SF3B14 (SF3B6) (NM_016047) Human Tagged ORF Clone
CAT#: RG201866
- TrueORF®
SF3B14 (tGFP-tagged) - Human splicing factor 3B, 14 kDa subunit (SF3B14)
ORF Plasmid: DDK
"NM_016047" in other vectors (6)
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CNY 2,800.00
CNY 4,370.00
CNY 1,999.00
CNY 2,700.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | TurboGFP |
Synonyms | CGI-110; HSPC175; Ht006; P14; SAP14; SAP14a; SF3B14; SF3B14a |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RG201866 representing NM_016047
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGATGCAAGCGGCCAAGAGGGCGAACATTCGACTTCCACCTGAAGTAAATCGGATATTGTATATAA GAAATTTGCCATACAAAATCACAGCTGAAGAAATGTATGATATATTTGGGAAATATGGACCTATTCGTCA AATCAGAGTGGGGAACACACCTGAAACTAGAGGAACAGCTTATGTGGTCTATGAGGACATCTTTGATGCC AAGAATGCATGTGATCACCTATCGGGATTCAATGTTTGTAACAGATACCTTGTGGTTTTGTACTATAATG CCAACAGGGCATTTCAGAAGATGGACACAAAGAAGAAGGAGGAACAGTTGAAGCTTCTCAAGGAGAAATA TGGCATCAACACAGATCCACCAAAA ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG201866 representing NM_016047
Red=Cloning site Green=Tags(s) MAMQAAKRANIRLPPEVNRILYIRNLPYKITAEEMYDIFGKYGPIRQIRVGNTPETRGTAYVVYEDIFDA KNACDHLSGFNVCNRYLVVLYYNANRAFQKMDTKKKEEQLKLLKEKYGINTDPPK TRTRPLE - GFP Tag - V |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_016047 |
ORF Size | 375 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_016047.4 |
RefSeq Size | 783 bp |
RefSeq ORF | 378 bp |
Locus ID | 51639 |
UniProt ID | Q9Y3B4 |
Domains | RRM |
Protein Pathways | Spliceosome |
Gene Summary | This gene encodes a 14 kDa protein subunit of the splicing factor 3b complex. Splicing factor 3b associates with both the U2 and U11/U12 small nuclear ribonucleoprotein complexes (U2 snRNP) of spliceosomes. This 14 kDa protein interacts directly with subunit 1 of the splicing factor 3b complex. This 14 kDa protein also interacts directly with the adenosine that carries out the first transesterification step of splicing at the pre-mRNA branch site. [provided by RefSeq, Jul 2008] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC201866 | SF3B14 (Myc-DDK-tagged)-Human splicing factor 3B, 14 kDa subunit (SF3B14) |
CNY 1,200.00 |
|
RC201866L1 | Lenti ORF clone of Human splicing factor 3B, 14 kDa subunit (SF3B14), Myc-DDK-tagged |
CNY 3,600.00 |
|
RC201866L2 | Lenti ORF clone of Human splicing factor 3B, 14 kDa subunit (SF3B14), mGFP tagged |
CNY 5,890.00 |
|
RC201866L3 | Lenti ORF clone of Human splicing factor 3B, 14 kDa subunit (SF3B14), Myc-DDK-tagged |
CNY 5,890.00 |
|
RC201866L4 | Lenti ORF clone of Human splicing factor 3B, 14 kDa subunit (SF3B14), mGFP tagged |
CNY 5,890.00 |
|
SC107008 | SF3B14 (untagged)-Human splicing factor 3B, 14 kDa subunit (SF3B14) |
CNY 1,200.00 |