding protein 1 (G3BP1), transcript variant 1 |
CNY 5,488.00 |