DIMT1L (DIMT1) (NM_014473) Human Tagged ORF Clone

CAT#: RG202326

  • TrueORF®

DIMT1 (tGFP-tagged) - Human DIM1 dimethyladenosine transferase 1-like (S. cerevisiae) (DIMT1L)

ORF Plasmid: DDK tGFP



  "NM_014473" in other vectors (7)

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CNY 4,000.00

CNY 4,370.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (5)
pCMV6-AC-GFP, mammalian vector with C-terminal tGFP tag, 10ug
    • 10 ug

CNY 6,080.00


Mouse monoclonal turboGFP antibody, clone OTI2H8
    • 100 ul

CNY 1,000.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


DIMT1L mouse monoclonal antibody,clone OTI6E7
    • 100 ul

CNY 1,999.00
CNY 2,700.00

Specifications

Product Data
Type Human Tagged ORF Clone
Tag TurboGFP
Synonyms DIM1; DIMT1L; HSA9761; HUSSY5
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RG202326 representing NM_014473
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCCGAAGGTCAAGTCGGGGGCCATCGGCCGCCGCCGCGGGCGGCAGGAGCAGCGCCGGGAGCTGAAGA
GCGCTGGAGGACTCATGTTCAACACGGGGATTGGGCAGCACATTTTGAAAAATCCTCTCATTATTAACAG
CATTATCGATAAGGCTGCCTTAAGACCAACTGATGTAGTGCTGGAAGTTGGACCTGGAACTGGCAACATG
ACTGTAAAGTTGTTAGAAAAGGCAAAAAAGGTTGTTGCTTGTGAACTTGACCCAAGGCTAGTAGCTGAAC
TTCACAAAAGAGTTCAGGGCACGCCTGTGGCCAGCAAACTTCAAGTACTGGTGGGTGATGTGCTGAAAAC
AGATTTGCCATTCTTTGATACTTGTGTGGCAAATTTGCCTTATCAGATCTCTTCGCCTTTTGTCTTCAAG
CTGTTGCTACATCGACCTTTTTTCAGGTGTGCTATACTTATGTTTCAAAGAGAATTTGCCCTCCGACTGG
TTGCAAAACCTGGAGATAAGTTATACTGCAGACTCTCAATTAATACACAGCTGTTGGCACGTGTGGACCA
TCTAATGAAAGTGGGAAAGAATAACTTCAGACCACCGCCCAAGGTGGAATCCAGTGTTGTAAGGATAGAA
CCTAAGAATCCACCACCACCCATCAATTTTCAGGAATGGGATGGTCTAGTAAGGATAACCTTTGTTAGGA
AAAACAAGACACTCTCTGCTGCATTTAAATCAAGTGCAGTGCAACAACTCTTGGAAAAAAACTACAGAAT
TCACTGTTCAGTCCATAATATTATAATACCAGAAGATTTCAGCATAGCAGATAAAATACAGCAAATCCTA
ACCAGCACAGGTTTTAGTGACAAACGGGCCCGTTCCATGGACATAGATGACTTCATCAGATTGCTACATG
GATTCAACGCAGAAGGTATTCATTTTTCC


ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
>RG202326 representing NM_014473
Red=Cloning site Green=Tags(s)

MPKVKSGAIGRRRGRQEQRRELKSAGGLMFNTGIGQHILKNPLIINSIIDKAALRPTDVVLEVGPGTGNM
TVKLLEKAKKVVACELDPRLVAELHKRVQGTPVASKLQVLVGDVLKTDLPFFDTCVANLPYQISSPFVFK
LLLHRPFFRCAILMFQREFALRLVAKPGDKLYCRLSINTQLLARVDHLMKVGKNNFRPPPKVESSVVRIE
PKNPPPPINFQEWDGLVRITFVRKNKTLSAAFKSSAVQQLLEKNYRIHCSVHNIIIPEDFSIADKIQQIL
TSTGFSDKRARSMDIDDFIRLLHGFNAEGIHFS

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_014473
ORF Size 939 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_014473.1
RefSeq Size 1557 bp
RefSeq ORF 942 bp
Locus ID 27292
UniProt ID Q9UNQ2
Domains rADc
Protein Families Druggable Genome
Gene Summary The protein encoded by this gene is a methyltransferase that is responsible for dimethylation of adjacent adenosines near the 18S rRNA decoding site. The encoded protein is essential for ribosome biogenesis, although its catalytic activity is not involved in the process. The yeast ortholog of this protein functions in the cytoplasm while this protein functions in the nucleus. [provided by RefSeq, Jan 2017]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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