BTN3A3 (NM_197974) Human Tagged ORF Clone
CAT#: RG202536
- TrueORF®
BTN3A3 (tGFP-tagged) - Human butyrophilin, subfamily 3, member A3 (BTN3A3), transcript variant 2
ORF Plasmid: DDK
"NM_197974" in other vectors (4)
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CNY 5,130.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | TurboGFP |
Synonyms | BTF3; BTN3.3 |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RG202536 representing NM_197974
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGTGGGTGAAGACGCTGATCTGCCCTGTCACCTGTTCCCGACCATGAGTGCAGAGACCATGGAGCTGA GGTGGGTGAGTTCCAGCCTAAGGCAGGTGGTGAACGTGTATGCAGATGGAAAGGAAGTGGAAGACAGGCA GAGTGCACCGTATCGAGGGAGAACTTCGATTCTGCGGGATGGCATCACTGCAGGGAAGGCTGCTCTCCGA ATACACAACGTCACAGCCTCTGACAGTGGAAAGTACTTGTGTTATTTCCAAGATGGTGACTTCTACGAAA AAGCCCTGGTGGAGCTGAAGGTTGCAGCATTGGGTTCTGATCTTCACATTGAAGTGAAGGGTTATGAGGA TGGAGGGATCCATCTGGAGTGCAGGTCCACTGGCTGGTACCCCCAACCCCAAATAAAGTGGAGCGACACC AAGGGAGAGAACATCCCGGCTGTGGAAGCACCTGTGGTTGCAGATGGAGTGGGCCTGTATGCAGTAGCAG CATCTGTGATCATGAGAGGCAGCTCTGGTGGGGGTGTATCCTGCATCATCAGAAATTCCCTCCTCGGCCT GGAAAAGACAGCCAGCATATCCATCGCAGACCCCTTCTTCAGGAGCGCCCAGCCCTGGATCGCGGCCCTG GCAGGGACCCTGCCTATCTCGTTGCTGCTTCTCGCAGGAGCCAGTTACTTCTTGTGGAGACAACAGAAGG AAAAAATTGCTCTGTCCAGGGAGACAGAAAGAGAGCGAGAGATGAAAGAAATGGGATACGCTGCAACAGA GCAAGAAATAAGCCTAAGAGAGTGGAGGAAAATCCAGTACATGGCTCGTGGAGAGAAGTCTTTGGCCTAT CATGAATGGAAAATGGCCCTCTTCAAACCTGCGGATGTGATTCTGGATCCAGACACGGCAAACGCCATCC TCCTTGTTTCTGAGGACCAGAGGAGTGTGCAGCGTGCTGAAGAGCCGCGGGATCTGCCAGACAACCCTGA GAGATTTGAATGGCGTTACTGTGTCCTTGGCTGTGAAAACTTCACATCAGGGAGACATTACTGGGAGGTG GAAGTGGGGGACAGAAAAGAGTGGCATATTGGGGTATGTAGTAAGAACGTGGAGAGGAAAAAAGGTTGGG TCAAAATGACACCGGAGAACGGATACTGGACTATGGGCCTGACTGATGGGAATAAGTATCGGGCTCTCAC TGAGCCCAGAACCAACCTGAAACTTCCTGAGCCTCCTAGGAAAGTGGGGATCTTCCTGGACTATGAGACT GGAGAGATCTCGTTCTATAATGCCACAGATGGATCTCATATCTACACCTTTCCGCACGCCTCTTTCTCTG AGCCTCTATATCCTGTTTTCAGAATTTTGACCTTGGAGCCCACTGCCCTGACCATTTGCCCAATACCAAA AGAAGTAGAGAGTTCCCCCGATCCTGACCTAGTGCCTGATCATTCCCTGGAGACACCACTGACCCCGGGC TTAGCTAATGAAAGTGGGGAGCCTCAGGCTGAAGTAACATCTCTGCTTCTCCCTGCCCACCCTGGAGCTG AGGTCTCCCCTTCTGCAACAACCAATCAGAACCATAAGCTACAGGCACGCACTGAAGCACTTTAC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG202536 representing NM_197974
Red=Cloning site Green=Tags(s) MVGEDADLPCHLFPTMSAETMELRWVSSSLRQVVNVYADGKEVEDRQSAPYRGRTSILRDGITAGKAALR IHNVTASDSGKYLCYFQDGDFYEKALVELKVAALGSDLHIEVKGYEDGGIHLECRSTGWYPQPQIKWSDT KGENIPAVEAPVVADGVGLYAVAASVIMRGSSGGGVSCIIRNSLLGLEKTASISIADPFFRSAQPWIAAL AGTLPISLLLLAGASYFLWRQQKEKIALSRETEREREMKEMGYAATEQEISLREWRKIQYMARGEKSLAY HEWKMALFKPADVILDPDTANAILLVSEDQRSVQRAEEPRDLPDNPERFEWRYCVLGCENFTSGRHYWEV EVGDRKEWHIGVCSKNVERKKGWVKMTPENGYWTMGLTDGNKYRALTEPRTNLKLPEPPRKVGIFLDYET GEISFYNATDGSHIYTFPHASFSEPLYPVFRILTLEPTALTICPIPKEVESSPDPDLVPDHSLETPLTPG LANESGEPQAEVTSLLLPAHPGAEVSPSATTNQNHKLQARTEALY TRTRPLE - GFP Tag - V |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_197974 |
ORF Size | 1605 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_197974.3 |
RefSeq Size | 2927 bp |
RefSeq ORF | 1608 bp |
Locus ID | 10384 |
UniProt ID | O00478 |
Protein Families | Druggable Genome, Transmembrane |
Gene Summary | The butyrophilin (BTN) genes are a group of major histocompatibility complex (MHC)-associated genes that encode type I membrane proteins with 2 extracellular immunoglobulin (Ig) domains and an intracellular B30.2 (PRYSPRY) domain. Three subfamilies of human BTN genes are located in the MHC class I region: the single-copy BTN1A1 gene (MIM 601610) and the BTN2 (e.g., BTN2A1; MIM 613590) and BTN3 (e.g., BNT3A3) genes, which have undergone tandem duplication, resulting in 3 copies of each (summary by Smith et al., 2010 [PubMed 20208008]).[supplied by OMIM, Nov 2010] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC202536 | BTN3A3 (Myc-DDK-tagged)-Human butyrophilin, subfamily 3, member A3 (BTN3A3), transcript variant 2 |
CNY 4,660.00 |
|
RC202536L3 | Lenti-ORF clone of BTN3A3 (Myc-DDK-tagged)-Human butyrophilin, subfamily 3, member A3 (BTN3A3), transcript variant 2 |
CNY 6,560.00 |
|
RC202536L4 | Lenti-ORF clone of BTN3A3 (mGFP-tagged)-Human butyrophilin, subfamily 3, member A3 (BTN3A3), transcript variant 2 |
CNY 6,560.00 |
|
SC107714 | BTN3A3 (untagged)-Human butyrophilin, subfamily 3, member A3 (BTN3A3), transcript variant 2 |
CNY 4,040.00 |