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Aconitase 1 (ACO1) (NM_002197) Human Tagged ORF Clone
CAT＃: RG201857
- TrueORF®
ACO1 (tGFP-tagged) - Human aconitase 1, soluble (ACO1)
ORF Plasmid: DDK
"NM_002197" in other vectors (7)
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CNY 8,112.00
Product images
CNY 1,000.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | ACONS; HEL60; IREB1; IREBP; IREBP1; IRP1 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG201857 representing NM_002197
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGCAACCCATTCGCACACCTTGCTGAGCCATTGGATCCTGTACAACCAGGAAAGAAATTCTTCAATT TGAATAAATTGGAGGATTCAAGATATGGGCGCTTACCATTTTCGATCAGAGTTCTTCTGGAAGCAGCCAT TCGGAATTGTGATGAGTTTTTGGTGAAGAAACAGGATATTGAAAATATTCTACATTGGAATGTCACGCAG CACAAGAACATAGAAGTGCCATTTAAGCCTGCTCGTGTCATCCTGCAGGACTTTACGGGTGTGCCCGCTG TGGTTGACTTTGCTGCAATGCGTGATGCTGTGAAAAAGTTAGGAGGAGATCCAGAGAAAATAAACCCTGT CTGCCCTGCTGATCTTGTAATAGATCATTCCATCCAGGTTGATTTCAACAGAAGGGCAGACAGTTTACAG AAGAATCAAGACCTGGAATTTGAAAGAAATAGAGAGCGATTTGAATTTTTAAAGTGGGGTTCCCAGGCTT TTCACAACATGCGGATTATTCCCCCTGGCTCAGGAATCATCCACCAGGTGAATTTGGAATATTTGGCAAG AGTGGTATTTGATCAGGATGGATATTATTACCCAGACAGCCTCGTGGGCACAGACTCGCACACTACCATG ATTGATGGCTTGGGCATTCTTGGTTGGGGTGTCGGTGGTATTGAAGCAGAAGCTGTCATGCTGGGTCAGC CAATCAGTATGGTGCTTCCTCAGGTGATTGGCTACAGGCTGATGGGGAAGCCCCACCCTCTGGTAACATC CACTGACATCGTGCTCACCATTACCAAGCACCTCCGCCAGGTTGGGGTAGTGGGCAAATTTGTCGAGTTC TTCGGGCCTGGAGTAGCCCAGTTGTCCATTGCTGACCGAGCTACGATTGCTAACATGTGTCCAGAGTACG GAGCAACTGCTGCCTTTTTCCCAGTTGATGAAGTTAGTATCACGTACCTGGTGCAAACAGGTCGTGATGA AGAAAAATTAAAGTATATTAAAAAATATCTTCAGGCTGTAGGAATGTTTCGAGATTTCAATGACCCTTCT CAAGACCCAGACTTCACCCAGGTTGTGGAATTAGATTTGAAAACAGTAGTGCCTTGCTGTAGTGGACCCA AAAGGCCTCAGGACAAAGTTGCTGTGTCCGACATGAAAAAGGACTTTGAGAGCTGCCTTGGAGCCAAGCA AGGATTTAAAGGATTCCAAGTTGCTCCTGAACATCATAATGACCATAAGACCTTTATCTATGATAACACT GAATTCACCCTTGCTCATGGTTCTGTGGTCATTGCTGCCATTACTAGCTGCACAAACACCAGTAATCCGT CTGTGATGTTAGGGGCAGGATTGTTAGCAAAGAAAGCTGTGGATGCTGGCCTGAACGTGATGCCTTACAT CAAAACTAGCCTGTCTCCTGGGAGTGGCGTGGTCACCTACTACCTACAAGAAAGCGGAGTCATGCCTTAT CTGTCTCAGCTTGGGTTTGACGTGGTGGGCTATGGCTGCATGACCTGCATTGGCAACAGTGGGCCTTTAC CTGAACCTGTGGTAGAAGCCATCACACAGGGAGACCTTGTAGCTGTTGGAGTACTATCTGGAAACAGGAA TTTTGAAGGTCGAGTTCACCCCAACACCCGGGCCAACTATTTAGCCTCTCCCCCCTTAGTAATAGCATAT GCAATTGCTGGAACCATCAGAATCGACTTTGAGAAAGAGCCATTGGGAGTAAATGCAAAGGGACAGCAGG TATTTCTGAAAGATATCTGGCCGACTAGAGACGAGATCCAGGCAGTGGAGCGTCAGTATGTCATCCCGGG GATGTTTAAGGAAGTCTATCAGAAAATAGAGACTGTGAATGAAAGCTGGAATGCCTTAGCAACCCCATCA GATAAGCTGTTTTTCTGGAATTCCAAATCTACGTATATCAAATCACCACCATTCTTTGAAAACCTGACTT TGGATCTTCAGCCCCCTAAATCTATAGTGGATGCCTATGTGCTGCTAAATTTGGGAGATTCGGTAACAAC TGACCACATCTCCCCAGCTGGAAATATTGCAAGAAACAGTCCTGCTGCTCGCTACTTAACTAACAGAGGC CTAACTCCACGAGAATTCAACTCCTATGGCTCCCGCCGAGGTAATGACGCCGTCATGGCACGGGGAACAT TTGCCAACATTCGCTTGTTAAACAGATTTTTGAACAAGCAGGCACCACAGACTATCCATCTGCCTTCTGG GGAAATCCTTGATGTGTTTGATGCTGCTGAGCGGTACCAGCAGGCAGGCCTTCCCCTGATCGTTCTGGCT GGCAAAGAGTACGGTGCAGGCAGCTCCCGAGACTGGGCAGCTAAGGGCCCTTTCCTGCTGGGAATCAAAG CCGTCCTGGCCGAGAGCTACGAGCGCATTCACCGCAGTAACCTGGTTGGGATGGGTGTGATCCCACTTGA ATATCTCCCTGGTGAGAATGCAGATGCCCTGGGGCTCACAGGGCAAGAACGATACACTATCATTATTCCA GAAAACCTCAAACCACAAATGAAAGTCCAGGTCAAGCTGGATACTGGCAAGACCTTCCAGGCTGTCATGA GGTTTGACACTGATGTGGAGCTCACTTATTTCCTCAACGGGGGCATCCTCAACTACATGATCCGCAAGAT GGCCAAG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG201857 representing NM_002197
Red=Cloning site Green=Tags(s) MSNPFAHLAEPLDPVQPGKKFFNLNKLEDSRYGRLPFSIRVLLEAAIRNCDEFLVKKQDIENILHWNVTQ HKNIEVPFKPARVILQDFTGVPAVVDFAAMRDAVKKLGGDPEKINPVCPADLVIDHSIQVDFNRRADSLQ KNQDLEFERNRERFEFLKWGSQAFHNMRIIPPGSGIIHQVNLEYLARVVFDQDGYYYPDSLVGTDSHTTM IDGLGILGWGVGGIEAEAVMLGQPISMVLPQVIGYRLMGKPHPLVTSTDIVLTITKHLRQVGVVGKFVEF FGPGVAQLSIADRATIANMCPEYGATAAFFPVDEVSITYLVQTGRDEEKLKYIKKYLQAVGMFRDFNDPS QDPDFTQVVELDLKTVVPCCSGPKRPQDKVAVSDMKKDFESCLGAKQGFKGFQVAPEHHNDHKTFIYDNT EFTLAHGSVVIAAITSCTNTSNPSVMLGAGLLAKKAVDAGLNVMPYIKTSLSPGSGVVTYYLQESGVMPY LSQLGFDVVGYGCMTCIGNSGPLPEPVVEAITQGDLVAVGVLSGNRNFEGRVHPNTRANYLASPPLVIAY AIAGTIRIDFEKEPLGVNAKGQQVFLKDIWPTRDEIQAVERQYVIPGMFKEVYQKIETVNESWNALATPS DKLFFWNSKSTYIKSPPFFENLTLDLQPPKSIVDAYVLLNLGDSVTTDHISPAGNIARNSPAARYLTNRG LTPREFNSYGSRRGNDAVMARGTFANIRLLNRFLNKQAPQTIHLPSGEILDVFDAAERYQQAGLPLIVLA GKEYGAGSSRDWAAKGPFLLGIKAVLAESYERIHRSNLVGMGVIPLEYLPGENADALGLTGQERYTIIIP ENLKPQMKVQVKLDTGKTFQAVMRFDTDVELTYFLNGGILNYMIRKMAK TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_002197 |
| ORF Size | 2667 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_002197.3 |
| RefSeq Size | 3498 bp |
| RefSeq ORF | 2670 bp |
| Locus ID | 48 |
| UniProt ID | P21399 |
| Domains | Aconitase_C, aconitase |
| Protein Families | Druggable Genome |
| Protein Pathways | Citrate cycle (TCA cycle), Glyoxylate and dicarboxylate metabolism, Metabolic pathways |
| Gene Summary | The protein encoded by this gene is a bifunctional, cytosolic protein that functions as an essential enzyme in the TCA cycle and interacts with mRNA to control the levels of iron inside cells. When cellular iron levels are high, this protein binds to a 4Fe-4S cluster and functions as an aconitase. Aconitases are iron-sulfur proteins that function to catalyze the conversion of citrate to isocitrate. When cellular iron levels are low, the protein binds to iron-responsive elements (IREs), which are stem-loop structures found in the 5' UTR of ferritin mRNA, and in the 3' UTR of transferrin receptor mRNA. When the protein binds to IRE, it results in repression of translation of ferritin mRNA, and inhibition of degradation of the otherwise rapidly degraded transferrin receptor mRNA. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. Alternative splicing results in multiple transcript variants [provided by RefSeq, Jan 2014] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC201857 | ACO1 (Myc-DDK-tagged)-Human aconitase 1, soluble (ACO1) |
CNY 6,512.00 |
|
| RC201857L1 | Lenti ORF clone of Human aconitase 1, soluble (ACO1), Myc-DDK-tagged |
CNY 8,912.00 |
|
| RC201857L2 | Lenti ORF clone of Human aconitase 1, soluble (ACO1), mGFP tagged |
CNY 9,030.00 |
|
| RC201857L3 | Lenti ORF clone of Human aconitase 1, soluble (ACO1), Myc-DDK-tagged |
CNY 8,912.00 |
|
| RC201857L4 | Lenti ORF clone of Human aconitase 1, soluble (ACO1), mGFP tagged |
CNY 8,912.00 |
|
| SC126974 | ACO1 (untagged)-Human aconitase 1, soluble (ACO1) |
CNY 6,520.00 |
|
| SC322429 | ACO1 (untagged)-Human aconitase 1, soluble (ACO1) |
CNY 6,520.00 |
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