NARS1 (NM_004539) Human Tagged ORF Clone
CAT#: RG200087
- TrueORF®
NARS (tGFP-tagged) - Human asparaginyl-tRNA synthetase (NARS)
ORF Plasmid: DDK
"NM_004539" in other vectors (4)
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CNY 5,680.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | TurboGFP |
Synonyms | ASNRS; NARS |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RG200087 representing NM_004539
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGTGCTAGCAGAGCTGTACGTCTCTGACCGAGAGGGAAGCGATGCCACGGGAGATGGAACCAAGGAGA AACCATTTAAAACAGGTCTAAAGGCTTTGATGACAGTAGGGAAAGAACCATTTCCTACCATTTACGTAGA TTCACAAAAAGAAAATGAGAGGTGGAATGTTATTTCTAAATCACAGTTGAAGAACATTAAAAAGATGTGG CATAGGGAACAAATGAAGAGTGAATCCCGGGAAAAGAAAGAGGCAGAAGATAGTTTACGAAGAGAAAAGA ACCTGGAAGAAGCAAAGAAGATTACCATTAAAAATGATCCAAGTCTCCCAGAGCCAAAATGTGTGAAGAT TGGTGCGTTAGAAGGATATAGAGGCCAAAGAGTAAAGGTGTTTGGCTGGGTCCACAGGCTGCGCAGGCAA GGAAAGAATTTAATGTTTCTGGTGTTGCGAGATGGTACAGGTTATCTTCAGTGTGTCTTGGCGGATGAGT TGTGTCAGTGCTACAATGGAGTTCTCTTGTCCACGGAGAGCAGTGTTGCAGTGTATGGAATGCTAAATCT TACCCCAAAGGGCAAGCAGGCTCCAGGTGGCCATGAGCTGAGTTGTGACTTCTGGGAACTAATTGGGTTG GCCCCTGCTGGAGGAGCTGACAACCTGATCAATGAGGAGTCTGACGTTGATGTCCAGCTCAACAACAGAC ACATGATGATCCGAGGAGAAAACATGTCCAAAATCCTAAAAGCACGATCCATGGTCACCAGGTGCTTTAG AGATCACTTCTTTGATAGGGGGTACTATGAAGTTACTCCTCCAACATTAGTGCAAACACAAGTAGAAGGT GGTGCCACACTCTTCAAGCTTGACTATTTTGGGGAAGAGGCATTTTTGACTCAATCCTCTCAGTTGTACT TGGAGACCTGCCTCCCAGCCCTGGGAGATGTTTTTTGTATTGCTCAGTCATACCGGGCAGAGCAGTCCAG AACACGAAGGCACCTGGCTGAGTACACTCACGTGGAAGCTGAGTGTCCTTTCCTGACTTTTGACGACCTC CTGAACCGGTTGGAGGACTTGGTTTGTGATGTGGTAGATCGAATATTGAAGTCACCTGCAGGGAGCATAG TGCATGAGCTCAACCCGAACTTTCAGCCCCCCAAACGGCCTTTCAAACGGATGAACTATTCAGATGCTAT CGTTTGGCTAAAAGAACATGATGTAAAGAAAGAAGATGGAACTTTCTATGAATTTGGAGAAGATATCCCA GAAGCTCCTGAGAGACTGATGACAGACACCATTAATGAACCAATCTTGCTGTGTCGATTTCCTGTGGAGA TCAAGTCCTTCTACATGCAGCGATGTCCTGAGGATTCCCGTCTTACTGAATCTGTCGACGTGTTGATGCC CAATGTTGGTGAGATTGTGGGAGGCTCAATGCGTATCTTTGATAGTGAAGAAATACTGGCAGGTTATAAA AGGGAAGGGATTGACCCCACTCCCTATTACTGGTATACGGATCAGAGAAAATACGGTACATGTCCCCATG GAGGATATGGCTTGGGCTTGGAACGATTCTTAACGTGGATTCTGAATAGGTATCACATCCGAGACGTGTG CTTATACCCTCGATTTGTCCAGCGTTGCACGCCA AGCGGACCGACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG200087 representing NM_004539
Red=Cloning site Green=Tags(s) MVLAELYVSDREGSDATGDGTKEKPFKTGLKALMTVGKEPFPTIYVDSQKENERWNVISKSQLKNIKKMW HREQMKSESREKKEAEDSLRREKNLEEAKKITIKNDPSLPEPKCVKIGALEGYRGQRVKVFGWVHRLRRQ GKNLMFLVLRDGTGYLQCVLADELCQCYNGVLLSTESSVAVYGMLNLTPKGKQAPGGHELSCDFWELIGL APAGGADNLINEESDVDVQLNNRHMMIRGENMSKILKARSMVTRCFRDHFFDRGYYEVTPPTLVQTQVEG GATLFKLDYFGEEAFLTQSSQLYLETCLPALGDVFCIAQSYRAEQSRTRRHLAEYTHVEAECPFLTFDDL LNRLEDLVCDVVDRILKSPAGSIVHELNPNFQPPKRPFKRMNYSDAIVWLKEHDVKKEDGTFYEFGEDIP EAPERLMTDTINEPILLCRFPVEIKSFYMQRCPEDSRLTESVDVLMPNVGEIVGGSMRIFDSEEILAGYK REGIDPTPYYWYTDQRKYGTCPHGGYGLGLERFLTWILNRYHIRDVCLYPRFVQRCTP SGPTRTRRLE - GFP Tag - V |
Restriction Sites |
SgfI-RsrII
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_004539 |
ORF Size | 1644 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_004539.4 |
RefSeq Size | 2714 bp |
RefSeq ORF | 1647 bp |
Locus ID | 4677 |
UniProt ID | O43776 |
Domains | tRNA-synt_2, tRNA_anti |
Protein Pathways | Aminoacyl-tRNA biosynthesis |
Gene Summary | Aminoacyl-tRNA synthetases are a class of enzymes that charge tRNAs with their cognate amino acids. Asparaginyl-tRNA synthetase is localized to the cytoplasm and belongs to the class II family of tRNA synthetases. The N-terminal domain represents the signature sequence for the eukaryotic asparaginyl-tRNA synthetases. [provided by RefSeq, Jul 2008] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC200087 | NARS (Myc-DDK-tagged)-Human asparaginyl-tRNA synthetase (NARS) |
CNY 4,080.00 |
|
RC200087L3 | Lenti ORF clone of Human asparaginyl-tRNA synthetase (NARS), Myc-DDK-tagged |
CNY 5,890.00 |
|
RC200087L4 | Lenti ORF clone of Human asparaginyl-tRNA synthetase (NARS), mGFP tagged |
CNY 5,890.00 |
|
SC110958 | NARS (untagged)-Human asparaginyl-tRNA synthetase (NARS) |
CNY 4,088.00 |