FHL2 (NM_201557) Human Tagged ORF Clone
CAT#: RG200398
- TrueORF®
FHL2 (tGFP-tagged) - Human four and a half LIM domains 2 (FHL2), transcript variant 4
ORF Plasmid: DDK
"NM_201557" in other vectors (4)
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CNY 4,370.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | TurboGFP |
Synonyms | AAG11; DRAL; FHL-2; SLIM-3; SLIM3 |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RG200398 representing NM_201557
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGACTGAGCGCTTTGACTGCCACCATTGCAACGAATCTCTCTTTGGCAAGAAGTACATCCTGCGGGAGG AGAGCCCCTACTGCGTGGTGTGCTTTGAGACCCTGTTCGCCAACACCTGCGAGGAGTGTGGGAAGCCCAT CGGCTGTGACTGCAAGGACTTGTCTTACAAGGACCGGCACTGGCATGAAGCCTGTTTCCACTGCTCGCAG TGCAGAAACTCACTGGTGGACAAGCCCTTTGCTGCCAAGGAGGACCAGCTGCTCTGTACAGACTGCTATT CCAACGAGTACTCATCCAAGTGCCAGGAATGCAAGAAGACCATCATGCCAGGTACCCGCAAGATGGAGTA CAAGGGCAGCAGCTGGCATGAGACCTGCTTCATCTGCCACCGCTGCCAGCAGCCAATTGGAACCAAGAGT TTCATCCCCAAAGACAATCAGAATTTCTGTGTGCCCTGCTATGAGAAACAACATGCCATGCAGTGCGTTC AGTGCAAAAAGCCCATCACCACGGGAGGGGTCACTTACCGGGAGCAGCCCTGGCACAAGGAGTGCTTCGT GTGCACCGCCTGCAGGAAGCAGCTGTCTGGGCAGCGCTTCACAGCTCGCGATGACTTTGCCTACTGCCTG AACTGCTTCTGTGACTTGTATGCCAAGAAGTGTGCTGGGTGCACCAACCCCATCAGCGGACTTGGTGGCA CAAAATACATCTCCTTTGAGGAACGGCAGTGGCATAACGACTGCTTTAACTGTAAGAAGTGCTCCCTCTC ACTGGTGGGGCGTGGCTTCCTCACAGAGAGGGACGACATCCTGTGCCCCGACTGTGGGAAAGACATC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG200398 representing NM_201557
Red=Cloning site Green=Tags(s) MTERFDCHHCNESLFGKKYILREESPYCVVCFETLFANTCEECGKPIGCDCKDLSYKDRHWHEACFHCSQ CRNSLVDKPFAAKEDQLLCTDCYSNEYSSKCQECKKTIMPGTRKMEYKGSSWHETCFICHRCQQPIGTKS FIPKDNQNFCVPCYEKQHAMQCVQCKKPITTGGVTYREQPWHKECFVCTACRKQLSGQRFTARDDFAYCL NCFCDLYAKKCAGCTNPISGLGGTKYISFEERQWHNDCFNCKKCSLSLVGRGFLTERDDILCPDCGKDI TRTRPLE - GFP Tag - V |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_201557 |
ORF Size | 837 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_201557.4 |
RefSeq Size | 1424 bp |
RefSeq ORF | 840 bp |
Locus ID | 2274 |
UniProt ID | Q14192 |
Protein Families | Druggable Genome |
Gene Summary | This gene encodes a member of the four-and-a-half-LIM-only protein family. Family members contain two highly conserved, tandemly arranged, zinc finger domains with four highly conserved cysteines binding a zinc atom in each zinc finger. This protein is thought to have a role in the assembly of extracellular membranes. Also, this gene is down-regulated during transformation of normal myoblasts to rhabdomyosarcoma cells and the encoded protein may function as a link between presenilin-2 and an intracellular signaling pathway. Multiple alternatively spliced variants encoding different isoforms have been identified. [provided by RefSeq, Jan 2016] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC200398 | FHL2 (Myc-DDK-tagged)-Human four and a half LIM domains 2 (FHL2), transcript variant 4 |
CNY 2,400.00 |
|
RC200398L3 | Lenti ORF clone of Human four and a half LIM domains 2 (FHL2), transcript variant 4, Myc-DDK-tagged |
CNY 5,890.00 |
|
RC200398L4 | Lenti ORF clone of Human four and a half LIM domains 2 (FHL2), transcript variant 4, mGFP tagged |
CNY 5,890.00 |
|
SC317400 | FHL2 (untagged)-Human four and a half LIM domains 2 (FHL2), transcript variant 4 |
CNY 6,270.00 |