GOT2 (NM_002080) Human Tagged ORF Clone
CAT#: RG201826
- TrueORF®
GOT2 (tGFP-tagged) - Human glutamic-oxaloacetic transaminase 2, mitochondrial (aspartate aminotransferase 2) (GOT2), nuclear gene encoding mitochondrial protein
ORF Plasmid: DDK
"NM_002080" in other vectors (5)
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CNY 7,088.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | TurboGFP |
Synonyms | DEE82; KAT4; KATIV; KYAT4; mitAAT |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RG201826 representing NM_002080
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCCTGCTGCACTCCGGCCGCGTCCTCCCCGGGATCGCCGCCGCCTTCCACCCGGGCCTCGCCGCCG CGGCCTCTGCCAGAGCCAGCTCCTGGTGGACCCATGTGGAAATGGGACCTCCAGATCCCATTCTGGGAGT CACTGAAGCCTTTAAGAGGGACACCAATAGCAAAAAGATGAATCTGGGAGTTGGTGCCTACCGGGATGAT AATGGAAAGCCTTACGTTCTGCCTAGCGTCCGCAAGGCAGAGGCCCAGATTGCCGCAAAAAATTTGGACA AGGAATACCTGCCCATTGGGGGACTGGCTGAATTTTGCAAGGCATCTGCAGAACTAGCCCTGGGTGAGAA CAGCGAAGTCTTGAAGAGTGGCCGGTTTGTCACTGTGCAGACCATTTCTGGAACTGGAGCCTTAAGGATC GGAGCCAGTTTTCTGCAAAGATTTTTTAAGTTCAGCCGAGATGTCTTTCTGCCCAAACCAACCTGGGGAA ACCACACACCCATCTTCAGGGATGCTGGCATGCAGCTACAAGGTTATCGGTATTATGACCCCAAGACTTG CGGTTTTGACTTCACAGGCGCTGTGGAGGATATTTCAAAAATACCAGAGCAGAGTGTTCTTCTTCTGCAT GCCTGCGCCCACAATCCCACGGGAGTGGACCCGCGTCCGGAACAGTGGAAGGAAATAGCAACAGTGGTGA AGAAAAGGAATCTCTTTGCGTTCTTTGACATGGCCTACCAAGGCTTTGCCAGTGGTGATGGTGATAAGGA TGCCTGGGCTGTGCGCCACTTCATCGAACAGGGCATTAATGTTTGCCTCTGCCAATCATATGCCAAGAAC ATGGGCTTATATGGTGAGCGTGTAGGAGCCTTCACTATGGTCTGCAAAGATGCGGATGAAGCCAAAAGGG TAGAGTCACAGTTGAAGATCTTGATCCGTCCCATGTATTCCAACCCTCCCCTCAATGGGGCCCGGATTGC TGCTGCCATTCTGAACACCCCAGATTTGCGAAAACAATGGCTGCAAGAAGTGAAAGTCATGGCTGACCGC ATCATTGGCATGCGGACTCAACTGGTCTCCAACCTCAAGAAGGAGGGTTCCACCCACAATTGGCAACACA TCACCGACCAAATTGGCATGTTCTGTTTCACAGGGCTAAAGCCTGAACAGGTGGAGCGGCTGATCAAGGA GTTCTCCATCTACATGACAAAAGATGGCCGCATCTCTGTGGCAGGGGTCACCTCCAGCAACGTGGGCTAC CTTGCCCATGCCATTCACCAGGCCACCAAG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG201826 representing NM_002080
Red=Cloning site Green=Tags(s) MALLHSGRVLPGIAAAFHPGLAAAASARASSWWTHVEMGPPDPILGVTEAFKRDTNSKKMNLGVGAYRDD NGKPYVLPSVRKAEAQIAAKNLDKEYLPIGGLAEFCKASAELALGENSEVLKSGRFVTVQTISGTGALRI GASFLQRFFKFSRDVFLPKPTWGNHTPIFRDAGMQLQGYRYYDPKTCGFDFTGAVEDISKIPEQSVLLLH ACAHNPTGVDPRPEQWKEIATVVKKRNLFAFFDMAYQGFASGDGDKDAWAVRHFIEQGINVCLCQSYAKN MGLYGERVGAFTMVCKDADEAKRVESQLKILIRPMYSNPPLNGARIAAAILNTPDLRKQWLQEVKVMADR IIGMRTQLVSNLKKEGSTHNWQHITDQIGMFCFTGLKPEQVERLIKEFSIYMTKDGRISVAGVTSSNVGY LAHAIHQATK TRTRPLE - GFP Tag - V |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_002080 |
ORF Size | 1290 bp |
OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_002080.2, NP_002071.2 |
RefSeq Size | 2488 bp |
RefSeq ORF | 1293 bp |
Locus ID | 2806 |
UniProt ID | P00505 |
Domains | aminotran_1_2 |
Protein Families | Stem cell - Pluripotency |
Protein Pathways | Alanine, aspartate and glutamate metabolism, Arginine and proline metabolism, Cysteine and methionine metabolism, Metabolic pathways, Phenylalanine, tyrosine and tryptophan biosynthesis, Phenylalanine metabolism, Tyrosine metabolism |
Gene Summary | Glutamic-oxaloacetic transaminase is a pyridoxal phosphate-dependent enzyme which exists in cytoplasmic and inner-membrane mitochondrial forms, GOT1 and GOT2, respectively. GOT plays a role in amino acid metabolism and the urea and tricarboxylic acid cycles. The two enzymes are homodimeric and show close homology. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2013] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC201826 | GOT2 (Myc-DDK-tagged)-Human glutamic-oxaloacetic transaminase 2, mitochondrial (aspartate aminotransferase 2) (GOT2), nuclear gene encoding mitochondrial protein |
CNY 5,488.00 |
|
RC201826L3 | Lenti-ORF clone of GOT2 (Myc-DDK-tagged)-Human glutamic-oxaloacetic transaminase 2, mitochondrial (aspartate aminotransferase 2) (GOT2), nuclear gene encoding mitochondrial protein |
CNY 5,890.00 |
|
RC201826L4 | Lenti-ORF clone of GOT2 (mGFP-tagged)-Human glutamic-oxaloacetic transaminase 2, mitochondrial (aspartate aminotransferase 2) (GOT2), nuclear gene encoding mitochondrial protein |
CNY 5,890.00 |
|
SC127269 | GOT2 (untagged)-Human glutamic-oxaloacetic transaminase 2, mitochondrial (aspartate aminotransferase 2) (GOT2), nuclear gene encoding mitochondrial protein |
CNY 5,488.00 |
|
SC320593 | GOT2 (untagged)-Human glutamic-oxaloacetic transaminase 2, mitochondrial (aspartate aminotransferase 2) (GOT2), nuclear gene encoding mitochondrial protein |
CNY 5,488.00 |