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Nucleobindin 1 (NUCB1) (NM_006184) Human Tagged ORF Clone
CAT＃: RG201786
- TrueORF®
NUCB1 (tGFP-tagged) - Human nucleobindin 1 (NUCB1)
ORF Plasmid: DDK
"NM_006184" in other vectors (6)
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CNY 5,256.00
| Cited in 1 publication. |
Product images
CNY 1,999.00
CNY 2,700.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | CALNUC; NUC |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG201786 representing NM_006184
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCTCCCTCTGGGCCCCGAGGAACCCTCCTTCTGTTGCCGCTGCTGCTGCTGCTCCTGCTTCGCGCCG TGCTGGCTGTCCCCCTGGAGCGAGGGGCGCCCAACAAGGAGGAGACCCCTGCGACTGAGAGTCCCGACAC AGGCCTGTACTACCACCGGTACCTCCAGGAGGTCATCGATGTACTGGAGACGGATGGGCATTTCCGAGAG AAGCTGCAGGCTGCCAATGCGGAGGACATCAAGAGCGGGAAGCTGAGCCGAGAGCTGGACTTTGTCAGCC ACCACGTCCGCACCAAGCTGGATGAGCTCAAGCGACAGGAGGTGTCACGGCTGCGGATGCTGCTCAAGGC CAAGATGGACGCCGAGCAGGATCCCAATGTACAGGTGGATCATCTGAATCTCCTGAAACAGTTTGAACAC CTGGACCCTCAGAACCAGCATACATTCGAGGCCCGCGACCTGGAGCTGCTGATCCAGACGGCCACCCGGG ACCTTGCCCAGTACGACGCAGCCCATCATGAAGAGTTCAAGCGCTACGAGATGCTTAAGGAACACGAGAG ACGGCGTTATCTGGAGTCACTGGGAGAGGAGCAGAGAAAGGAGGCGGAGAGGAAGCTGGAAGAGCAACAG CGCCGGCACCGCGAGCACCCTAAAGTCAACGTGCCTGGCAGCCAAGCCCAGTTGAAGGAGGTGTGGGAGG AGCTGGATGGACTGGACCCCAACAGGTTTAACCCCAAGACCTTCTTCATACTGCATGATATCAACAGTGA TGGTGTCCTGGATGAGCAGGAGCTGGAGGCACTCTTCACCAAGGAGCTGGAGAAAGTGTACGACCCAAAG AATGAGGAGGACGACATGCGGGAGATGGAGGAGGAGCGACTGCGCATGCGGGAGCATGTGATGAAGAATG TGGACACCAACCAGGACCGCCTCGTGACCCTGGAGGAGTTCCTCGCATCCACTCAGAGGAAGGAGTTTGG GGACACCGGGGAGGGCTGGGAGACAGTGGAGATGCACCCTGCCTACACCGAGGAAGAGCTGAGGCGCTTT GAAGAGGAGCTGGCTGCCCGGGAGGCAGAGCTGAATGCCAAGGCCCAGCGCCTCAGCCAGGAGACAGAGG CTCTAGGGCGGTCCCAGGGCCGCCTGGAGGCCCAGAAGAGAGAGCTGCAGCAGGCTGTGCTGCACATGGA GCAGCGGAAGCAGCAGCAGCAGCAGCAGCAAGGCCACAAGGCCCCGGCTGCCCACCCTGAGGGGCAGCTC AAGTTCCACCCAGACACAGACGATGTACCTGTCCCAGCTCCAGCCGGTGACCAGAAGGAGGTGGACACTT CAGAAAAGAAACTTCTCGAGCGGCTCCCTGAGGTTGAGGTGCCCCAGCATCTG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG201786 representing NM_006184
Red=Cloning site Green=Tags(s) MPPSGPRGTLLLLPLLLLLLLRAVLAVPLERGAPNKEETPATESPDTGLYYHRYLQEVIDVLETDGHFRE KLQAANAEDIKSGKLSRELDFVSHHVRTKLDELKRQEVSRLRMLLKAKMDAEQDPNVQVDHLNLLKQFEH LDPQNQHTFEARDLELLIQTATRDLAQYDAAHHEEFKRYEMLKEHERRRYLESLGEEQRKEAERKLEEQQ RRHREHPKVNVPGSQAQLKEVWEELDGLDPNRFNPKTFFILHDINSDGVLDEQELEALFTKELEKVYDPK NEEDDMREMEEERLRMREHVMKNVDTNQDRLVTLEEFLASTQRKEFGDTGEGWETVEMHPAYTEEELRRF EEELAAREAELNAKAQRLSQETEALGRSQGRLEAQKRELQQAVLHMEQRKQQQQQQQGHKAPAAHPEGQL KFHPDTDDVPVPAPAGDQKEVDTSEKKLLERLPEVEVPQHL TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_006184 |
| ORF Size | 1383 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_006184.6 |
| RefSeq Size | 2302 bp |
| RefSeq ORF | 1386 bp |
| Locus ID | 4924 |
| UniProt ID | Q02818 |
| Domains | EFh |
| Gene Summary | This gene encodes a member of a small calcium-binding EF-hand protein family. The encoded protein is thought to have a key role in Golgi calcium homeostasis and Ca(2+)-regulated signal transduction events. [provided by RefSeq, Jun 2010] |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
Nucleobindin-1 regulates ECM degradation by promoting intra-Golgi trafficking of MMPs
,null,
The Journal of Cell Biology
,PubMed ID 32479594
[NUCB1]
|
Documents
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| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC201786 | NUCB1 (Myc-DDK-tagged)-Human nucleobindin 1 (NUCB1) |
CNY 3,656.00 |
|
| RC201786L1 | Lenti ORF clone of Human nucleobindin 1 (NUCB1), Myc-DDK-tagged |
CNY 6,056.00 |
|
| RC201786L2 | Lenti ORF clone of Human nucleobindin 1 (NUCB1), mGFP tagged |
CNY 5,890.00 |
|
| RC201786L3 | Lenti ORF clone of Human nucleobindin 1 (NUCB1), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC201786L4 | Lenti ORF clone of Human nucleobindin 1 (NUCB1), mGFP tagged |
CNY 5,890.00 |
|
| SC320256 | NUCB1 (untagged)-Human nucleobindin 1 (NUCB1) |
CNY 3,656.00 |
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