TMEM159 (NM_020422) Human Tagged ORF Clone
CAT#: RG202226
- TrueORF®
TMEM159 (tGFP-tagged) - Human transmembrane protein 159 (TMEM159)
ORF Plasmid: DDK
"NM_020422" in other vectors (4)
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CNY 3,400.00
Cited in 1 publication. |
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | TurboGFP |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RG202226 representing NM_020422
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCAAAAGAGGAGCCCCAGAGTATCTCAAGGGACTTGCAGGAACTGCAGAAGAAGCTGTCTCTGCTGA TAGACTCCTTCCAGAATAACTCAAAGGTGGTGGCCTTTATGAAGTCTCCAGTGGGTCAGTACTTGGACAG CCATCCGTTTCTGGCCTTCACCTTGCTGGTGTTCATTGTCATGTCGGCCGTTCCTGTTGGATTCTTCCTG CTCATCGTGGTGCTTACCACCCTGGCTGCTCTGCTGGGGGTCATAATATTGGAAGGATTGGTCATCTCTG TGGGTGGCTTCTCACTGCTCTGCATCCTCTGTGGTTTGGGCTTCGTATCACTCGCCATGTCGGGGATGAT GATAGCATCTTATGTAGTGGTCTCCAGCCTCATCAGCTGCTGGTTTTCTCCCAGGCCACTGACACAGCAA AACACCAGTTGTGACTTTCTGCCAGCCATGAAGTCTGCAGACTTCGAGGGGCTTTACCAGGAA ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG202226 representing NM_020422
Red=Cloning site Green=Tags(s) MAKEEPQSISRDLQELQKKLSLLIDSFQNNSKVVAFMKSPVGQYLDSHPFLAFTLLVFIVMSAVPVGFFL LIVVLTTLAALLGVIILEGLVISVGGFSLLCILCGLGFVSLAMSGMMIASYVVVSSLISCWFSPRPLTQQ NTSCDFLPAMKSADFEGLYQE TRTRPLE - GFP Tag - V |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_020422 |
ORF Size | 483 bp |
OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_020422.3, NP_065155.2 |
RefSeq Size | 1811 bp |
RefSeq ORF | 486 bp |
Locus ID | 57146 |
UniProt ID | Q96B96 |
Protein Families | Transmembrane |
Gene Summary | Plays an important role in the formation of lipid droplets (LD) which are storage organelles at the center of lipid and energy homeostasis (PubMed:31708432). In association with BSCL2/seipin, defines the sites of LD formation in the endoplasmic reticulum (PubMed:31708432).[UniProtKB/Swiss-Prot Function] |
Citations (1)
The use of this cDNA Clones has been cited in the following citations: |
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Promethin Is a Conserved Seipin Partner Protein
,Castro, IG;Eisenberg-Bord, M;Persiani, E;Rochford, JJ;Schuldiner, M;Bohnert, M;,
Cells
,PubMed ID 30901948
[TMEM159]
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Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC202226 | TMEM159 (Myc-DDK-tagged)-Human transmembrane protein 159 (TMEM159) |
CNY 1,800.00 |
|
RC202226L3 | Lenti ORF clone of Human transmembrane protein 159 (TMEM159), Myc-DDK-tagged |
CNY 5,890.00 |
|
RC202226L4 | Lenti ORF clone of Human transmembrane protein 159 (TMEM159), mGFP tagged |
CNY 5,890.00 |
|
SC113078 | TMEM159 (untagged)-Human transmembrane protein 159 (TMEM159) |
CNY 2,950.00 |
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