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BLMH (NM_000386) Human Tagged ORF Clone
CAT＃: RG200393
- TrueORF®
BLMH (tGFP-tagged) - Human bleomycin hydrolase (BLMH)
ORF Plasmid: DDK
"NM_000386" in other vectors (6)
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CNY 5,256.00
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热销推荐Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | BH; BMH |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG200393 representing NM_000386
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGCAGCTCGGGACTGAATTCGGAGAAGGTAGCTGCTCTGATACAGAAACTGAATTCCGACCCCCAGT TCGTACTTGCCCAGAATGTCGGGACCACCCACGACCTGCTGGACATCTGTCTGAAGCGGGCCACGGTGCA GCGCGCGCAGCATGTGTTCCAGCACGCCGTGCCCCAGGAGGGCAAGCCAATCACCAACCAGAAGAGCTCA GGGCGATGCTGGATCTTTTCTTGTCTGAATGTTATGAGGCTTCCATTCATGAAAAAGTTAAATATTGAAG AATTTGAGTTTAGCCAATCTTACCTGTTTTTTTGGGACAAGGTTGAACGCTGTTATTTCTTCTTGAGTGC TTTTGTGGACACAGCCCAGAGAAAGGAGCCTGAGGATGGGAGGCTGGTGCAGTTTTTGCTTATGAACCCT GCAAATGATGGTGGCCAATGGGATATGCTTGTTAATATTGTTGAAAAATATGGTGTTATCCCTAAGAAAT GCTTCCCTGAATCTTATACAACAGAGGCAACCAGAAGGATGAATGATATTCTGAATCACAAGATGAGAGA ATTCTGTATACGACTGCGGAACCTGGTACACAGTGGAGCAACCAAAGGAGAAATCTCGGCCACACAGGAC GTCATGATGGAGGAGATATTCCGAGTGGTGTGCATCTGTTTGGGTAATCCACCAGAGACATTCACCTGGG AATATCGAGACAAAGATAAAAATTATCAGAAAATTGGCCCCATAACACCCTTGGAGTTTTACAGGGAACA TGTCAAGCCACTCTTCAATATGGAAGATAAGATTTGTTTAGTGAATGACCCTAGGCCCCAGCACAAGTAC AACAAACTTTACACAGTGGAATACTTAAGCAATATGGTTGGAGGGAGAAAAACTCTATACAACAACCAGC CCATTGACTTCCTGAAAAAGATGGTTGCTGCCTCCATCAAAGATGGAGAGGCTGTGTGGTTTGGCTGTGA TGTTGGAAAACACTTCAATAGCAAGCTGGGCCTCAGTGACATGAATCTCTATGACCATGAGTTAGTGTTT GGTGTCTCCTTGAAGAACATGAATAAAGCGGAGAGGCTGACTTTTGGTGAGTCACTTATGACCCACGCCA TGACCTTCACTGCTGTCTCAGAGAAGGATGATCAGGATGGTGCTTTCACAAAATGGAGAGTGGAGAATTC ATGGGGTGAAGACCATGGCCACAAAGGTTACCTGTGCATGACAGATGAGTGGTTCTCTGAGTATGTCTAC GAAGTGGTGGTGGACAGGAAGCATGTCCCTGAAGAGGTGCTAGCTGTGTTAGAGCAGGAACCCATTATCC TGCCAGCATGGGACCCCATGGGAGCTTTGGCTGAG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG200393 representing NM_000386
Red=Cloning site Green=Tags(s) MSSSGLNSEKVAALIQKLNSDPQFVLAQNVGTTHDLLDICLKRATVQRAQHVFQHAVPQEGKPITNQKSS GRCWIFSCLNVMRLPFMKKLNIEEFEFSQSYLFFWDKVERCYFFLSAFVDTAQRKEPEDGRLVQFLLMNP ANDGGQWDMLVNIVEKYGVIPKKCFPESYTTEATRRMNDILNHKMREFCIRLRNLVHSGATKGEISATQD VMMEEIFRVVCICLGNPPETFTWEYRDKDKNYQKIGPITPLEFYREHVKPLFNMEDKICLVNDPRPQHKY NKLYTVEYLSNMVGGRKTLYNNQPIDFLKKMVAASIKDGEAVWFGCDVGKHFNSKLGLSDMNLYDHELVF GVSLKNMNKAERLTFGESLMTHAMTFTAVSEKDDQDGAFTKWRVENSWGEDHGHKGYLCMTDEWFSEYVY EVVVDRKHVPEEVLAVLEQEPIILPAWDPMGALAE TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_000386 |
| ORF Size | 1365 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_000386.4 |
| RefSeq Size | 2417 bp |
| RefSeq ORF | 1368 bp |
| Locus ID | 642 |
| UniProt ID | Q13867 |
| Domains | Pept_C1-like |
| Protein Families | Druggable Genome, Protease |
| Gene Summary | Bleomycin hydrolase (BMH) is a cytoplasmic cysteine peptidase that is highly conserved through evolution; however, the only known activity of the enzyme is metabolic inactivation of the glycopeptide bleomycin (BLM), an essential component of combination chemotherapy regimens for cancer. The protein contains the signature active site residues of the cysteine protease papain superfamily. [provided by RefSeq, Jul 2008] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC200393 | BLMH (Myc-DDK-tagged)-Human bleomycin hydrolase (BLMH) |
CNY 3,656.00 |
|
| RC200393L1 | Lenti ORF clone of Human bleomycin hydrolase (BLMH), Myc-DDK-tagged |
CNY 6,056.00 |
|
| RC200393L2 | Lenti ORF clone of Human bleomycin hydrolase (BLMH), mGFP tagged |
CNY 5,890.00 |
|
| RC200393L3 | Lenti ORF clone of Human bleomycin hydrolase (BLMH), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC200393L4 | Lenti ORF clone of Human bleomycin hydrolase (BLMH), mGFP tagged |
CNY 5,890.00 |
|
| SC320216 | BLMH (untagged)-Human bleomycin hydrolase (BLMH) |
CNY 3,656.00 |
