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HAND1 (NM_004821) Human Tagged ORF Clone
CAT＃: RG200513
- TrueORF®
HAND1 (tGFP-tagged) - Human heart and neural crest derivatives expressed 1 (HAND1)
ORF Plasmid: DDK
"NM_004821" in other vectors (6)
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CNY 4,000.00
CNY 4,370.00
Product images
CNY 1,999.00
CNY 2,700.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | bHLHa27; eHand; Hxt; Thing1 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG200513 representing NM_004821
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAACCTCGTGGGCAGCTACGCACACCATCACCACCATCACCACCCGCACCCTGCGCACCCCATGCTCC ACGAACCCTTCCTCTTCGGTCCGGCCTCGCGCTGTCATCAGGAAAGGCCCTACTTCCAGAGCTGGCTGCT GAGCCCGGCTGACGCTGCCCCGGACTTCCCTGCGGGCGGGCCGCCGCCCGCGGCCGCTGCAGCCGCCACC GCCTATGGTCCTGACGCCAGGCCTGGGCAGAGCCCCGGGCGGCTGGAGGCGCTTGGCGGCCGTCTTGGCC GGCGGAAAGGCTCAGGACCCAAGAAGGAGCGGAGACGCACTGAGAGCATTAACAGCGCATTCGCGGAGTT GCGCGAGTGCATCCCCAACGTGCCGGCCGACACCAAGCTCTCCAAGATCAAGACTCTGCGCCTAGCCACC AGCTACATCGCCTACCTGATGGACGTGCTGGCCAAGGATGCACAGTCTGGCGATCCCGAGGCCTTCAAGG CTGAACTCAAGAAGGCGGATGGCGGCCGTGAGAGCAAGCGGAAAAGGGAGCTGCAGCAGCACGAAGGTTT TCCTCCTGCCCTGGGCCCAGTCGAGAAGAGGATTAAAGGACGCACCGGCTGGCCGCAGCAAGTCTGGGCG CTGGAGTTAAACCAG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG200513 representing NM_004821
Red=Cloning site Green=Tags(s) MNLVGSYAHHHHHHHPHPAHPMLHEPFLFGPASRCHQERPYFQSWLLSPADAAPDFPAGGPPPAAAAAAT AYGPDARPGQSPGRLEALGGRLGRRKGSGPKKERRRTESINSAFAELRECIPNVPADTKLSKIKTLRLAT SYIAYLMDVLAKDAQSGDPEAFKAELKKADGGRESKRKRELQQHEGFPPALGPVEKRIKGRTGWPQQVWA LELNQ TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_004821 |
| ORF Size | 645 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_004821.3 |
| RefSeq Size | 1750 bp |
| RefSeq ORF | 648 bp |
| Locus ID | 9421 |
| UniProt ID | O96004 |
| Protein Families | Druggable Genome, ES Cell Differentiation/IPS, Transcription Factors |
| Gene Summary | The protein encoded by this gene belongs to the basic helix-loop-helix family of transcription factors. This gene product is one of two closely related family members, the HAND proteins, which are asymmetrically expressed in the developing ventricular chambers and play an essential role in cardiac morphogenesis. Working in a complementary fashion, they function in the formation of the right ventricle and aortic arch arteries, implicating them as mediators of congenital heart disease. In addition, it has been suggested that this transcription factor may be required for early trophoblast differentiation. [provided by RefSeq, Jul 2008] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC200513 | HAND1 (Myc-DDK-tagged)-Human heart and neural crest derivatives expressed 1 (HAND1) |
CNY 2,400.00 |
|
| RC200513L1 | Lenti ORF clone of Human heart and neural crest derivatives expressed 1 (HAND1), Myc-DDK-tagged |
CNY 4,800.00 |
|
| RC200513L2 | Lenti ORF clone of Human heart and neural crest derivatives expressed 1 (HAND1), mGFP tagged |
CNY 4,800.00 |
|
| RC200513L3 | Lenti ORF clone of Human heart and neural crest derivatives expressed 1 (HAND1), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC200513L4 | Lenti ORF clone of Human heart and neural crest derivatives expressed 1 (HAND1), mGFP tagged |
CNY 5,890.00 |
|
| SC122690 | HAND1 (untagged)-Human heart and neural crest derivatives expressed 1 (HAND1) |
CNY 2,400.00 |
