APC16 (ANAPC16) (NM_173473) Human Tagged ORF Clone

CAT#: RG202340

  • TrueORF®

ANAPC16 (tGFP-tagged) - Human anaphase promoting complex subunit 16 (ANAPC16)

ORF Plasmid: DDK tGFP



  "NM_173473" in other vectors (6)

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CNY 2,800.00

CNY 4,370.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (4)
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Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
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Specifications

Product Data
Type Human Tagged ORF Clone
Tag TurboGFP
Synonyms APC16; bA570G20.3; C10orf104; CENP-27; MSAG
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RG202340 representing NM_173473
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCTGCTTCATCATCATCCTCCTCAGCTGGTGGGGTCAGTGGAAGTTCTGTCACTGGATCTGGTTTCA
GTGTCTCAGACCTTGCCCCACCACGGAAAGCCCTTTTCACCTACCCCAAAGGAGCTGGAGAGATGTTAGA
AGATGGCTCTGAGAGATTCCTCTGCGAATCTGTTTTTAGCTATCAAGTGGCATCCACGCTTAAACAGGTG
AAACATGATCAGCAAGTTGCTCGGATGGAAAAACTAGCTGGTTTGGTAGAAGAGCTGGAGGCTGACGAGT
GGCGGTTTAAGCCCATCGAGCAGCTGCTGGGATTCACCCCCTCTTCAGGT


ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
>RG202340 representing NM_173473
Red=Cloning site Green=Tags(s)

MAASSSSSSAGGVSGSSVTGSGFSVSDLAPPRKALFTYPKGAGEMLEDGSERFLCESVFSYQVASTLKQV
KHDQQVARMEKLAGLVEELEADEWRFKPIEQLLGFTPSSG

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_173473
ORF Size 330 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_173473.4
RefSeq Size 3277 bp
RefSeq ORF 333 bp
Locus ID 119504
UniProt ID Q96DE5
Gene Summary Component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. The APC/C complex acts by mediating ubiquitination and subsequent degradation of target proteins: it mainly mediates the formation of 'Lys-11'-linked polyubiquitin chains and, to a lower extent, the formation of 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains.[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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