ARFGAP3 (NM_014570) Human Tagged ORF Clone
CAT#: RG202392
- TrueORF®
ARFGAP3 (tGFP-tagged) - Human ADP-ribosylation factor GTPase activating protein 3 (ARFGAP3), transcript variant 1
ORF Plasmid: DDK
"NM_014570" in other vectors (5)
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CNY 5,440.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | TurboGFP |
Synonyms | ARFGAP1 |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RG202392 representing NM_014570
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGGGACCCCAGCAAGCAGGACATCTTGACCATCTTCAAGCGCCTCCGCTCGGTGCCCACTAACAAGG TGTGTTTTGATTGTGGTGCCAAAAATCCCAGCTGGGCAAGCATAACCTATGGAGTGTTCCTTTGCATTGA TTGCTCAGGGTCCCACCGGTCACTTGGTGTTCACTTGAGTTTTATTCGATCTACAGAGTTGGATTCCAAC TGGTCATGGTTTCAGTTGCGATGCATGCAAGTCGGAGGAAACGCTAGTGCATCTTCCTTTTTTCATCAAC ATGGGTGTTCCACCAATGACACCAATGCCAAGTACAACAGTCGTGCTGCTCAGCTCTATAGGGAGAAAAT CAAATCGCTCGCCTCTCAAGCAACACGGAAGCATGGCACTGATCTGTGGCTTGATAGTTGTGTGGTTCCA CCTTTGTCCCCTCCACCAAAGGAGGAAGATTTTTTTGCCTCTCACGTTTCTCCTGAGGTGAGTGACACAG CGTGGGCATCAGCAATAGCAGAACCATCTTCTTTAACATCAAGGCCTGTGGAAACCACTTTGGAAAATAA TGAAGGTGGACAAGAGCAAGGACCAAGTGTGGAAGGTCTTAATGTACCAACAAAGGCTACTTTAGAGGTA TCCTCTATCATAAAAAAGAAACCAAATCAAGCTAAAAAAGGCCTTGGGGCCAAAAAAGGAAGTTTGGGAG CTCAGAAACTGGCAAACACATGCTTTAATGAAATTGAAAAACAAGCTCAAGCTGCGGATAAAATGAAGGA GCAGGAAGACCTGGCCAAGGTGGTATCTAAAGAAGAATCAATTGTTTCATCATTACGATTAGCCTATAAG GATCTTGAAATTCAAATGAAGAAAGACGAAAAGATGAACATTAGTGGCAAAAAAAATGTTGACTCAGACA GACTCGGCATGGGATTTGGAAATTGCAGAAGTGTTATTTCACATTCAGTGACTTCAGATATGCAGACCAT AGAGCAGGAATCACCCATTATGGCAAAACCAAGAAAAAAGTATAATGATGACAGTGACGATTCATATTTT ACTTCCAGCTCAAGGTACTTTGACGAGCCAGTGGAGTTAAGGAGCAGTTCTTTCTCTAGCTGGGATGACA GTTCAGATTCCTATTGGAAAAAAGAGACCAGCAAAGATACTGAAACAGTTCTGAAAACCACAGGCTATTC AGACAGACCTACTGCTCGCCGCAAGCCAGATTATGAGCCAGTTGAAAATACAGATGAGGCCCAGAAGAAG TTTGGCAATGTCAAGGCCATTTCATCAGATATGTATTTTGGAAGACAATCCCAGGCTGATTATGAGACCA GGGCCCGCCTAGAGAGGCTGTCAGCAAGTTCCTCCATAAGCTCGGCTGATCTGTTCGAGGAGCCGAGGAA GCAGCCAGCAGGGAACTACAGCCTGTCCAGCGTGCTGCCCAACGCCCCCGACATGGCGCAGTTCAAGCAG GGAGTGAGATCGGTTGCTGGAAAACTCTCCGTCTTTGCTAATGGAGTCGTGACTTCAATTCAGGATCGCT ACGGTTCT ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG202392 representing NM_014570
Red=Cloning site Green=Tags(s) MGDPSKQDILTIFKRLRSVPTNKVCFDCGAKNPSWASITYGVFLCIDCSGSHRSLGVHLSFIRSTELDSN WSWFQLRCMQVGGNASASSFFHQHGCSTNDTNAKYNSRAAQLYREKIKSLASQATRKHGTDLWLDSCVVP PLSPPPKEEDFFASHVSPEVSDTAWASAIAEPSSLTSRPVETTLENNEGGQEQGPSVEGLNVPTKATLEV SSIIKKKPNQAKKGLGAKKGSLGAQKLANTCFNEIEKQAQAADKMKEQEDLAKVVSKEESIVSSLRLAYK DLEIQMKKDEKMNISGKKNVDSDRLGMGFGNCRSVISHSVTSDMQTIEQESPIMAKPRKKYNDDSDDSYF TSSSRYFDEPVELRSSSFSSWDDSSDSYWKKETSKDTETVLKTTGYSDRPTARRKPDYEPVENTDEAQKK FGNVKAISSDMYFGRQSQADYETRARLERLSASSSISSADLFEEPRKQPAGNYSLSSVLPNAPDMAQFKQ GVRSVAGKLSVFANGVVTSIQDRYGS TRTRPLE - GFP Tag - V |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_014570 |
ORF Size | 1548 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_014570.2 |
RefSeq Size | 2725 bp |
RefSeq ORF | 1551 bp |
Locus ID | 26286 |
UniProt ID | Q9NP61 |
Domains | ArfGap |
Protein Pathways | Endocytosis |
Gene Summary | The protein encoded by this gene is a GTPase-activating protein (GAP) that associates with the Golgi apparatus and regulates the early secretory pathway of proteins. The encoded protein promotes hydrolysis of ADP-ribosylation factor 1 (ARF1)-bound GTP, which is required for the dissociation of coat proteins from Golgi-derived membranes and vesicles. Dissociation of the coat proteins is a prerequisite for the fusion of these vesicles with target compartments. The activity of this protein is sensitive to phospholipids. Multiple transcript variants encoding different isoforms have been found for this gene. This gene was originally known as ARFGAP1, but that is now the name of a related but different gene. [provided by RefSeq, Nov 2008] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC202392 | ARFGAP3 (Myc-DDK-tagged)-Human ADP-ribosylation factor GTPase activating protein 3 (ARFGAP3), transcript variant 1 |
CNY 3,840.00 |
|
RC202392L3 | Lenti ORF clone of Human ADP-ribosylation factor GTPase activating protein 3 (ARFGAP3), transcript variant 1, Myc-DDK-tagged |
CNY 5,890.00 |
|
RC202392L4 | Lenti ORF clone of Human ADP-ribosylation factor GTPase activating protein 3 (ARFGAP3), transcript variant 1, mGFP tagged |
CNY 5,890.00 |
|
SC114939 | ARFGAP3 (untagged)-Human ADP-ribosylation factor GTPase activating protein 3 (ARFGAP3), transcript variant 1 |
CNY 3,848.00 |
|
SC324381 | ARFGAP3 (untagged)-Human ADP-ribosylation factor GTPase activating protein 3 (ARFGAP3), transcript variant 1 |
CNY 3,848.00 |
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