RYK (NM_001005861) Human Tagged ORF Clone
CAT#: RC600067
- TrueORF®
RYK (DDK-His-tagged)-Extra Cellular Domain Clone of Homo sapiens receptor-like tyrosine kinase, transcript variant 1, Signal peptide (1-25) plus EC domain (26-224)
"NM_001005861" in other vectors (7)
Need custom modification / cloning service?
Get a free quote
CNY 2,400.00
CNY 3,990.00
Product images
CNY 300.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | DDK-His |
Synonyms | D3S3195; JTK5; JTK5A; RYK1 |
Vector | pCMV6-XL5-DDK-His |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | None |
Sequence Data |
>RC600067 representing leader sequence plus the extracellular domain region of NM_001005861
Red=Cloning site Blue=ORF Green=Tags(s) GTAATACGACTCACTATAGGGCGGCCGCGAATTCGTCGACTGGATCTGGTACCGAGGAGATCCGCCGCCG CGATCGCC ATGCGTGGGGCGGCGCGGCTGGGGCGGCCGGGCCGGAGTTGCCTCCCGGGGGCCCGCGGCCTGAGGGCCC CGCCGCCGCCGCCGCTGCTGCTTCTGCTTGCGCTGTTGCCGCTGCTGCCCGCGCCTGGCGCTGCCGCCGC CCCCGCCCCGCGGCCCCCGGAGCTGCAGTCGGCTTCCGCGGGGCCCAGCGTGAGTCTCTACCTGAGCGAG GACGAGGTGCGCCGGCTGATCGGTCTTGATGCAGAACTTTATTATGTGAGAAATGACCTTATTAGTCACT ACGCTCTATCCTTTAGTCTGTTAGTACCCAGTGAGACAAATTTCCTGCACTTCACCTGGCATGCGAAGTC CAAGGTTGAATATAAGCTGGGATTCCAAGTGGACAATGTTTTGGCAATGGATATGCCCCAGGTCAACATT TCTGTTCAGGGGGAAGTTCCACGCACTTTATCAGTGTTTCGGGTAGAGCTTTCCTGTACTGGCAAAGTAG ATTCTGAAGTTATGATACTAATGCAGCTCAACTTGACAGTAAATTCTTCAAAAAATTTTACCGTCTTAAA TTTTAAACGAAGGAAAATGTGCTACAAAAAACTTGAAGAAGTAAAAACTTCAGCCTTGGACAAAAACACT AGCAGAACTATTTATGATCCTGTACATGCAGCTCCAACCACTACGCGT AGCGGACCGACGCGTTCAGGCGACTACAAGGATGACGACGATAAGGGATCTCATCATCACCATCACCATT AATGAGATCTGGTACCGATATCAAGCTTGTCGACTCTAGA >RC600067 representing signal peptide plus the extracellular domain region of NM_001005861
Red=Cloning sites Green= DDK and 6XHIS Tags MRGAARLGRPGRSCLPGARGLRAPPPPPLLLLLALLPLLPAPGAAAAPAPRPPELQSASAGPSVSLYLSE DEVRRLIGLDAELYYVRNDLISHYALSFSLLVPSETNFLHFTWHAKSKVEYKLGFQVDNVLAMDMPQVNI SVQGEVPRTLSVFRVELSCTGKVDSEVMILMQLNLTVNSSKNFTVLNFKRRKMCYKKLEEVKTSALDKNT SRTIYDPVHAAPTTTR SGPTRTRSGDYKDDDDKGSHHHHHH |
Chromatograms |
CHROMATOGRAMS
Sequencher program is needed, download here. |
Restriction Sites | SgfI-RsrII Cloning Scheme for this gene |
ACCN | NM_001005861 |
ORF Size | 678 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the extra cellular domain of the protein with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001005861.2, NP_001005861.1 |
RefSeq Size | 2942 bp |
RefSeq ORF | 1833 bp |
Locus ID | 6259 |
UniProt ID | P34925 |
Protein Families | Druggable Genome, Protein Kinase, Transmembrane |
MW | 24.9 kDa |
Gene Summary | The protein encoded by this gene is an atypical member of the family of growth factor receptor protein tyrosine kinases, differing from other members at a number of conserved residues in the activation and nucleotide binding domains. This gene product belongs to a subfamily whose members do not appear to be regulated by phosphorylation in the activation segment. It has been suggested that mediation of biological activity by recruitment of a signaling-competent auxiliary protein may occur through an as yet uncharacterized mechanism. The encoded protein has a leucine-rich extracellular domain with a WIF-type Wnt binding region, a single transmembrane domain, and an intracellular tyrosine kinase domain. This protein is involved in stimulating Wnt signaling pathways such as the regulation of axon pathfinding. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Feb 2012] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC215449 | RYK (Myc-DDK-tagged)-Human RYK receptor-like tyrosine kinase (RYK), transcript variant 1 |
CNY 4,544.00 |
|
RC215449L1 | Lenti ORF clone of Human RYK receptor-like tyrosine kinase (RYK), transcript variant 1, Myc-DDK-tagged |
CNY 6,944.00 |
|
RC215449L2 | Lenti ORF clone of Human RYK receptor-like tyrosine kinase (RYK), transcript variant 1, mGFP tagged |
CNY 7,320.00 |
|
RC215449L3 | Lenti ORF clone of Human RYK receptor-like tyrosine kinase (RYK), transcript variant 1, Myc-DDK-tagged |
CNY 7,320.00 |
|
RC215449L4 | Lenti ORF clone of Human RYK receptor-like tyrosine kinase (RYK), transcript variant 1, mGFP tagged |
CNY 7,320.00 |
|
RG215449 | RYK (tGFP-tagged) - Human RYK receptor-like tyrosine kinase (RYK), transcript variant 1 |
CNY 5,990.00 |
|
SC301044 | RYK (untagged)-Human RYK receptor-like tyrosine kinase (RYK), transcript variant 1 |
CNY 4,552.00 |