KIF20B (NM_001284259) Human Tagged ORF Clone
CAT#: RC240214
- TrueORF®
KIF20B (myc-DDK-tagged) - Human kinesin family member 20B (KIF20B), transcript variant 1
ORF Plasmid: tGFP
"NM_001284259" in other vectors (2)
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CNY 18,150.00
CNY 300.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | CT90; KRMP1; MPHOSPH1; MPP-1; MPP1 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC240214 representing NM_001284259
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAATCTAATTTTAATCAAGAGGGAGTACCTCGACCATCTTATGTTTTTAGTGCTGACCCAATTGCAA GGCCTTCAGAAATAAATTTCGATGGCATTAAGCTTGATCTGTCTCATGAATTTTCCTTAGTTGCTCCAAA TACTGAGGCAAACAGTTTCGAATCTAAAGATTATCTCCAGGTTTGTCTTCGAATAAGACCATTTACACAG TCAGAAAAAGAACTTGAGTCTGAGGGCTGTGTGCATATTCTGGATTCACAGACTGTTGTGCTGAAAGAGC CTCAATGCATCCTTGGTCGGTTAAGTGAAAAAAGCTCAGGGCAGATGGCACAGAAATTCAGTTTTTCCAA GGTTTTTGGCCCAGCAACTACACAGAAGGAATTCTTTCAGGGTTGCATTATGCAACCAGTAAAAGACCTC TTGAAAGGACAGAGTCGTCTGATTTTTACTTACGGGCTAACCAATTCAGGAAAAACATATACATTTCAAG GGACAGAAGAAAATATTGGCATTCTGCCTCGAACTTTGAATGTATTATTTGATAGTCTTCAAGAAAGACT GTATACAAAGATGAACCTTAAACCACATAGATCCAGAGAATACTTAAGGTTATCATCAGAACAAGAGAAA GAAGAAATTGCTAGCAAAAGTGCATTGCTTCGGCAAATTAAAGAGGTTACTGTGCATAATGATAGTGATG ATACTCTTTATGGAAGTTTAACTAACTCTTTGAATATCTCAGAGTTTGAAGAATCCATAAAAGATTATGA ACAAGCCAACTTGAATATGGCTAATAGTATAAAATTTTCTGTGTGGGTTTCTTTCTTTGAAATTTACAAT GAATATATTTATGACTTATTTGTTCCTGTATCATCTAAATTCCAAAAGAGAAAGATGCTGCGCCTTTCCC AAGACGTAAAGGGCTATTCTTTTATAAAAGATCTACAATGGATTCAAGTATCTGATTCCAAAGAAGCCTA TAGACTTTTAAAACTAGGAATAAAGCACCAGAGTGTTGCCTTCACAAAATTGAATAATGCTTCCAGTAGA AGTCACAGCATATTCACTGTTAAAATATTACAGATTGAAGATTCTGAAATGTCTCGTGTAATTCGAGTCA GTGAATTATCTTTATGTGATCTTGCTGGTTCAGAACGAACTATGAAGACACAGAATGAAGGTGAAAGGTT AAGAGAGACTGGGAATATCAACACTTCTTTATTGACTCTGGGAAAGTGTATTAACGTCTTGAAGAATAGT GAAAAGTCAAAGTTTCAACAGCATGTGCCTTTCCGGGAAAGTAAACTGACTCACTATTTTCAAAGTTTTT TTAATGGTAAAGGGAAAATTTGTATGATTGTCAATATCAGCCAATGTTATTTAGCCTATGATGAAACACT CAATGTATTGAAGTTCTCCGCCATTGCACAAAAAGTTTGTGTCCCAGACACTTTAAATTCCTCTCAAGAG AAATTATTTGGACCTGTCAAATCTTCTCAAGATGTATCACTAGACAGTAATTCAAACAGTAAAATATTAA ATGTAAAAAGAGCCACCATTTCATGGGAAAATAGTCTAGAAGATTTGATGGAAGACGAGGATTTGGTTGA GGAGCTAGAAAACGCTGAAGAAACTCAAAATGTGGAAACTAAACTTCTTGATGAAGATCTAGATAAAACA TTAGAGGAAAATAAGGCTTTCATTAGCCACGAGGAGAAAAGAAAACTGTTGGACTTAATAGAAGACTTGA AAAAAAAACTGATAAATGAAAAAAAGGAAAAATTAACCTTGGAATTTAAAATTCGAGAAGAAGTTACACA GGAGTTTACTCAGTATTGGGCTCAACGGGAAGCTGACTTTAAGGAGACTCTGCTTCAAGAACGAGAGATA TTAGAAGAAAATGCTGAACGTCGTTTGGCTATCTTCAAGGATTTGGTTGGTAAATGTGACACTCGAGAAG AAGCAGCGAAAGACATTTGTGCCACAAAAGTTGAAACTGAAGAAACACATAATTATGTAGGATTTGAAGA TATTATTGATTCTCTTCAAGATAATGTTGCTGATATTAAGAAACAGGCTGAAATTGCTCACTTATATATT GCATCTCTTCCTGACCCCCAGGAAGCTACTGCTTGTTTAGAACTAAAGTTTAATCAAATTAAAGCTGAAT TAGCTAAAACCAAAGGAGAATTAATCAAAACCAAAGAAGAGTTAAAAAAGAGAGAAAATGAATCAGATTC ATTGATTCAAGAGCTTGAGACATCTAATAAGAAAATAATTACACAGAATCAAAGAATTAAAGAATTGATA AATATAATTGATCAAAAAGAAGATACTATCAACGAATTTCAGAACCTAAAGTCTCATATGGAAAACACAT TTAAATGCAATGACAAGGCTGATACATCTTCTTTAATAATAAACAATAAATTGATTTGTAATGAAACAGT TGAAGTACCTAAGGACAGCAAATCTAAAATCTGTTCAGAAAGAAAAAGAGTAAATGAAAATGAACTTCAG CAAGATGAACCACCAGCAAAGAAAGGGTCTATCCATGTTAGTTCAGCTATCACTGAAGACCAAAAGAAAA GTGAAGAAGTGCGACCGAACATTGCAGAAATTGAAGACATCAGAGTTTTACAAGAAAATAATGAAGGACT GAGAGCATTTTTACTCACTATTGAGAATGAACTTAAAAATGAAAAGGAAGAAAAAGCAGAATTAAATAAA CAGATTGTTCATTTTCAGCAGGAACTTTCTCTTTCTGAAAAAAAGAATTTAACTTTAAGTAAAGAGGTCC AACAAATTCAGTCAAATTATGATATTGCAATTGCTGAATTACATGTGCAGAAAAGTAAAAATCAAGAACA GGAGGAAAAGATCATGAAATTGTCAAATGAGATAGAAACTGCTACAAGAAGCATTACAAATAATGTTTCA CAAATAAAATTAATGCACACGAAAATAGACGAACTACGTACTCTTGATTCAGTTTCTCAGATTTCAAACA TAGATTTGCTCAATCTCAGGGATCTGTCAAATGGTTCTGAGGAGGATAATTTGCCAAATACACAGTTAGA CCTTTTAGGTAATGATTATTTGGTAAGTAAGCAAGTTAAAGAATATCGAATTCAAGAACCCAATAGGGAA AATTCTTTCCACTCTAGTATTGAAGCTATTTGGGAAGAATGTAAAGAGATTGTGAAGGCCTCTTCCAAAA AAAGTCATCAGATTGAGGAACTGGAACAACAAATTGAAAAATTGCAGGCAGAAGTAAAAGGCTATAAGGA TGAAAACAATAGACTAAAGGAGAAGGAGCATAAAAACCAAGATGACCTACTAAAAGAAAAAGAAACTCTT ATACAGCAGCTGAAAGAAGAATTGCAAGAAAAAAATGTTACTCTTGATGTTCAAATACAGCATGTAGTTG AAGGAAAGAGAGCGCTTTCAGAACTTACACAAGGTGTTACTTGCTATAAGGCAAAAATAAAGGAACTTGA AACAATTTTAGAGACTCAGAAAGTTGAATGTAGTCATTCAGCCAAGTTAGAACAAGACATTTTGGAAAAG GAATCTATCATCTTAAAGCTAGAAAGAAATTTGAAGGAATTTCAAGAACATCTTCAGGATTCTGTCAAAA ACACCAAAGATTTAAATGTAAAGGAACTCAAGCTGAAAGAAGAAATCACACAGTTAACAAATAATTTGCA AGATATGAAACATTTACTTCAATTAAAAGAAGAAGAAGAAGAAACCAACAGGCAAGAAACAGAAAAATTG AAAGAGGAACTCTCTGCAAGCTCTGCTCGTACCCAGAATCTGAAAGCAGATCTTCAGAGGAAGGAAGAAG ATTATGCTGACCTGAAAGAGAAACTGACTGATGCCAAAAAGCAGATTAAGCAAGTACAGAAAGAGGTATC TGTAATGCGTGATGAGGATAAATTACTGAGGATTAAAATTAATGAACTGGAGAAAAAGAAAAACCAGTGT TCTCAGGAATTAGATATGAAACAGCGAACCATTCAGCAACTCAAGGAGCAGTTAAATAATCAGAAAGTGG AAGAAGCTATACAACAGTATGAGAGAGCATGCAAAGATCTAAATGTTAAAGAGAAAATAATTGAAGACAT GCGAATGACACTAGAAGAACAGGAACAAACTCAGGTAGAACAGGATCAAGTGCTTGAGGCTAAATTAGAG GAAGTTGAAAGGCTGGCCACAGAATTGGAAAAATGGAAGGAAAAATGCAATGATTTGGAAACCAAAAACA ATCAAAGGTCAAATAAAGAACATGAGAACAACACAGATGTGCTTGGAAAGCTCACTAATCTTCAAGATGA GTTACAGGAGTCTGAACAGAAATATAATGCTGATAGAAAGAAATGGTTAGAAGAAAAAATGATGCTTATC ACTCAAGCGAAAGAAGCAGAGAATATACGAAATAAAGAGATGAAAAAATATGCTGAGGACAGGGAGCGTT TTTTTAAGCAACAGAATGAAATGGAAATACTGACAGCCCAGCTGACAGAGAAAGATAGTGACCTTCAAAA GTGGCGAGAAGAACGAGATCAACTGGTTGCAGCTTTAGAAATACAGCTAAAAGCACTGATATCCAGTAAT GTACAGAAAGATAATGAAATTGAACAACTAAAAAGGATCATATCAGAGACTTCTAAAATAGAAACACAAA TCATGGATATCAAGCCCAAACGTATTAGTTCAGCAGATCCTGACAAACTTCAAACTGAACCTCTATCGAC AAGTTTTGAAATTTCCAGAAATAAAATAGAGGATGGATCTGTAGTCCTTGACTCTTGTGAAGTGTCAACA GAAAATGATCAAAGCACTCGATTTCCAAAACCTGAGTTAGAGATTCAATTTACACCTTTACAGCCAAACA AAATGGCAGTGAAACACCCTGGTTGTACCACACCAGTGACAGTTAAGATTCCCAAGGCTCGGAAGAGGAA GAGTAATGAAATGGAGGAGGACTTGGTGAAATGTGAAAATAAGAAGAATGCTACACCCAGAACTAATTTG AAATTTCCTATTTCAGATGATAGAAATTCTTCTGTCAAAAAGGAACAAAAGGTTGCCATACGTCCATCAT CTAAGAAAACATATTCTTTACGGAGTCAGGCATCCATAATTGGTGTAAACCTGGCCACTAAGAAAAAAGA AGGAACACTACAGAAATTTGGAGACTTCTTACAACATTCTCCCTCAATTCTTCAATCAAAAGCAAAGAAG ATAATTGAAACAATGAGCTCTTCAAAGCTCTCAAATGTAGAAGCAAGTAAAGAAAATGTGTCTCAACCAA AACGAGCCAAACGGAAATTATACACAAGTGAAATTTCATCTCCTATTGATATATCAGGCCAAGTGATTTT AATGGACCAGAAAATGAAGGAGAGTGATCACCAGATTATCAAACGACGACTTCGAACAAAAACAGCCAAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC240214 representing NM_001284259
Red=Cloning site Green=Tags(s) MESNFNQEGVPRPSYVFSADPIARPSEINFDGIKLDLSHEFSLVAPNTEANSFESKDYLQVCLRIRPFTQ SEKELESEGCVHILDSQTVVLKEPQCILGRLSEKSSGQMAQKFSFSKVFGPATTQKEFFQGCIMQPVKDL LKGQSRLIFTYGLTNSGKTYTFQGTEENIGILPRTLNVLFDSLQERLYTKMNLKPHRSREYLRLSSEQEK EEIASKSALLRQIKEVTVHNDSDDTLYGSLTNSLNISEFEESIKDYEQANLNMANSIKFSVWVSFFEIYN EYIYDLFVPVSSKFQKRKMLRLSQDVKGYSFIKDLQWIQVSDSKEAYRLLKLGIKHQSVAFTKLNNASSR SHSIFTVKILQIEDSEMSRVIRVSELSLCDLAGSERTMKTQNEGERLRETGNINTSLLTLGKCINVLKNS EKSKFQQHVPFRESKLTHYFQSFFNGKGKICMIVNISQCYLAYDETLNVLKFSAIAQKVCVPDTLNSSQE KLFGPVKSSQDVSLDSNSNSKILNVKRATISWENSLEDLMEDEDLVEELENAEETQNVETKLLDEDLDKT LEENKAFISHEEKRKLLDLIEDLKKKLINEKKEKLTLEFKIREEVTQEFTQYWAQREADFKETLLQEREI LEENAERRLAIFKDLVGKCDTREEAAKDICATKVETEETHNYVGFEDIIDSLQDNVADIKKQAEIAHLYI ASLPDPQEATACLELKFNQIKAELAKTKGELIKTKEELKKRENESDSLIQELETSNKKIITQNQRIKELI NIIDQKEDTINEFQNLKSHMENTFKCNDKADTSSLIINNKLICNETVEVPKDSKSKICSERKRVNENELQ QDEPPAKKGSIHVSSAITEDQKKSEEVRPNIAEIEDIRVLQENNEGLRAFLLTIENELKNEKEEKAELNK QIVHFQQELSLSEKKNLTLSKEVQQIQSNYDIAIAELHVQKSKNQEQEEKIMKLSNEIETATRSITNNVS QIKLMHTKIDELRTLDSVSQISNIDLLNLRDLSNGSEEDNLPNTQLDLLGNDYLVSKQVKEYRIQEPNRE NSFHSSIEAIWEECKEIVKASSKKSHQIEELEQQIEKLQAEVKGYKDENNRLKEKEHKNQDDLLKEKETL IQQLKEELQEKNVTLDVQIQHVVEGKRALSELTQGVTCYKAKIKELETILETQKVECSHSAKLEQDILEK ESIILKLERNLKEFQEHLQDSVKNTKDLNVKELKLKEEITQLTNNLQDMKHLLQLKEEEEETNRQETEKL KEELSASSARTQNLKADLQRKEEDYADLKEKLTDAKKQIKQVQKEVSVMRDEDKLLRIKINELEKKKNQC SQELDMKQRTIQQLKEQLNNQKVEEAIQQYERACKDLNVKEKIIEDMRMTLEEQEQTQVEQDQVLEAKLE EVERLATELEKWKEKCNDLETKNNQRSNKEHENNTDVLGKLTNLQDELQESEQKYNADRKKWLEEKMMLI TQAKEAENIRNKEMKKYAEDRERFFKQQNEMEILTAQLTEKDSDLQKWREERDQLVAALEIQLKALISSN VQKDNEIEQLKRIISETSKIETQIMDIKPKRISSADPDKLQTEPLSTSFEISRNKIEDGSVVLDSCEVST ENDQSTRFPKPELEIQFTPLQPNKMAVKHPGCTTPVTVKIPKARKRKSNEMEEDLVKCENKKNATPRTNL KFPISDDRNSSVKKEQKVAIRPSSKKTYSLRSQASIIGVNLATKKKEGTLQKFGDFLQHSPSILQSKAKK IIETMSSSKLSNVEASKENVSQPKRAKRKLYTSEISSPIDISGQVILMDQKMKESDHQIIKRRLRTKTAK TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_001284259 |
ORF Size | 5460 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001284259.2 |
RefSeq Size | 6459 bp |
RefSeq ORF | 5463 bp |
Locus ID | 9585 |
UniProt ID | Q96Q89 |
Protein Families | Druggable Genome |
MW | 211.1 kDa |
Gene Summary | Plus-end-directed motor enzyme that is required for completion of cytokinesis (PubMed:11470801, PubMed:12740395). Required for proper midbody organization and abscission in polarized cortical stem cells. Plays a role in the regulation of neuronal polarization by mediating the transport of specific cargos. Participates in the mobilization of SHTN1 and in the accumulation of PIP3 in the growth cone of primary hippocampal neurons in a tubulin and actin-dependent manner. In the developing telencephalon, cooperates with SHTN1 to promote both the transition from the multipolar to the bipolar stage and the radial migration of cortical neurons from the ventricular zone toward the superficial layer of the neocortex. Involved in cerebral cortex growth (By similarity). Acts as an oncogene for promoting bladder cancer cells proliferation, apoptosis inhibition and carcinogenic progression (PubMed:17409436).[UniProtKB/Swiss-Prot Function] |
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