United States
Germany
Japan
United Kingdom
China
Histone H1x (H1FX) (NM_006026) Human Tagged ORF Clone
CAT＃: RG201735
- TrueORF®
H1FX (tGFP-tagged) - Human H1 histone family, member X (H1FX)
ORF Plasmid: DDK
"NM_006026" in other vectors (4)
Need custom modification / cloning service?
Get a free quote
CNY 4,370.00
Product images
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | H1.10; H1FX; H1X |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG201735 representing NM_006026
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCCGTGGAGCTCGAGGAGGCCCTGCCAGTGACGACCGCCGAGGGAATGGCCAAGAAGGTGACCAAGG CTGGCGGCTCGGCGGCGTTGTCCCCATCTAAGAAGAGGAAGAATAGCAAGAAGAAGAACCAGCCGGGCAA GTACAGCCAGCTGGTGGTGGAGACCATCCGTAGGCTGGGCGAGCGCAACGGCTCGTCGCTGGCCAAGATC TACACCGAGGCCAAGAAGGTTCCGTGGTTCGACCAGCAGAATGGGCGCACCTACCTCAAGTACTCGATCA AGGCGCTGGTGCAGAACGACACGCTTCTGCAGGTGAAGGGCACCGGCGCCAACGGTTCCTTCAAGCTCAA CCGCAAGAAGCTGGAGGGCGGCGGGGAGCGGCGCGGAGCCCCGGCGGCCGCCACCGCCCCGGCCCCCACC GCGCACAAAGCGAAGAAGGCAGCCCCGGGCGCGGCCGGCTCCCGGCGCGCGGACAAGAAGCCCGCCAGGG GCCAGAAGCCGGAGCAGCGCTCGCACAAGAAGGGCGCTGGCGCCAAGAAGGACAAAGGCGGCAAGGCCAA GAAGACGGCGGCCGCCGGGGGCAAGAAGGTGAAGAAGGCGGCCAAGCCCAGCGTCCCCAAAGTGCCCAAG GGCCGCAAG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG201735 representing NM_006026
Red=Cloning site Green=Tags(s) MSVELEEALPVTTAEGMAKKVTKAGGSAALSPSKKRKNSKKKNQPGKYSQLVVETIRRLGERNGSSLAKI YTEAKKVPWFDQQNGRTYLKYSIKALVQNDTLLQVKGTGANGSFKLNRKKLEGGGERRGAPAAATAPAPT AHKAKKAAPGAAGSRRADKKPARGQKPEQRSHKKGAGAKKDKGGKAKKTAAAGGKKVKKAAKPSVPKVPK GRK TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_006026 |
| ORF Size | 639 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_006026.4 |
| RefSeq Size | 1503 bp |
| RefSeq ORF | 642 bp |
| Locus ID | 8971 |
| UniProt ID | Q92522 |
| Domains | linker_histone |
| Protein Families | ES Cell Differentiation/IPS |
| Gene Summary | Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene encodes a replication-independent histone that is a member of the histone H1 family. [provided by RefSeq, Oct 2015] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC201735 | H1FX (Myc-DDK-tagged)-Human H1 histone family, member X (H1FX) |
CNY 2,400.00 |
|
| RC201735L3 | Lenti ORF clone of Human H1 histone family, member X (H1FX), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC201735L4 | Lenti ORF clone of Human H1 histone family, member X (H1FX), mGFP tagged |
CNY 5,890.00 |
|
| SC320231 | H1FX (untagged)-Human H1 histone family, member X (H1FX) |
CNY 2,400.00 |
其它Histone H1x产品
