PWP1 (NM_007062) Human Tagged ORF Clone

CAT#: RG200892

  • TrueORF®

PWP1 (tGFP-tagged) - Human PWP1 homolog (S. cerevisiae) (PWP1)

ORF Plasmid: DDK tGFP



  "NM_007062" in other vectors (5)

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CNY 5,040.00


货期*
4周

规格
    • 10 ug

Product images

经常一起买 (5)
pCMV6-AC-GFP, mammalian vector with C-terminal tGFP tag, 10ug
    • 10 ug

CNY 6,080.00


Mouse monoclonal turboGFP antibody, clone OTI2H8
    • 100 ul

CNY 1,000.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


PWP1 rabbit polyclonal antibody
    • 100 ul

CNY 1,999.00
CNY 3,280.00

Specifications

Product Data
Type Human Tagged ORF Clone
Tag TurboGFP
Synonyms IEF-SSP-9502
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RG200892 representing NM_007062
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAACCGCAGCCGCCAGGTGACGTGCGTGGCCTGGGTCCGCTGCGGCGTGGCCAAAGAGACACCAGACA
AGGTAGAGCTGAGTAAAGAAGAAGTAAAACGCCTCATTGCTGAGGCAAAGGAGAAATTGCAAGAAGAAGG
TGGTGGCAGTGATGAAGAGGAGACAGGCAGTCCTTCAGAAGATGGCATGCAGAGTGCACGCACCCAGGCA
CGCCCAAGAGAGCCCCTGGAGGATGGTGACCCAGAGGATGACAGGACGCTTGATGATGATGAGCTGGCTG
AGTACGACTTAGATAAATATGATGAGGAAGGTGACCCAGATGCTGAGACTCTTGGTGAATCTCTCTTGGG
TCTTACGGTCTACGGGAGTAATGATCAAGATCCTTACGTTACTCTGAAAGATACAGAACAATATGAACGT
GAAGATTTCTTGATTAAGCCCAGTGATAATCTTATAGTTTGTGGCCGAGCTGAACAGGACCAGTGCAATT
TAGACGTGCATGTTTATAATCAAGAAGAAGACTCTTTTTATGTACACCATGATATACTCTTGTCTGCATA
TCCTCTGAGTGTGGAATGGCTGAATTTTGATCCTAGCCCAGATGATTCTACTGGAAATTACATTGCTGTA
GGAAACATGACCCCTGTTATTGAAGTGTGGGACCTTGATATAGTGGACTCTTTAGAGCCAGTCTTCACAC
TCGGAAGTAAACTTTCAAAAAAGAAGAAAAAGAAAGGAAAGAAGAGTTCCTCAGCAGAAGGGCATACCGA
TGCTGTCCTTGACCTTTCATGGAATAAGCTAATCAGAAATGTTTTAGCAAGTGCATCAGCTGACAACACT
GTAATTCTGTGGGATATGTCCTTGGGGAAACCAGCAGCTAGCCTCGCTGTACACACAGACAAGGTCCAAA
CACTGCAGTTTCATCCATTTGAAGCACAGACTCTGATTTCTGGCTCATATGATAAGTCAGTGGCTTTGTA
TGACTGCCGAAGTCCAGATGAAAGCCATCGAATGTGGCGATTCAGTGGGCAGATAGAGAGAGTGACTTGG
AATCACTTTTCACCTTGTCATTTCTTGGCCAGTACAGATGACGGCTTTGTATATAATTTGGATGCACGTT
CAGATAAGCCAATTTTTACACTTAATGCACACAATGATGAAATCTCTGGTCTTGATCTTAGCAGTCAAAT
CAAGGGCTGTCTCGTGACTGCTTCAGCTGACAAATACGTGAAGATCTGGGACATCTTAGGAGATAGGCCA
AGTCTAGTTCATTCTAGGGACATGAAAATGGGAGTTCTCTTCTGTTCTTCATGTTGCCCTGATTTGCCAT
TTATTTATGCCCTTCGAGGTCAAAAAGAAGGGCTTCGGGTCTGGGATATAAGCACAGTCTCTTCAGTAAA
TGAAGCATTTGGAAGACGAGAGAGGCTTGTTCTTGGGAGTGCAAGAAATTCATCTATTAGTGGCCCTTTT
GGCAGCAGGAGCTCAGATACACCCATGGAGTCT


ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
>RG200892 representing NM_007062
Red=Cloning site Green=Tags(s)

MNRSRQVTCVAWVRCGVAKETPDKVELSKEEVKRLIAEAKEKLQEEGGGSDEEETGSPSEDGMQSARTQA
RPREPLEDGDPEDDRTLDDDELAEYDLDKYDEEGDPDAETLGESLLGLTVYGSNDQDPYVTLKDTEQYER
EDFLIKPSDNLIVCGRAEQDQCNLDVHVYNQEEDSFYVHHDILLSAYPLSVEWLNFDPSPDDSTGNYIAV
GNMTPVIEVWDLDIVDSLEPVFTLGSKLSKKKKKKGKKSSSAEGHTDAVLDLSWNKLIRNVLASASADNT
VILWDMSLGKPAASLAVHTDKVQTLQFHPFEAQTLISGSYDKSVALYDCRSPDESHRMWRFSGQIERVTW
NHFSPCHFLASTDDGFVYNLDARSDKPIFTLNAHNDEISGLDLSSQIKGCLVTASADKYVKIWDILGDRP
SLVHSRDMKMGVLFCSSCCPDLPFIYALRGQKEGLRVWDISTVSSVNEAFGRRERLVLGSARNSSISGPF
GSRSSDTPMES

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_007062
ORF Size 1503 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_007062.1, NP_008993.1
RefSeq Size 1853 bp
RefSeq ORF 1506 bp
Locus ID 11137
UniProt ID Q13610
Domains WD40
Protein Families Transcription Factors
Gene Summary The protein encoded by this gene contains several WD-40 repeats and is found mostly in the nucleus. The expression and localization of this protein are cell cycle dependent. Expression of this gene is upregulated in pancreatic adenocarcinoma. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Dec 2015]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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