SEC24B (NM_001300813) Human Tagged ORF Clone

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Synonyms	SEC24
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC240099 representing NM_001300813 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCGGCCCCCGCCGGGTCCTCTCACCCGGCCGCCAGCGCCCGGATCCCGCCCAAGTTCGGCGGAGCGG CCGTCTCAGGAGCCGCAGCGCCCGCGGGCCCGGGTGCGGGCCCGGCGCCGCACCAGCAGAACGAGACGGG ATTTCACCATGTTGCCCAAGCTAGTCTTGAACTCCTGGACCCAAGCAATCTGCCCGCCTCGGCCTCCCAA ATTGCTGGGAGTACAGGTCCAGCCCAGAATCAAATGCAGGTTCCATCTGGATATGGATTGCATCATCAAA ACTATATTGCTCCCTCAGGACATTACTCTCAAGGACCTGGGAAAATGACCTCATTGCCATTGGATACCCA GTGTGGTGATTACTACTCTGCTCTCTATACAGTACCAACACAAAATGTGACTCCTAACACAGTGAACCAG CAACCAGGAGCACAGCAGTTGTACAGCAGGGGTCCTCCTGCCCCTCATATTGTGGGATCCACTCTAGGAT CTTTCCAAGGTGCTGCATCGTCAGCATCCCATTTGCATACGAGTGCCTCCCAACCATACTCCTCTTTTGT GAATCACTACAATAGTCCAGCCATGTACTCTGCCAGCTCTTCTGTTGCGTCTCAGGGATTTCCCTCTACT TGTGGTCATTATGCTATGTCAACTGTTTCTAATGCCGCGTATCCTAGTGTTTCATATCCCTCTCTGCCTG CTGGTGATACATATGGGCAAATGTTTACCTCACAGAATGCTCCGACTGTTAGGCCAGTTAAAGATAATTC ATTCTCTGGTCAAAATACAGCTATCAGCCATCCATCGCCACTTCCACCTCTACCATCACAACAGCACCAC CAGCAGCAAAGTCTTTCAGGATACAGTACTCTAACGTGGTCATCTCCAGGCCTTCCATCGACTCAAGACA ATCTCATCCGAAACCACACAGGATCCCTGGCTGTAGCGAACAACAACCCAACCATTACTGTTGCAGATTC TTTATCCTGTCCTGTTATGCAAAATGTTCAGCCTCCCAAGTCCAGCCCAGTGGTATCCACTGTTTTATCA GGATCCTCAGGATCCTCATCAACAAGAACACCTCCCACTGCAAATCACCCAGTTGAGCCTGTGACCTCAG TTACACAGCCATCAGAGCTATTACAACAAAAAGGCGTGCAGTATGGTGAATATGTTAATAACCAAGCTAG CTCCGCACCAACTCCCTTGTCATCAACTTCCGATGATGAGGAAGAGGAGGAGGAGGATGAGGAAGCAGGT GTTGACAGCTCTTCTACCACAAGCAGTGCTTCTCCAATGCCCAACAGTTATGATGCCCTGGAAGGAGGCA GTTACCCAGATATGCTTTCTTCATCAGCAAGCAGTCCTGCTCCTGATCCCGCCCCTGAACCTGATCCTGC TTCTGCTCCAGCTCCAGCTTCAGCTCCAGCTCCTGTCGTCCCTCAGCCTTCAAAAATGGCTAAGCCTTTT GGCTATGGCTATCCAACACTTCAGCCTGGTTATCAGAATGCTACAGCACCACTTATTTCTGGAGTACAGC CCAGTAACCCGGTATATTCTGGATTCCAGCAGTATCCTCAATATCCTGGTGTGAACCAGCTATCCTCCAG TATAGGAGGATTGAGTCTTCAGAGTTCTCCACAACCAGAAAGCCTGAGACCTGTAAACCTTACTCAGGAG AGGAATATTTTACCTATGACTCCTGTTTGGGCTCCTGTACCTAACTTGAATGCAGACCTCAAAAAATTAA ACTGTAGCCCAGATTCATTTCGGTGTACTTTGACAAATATTCCACAGACACAGGCTTTACTGAATAAAGC TAAGCTTCCTTTAGGATTGTTGTTACATCCCTTCAGAGACCTAACGCAATTACCAGTGATAACATCAAAT ACCATTGTGAGGTGCCGATCCTGTCGAACGTATATTAACCCCTTTGTATCCTTCATTGATCAACGTAGAT GGAAATGCAATTTGTGCTATAGAGTAAACGATGTTCCTGAAGAATTTATGTATAACCCCCTTACCCGATC TTATGGAGAGCCTCATAAACGACCAGAAGTTCAGAATTCAACTGTGGAGTTCATTGCTTCTTCAGATTAC ATGCTGCGTCCTCCTCAACCTGCAGTTTACTTGTTTGTTTTAGATGTGTCTCATAATGCAGTGGAAGCTG GATATTTGACAATTTTGTGCCAGTCACTCCTAGAAAATCTAGACAAGCTTCCTGGAGATTCACGAACAAG AATAGGATTCATGACCTTTGATAGCACTATTCATTTCTACAATTTACAAGAAGGATTATCACAGCCTCAA ATGTTGATTGTGTCTGATATAGATGATGTTTTTCTACCTACACCGGATAGTTTACTTGTGAATCTATATG AAAGTAAAGAGCTTATAAAAGACTTACTGAATGCATTACCAAACATGTTCACCAATACAAGAGAAACACA CAGTGCCCTTGGTCCTGCACTTCAGGCTGCCTTTAAATTAATGTCTCCAACAGGTGGCCGTGTGTCTGTA TTTCAGACACAGTTACCTTCCTTGGGTGCAGGACTTCTGCAATCCAGAGAAGATCCTAATCAGAGATCAA GTACAAAGGTGGTACAACATCTTGGCCCTGCAACTGATTTTTATAAGAAACTTGCATTAGATTGCTCGGG ACAGCAAACTGCAGTGGATTTGTTCCTTTTAAGTTCACAGTATTCTGATCTTGCTTCTCTAGCTTGCATG TCCAAGTATTCTGCAGGGTGCATCTATTATTATCCATCATTCCACTATACTCACAATCCTTCACAAGCAG AAAAGTTACAAAAAGACCTAAAACGGTATCTCACAAGAAAAATTGGGTTTGAAGCTGTTATGAGAATAAG GTGTACTAAAGGTCTTTCAATGCACACTTTTCACGGTAACTTCTTTGTCCGTTCTACTGATTTGTTATCC CTTGCCAACATCAATCCTGATGCTGGATTTGCGGTGCAGTTGTCAATTGAAGAAAGTTTAACAGATACTT CCTTAGTATGTTTTCAAACAGCCCTATTATATACATCAAGCAAAGGTGAGCGGAGAATTAGAGTACATAC ACTTTGTTTGCCAGTGGTAAGTTCACTAGCAGATGTATATGCGGGAGTGGATGTACAAGCTGCCATCTGC CTTCTGGCAAACATGGCTGTGGATCGGTCCGTTTCATCAAGTCTGTCAGATGCAAGAGATGCCTTAGTGA ATGCTGTAGTGGACTCATTGTCTGCATATGGCTCAACTGTCTCAAATTTACAGCACTCTGCATTGATGGC GCCCAGCTCCCTCAAGTTGTTTCCTCTCTATGTTTTGGCCCTTCTCAAACAGAAAGCATTTAGAACGGGT ACAAGCACACGGCTGGATGATCGTGTATATGCCATGTGTCAGATAAAGTCTCAGCCACTTGTTCATCTAA TGAAAATGATTCATCCCAACTTATACAGGATAGACAGATTGACAGATGAGGGTGCAGTACATGTTAATGA CAGGATTGTACCACAGCCACCTCTTCAAAAATTGTCTGCAGAGAAGCTGACAAGAGAAGGTGCTTTCCTT ATGGACTGTGGCTCTGTTTTTTACATTTGGGTTGGGAAAGGCTGTGACAATAACTTCATAGAGGATGTGC TTGGATATACTAATTTTGCATCAATACCACAGAAAATGACACATCTTCCAGAGCTAGATACACTTTCATC AGAAAGAGCCAGATCCTTCATAACTTGGCTTAGAGACAGCAGACCATTAAGTCCAATCCTTCACATAGTA AAAGATGAGAGTCCTGCCAAAGCAGAATTTTTTCAGCATTTGATTGAAGACCGGACAGAGGCTGCATTTT CTTACTATGAATTTTTGCTTCATGTTCAGCAGCAGATTTGTAAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC240099 representing NM_001300813 Red=Cloning site Green=Tags(s) MSAPAGSSHPAASARIPPKFGGAAVSGAAAPAGPGAGPAPHQQNETGFHHVAQASLELLDPSNLPASASQ IAGSTGPAQNQMQVPSGYGLHHQNYIAPSGHYSQGPGKMTSLPLDTQCGDYYSALYTVPTQNVTPNTVNQ QPGAQQLYSRGPPAPHIVGSTLGSFQGAASSASHLHTSASQPYSSFVNHYNSPAMYSASSSVASQGFPST CGHYAMSTVSNAAYPSVSYPSLPAGDTYGQMFTSQNAPTVRPVKDNSFSGQNTAISHPSPLPPLPSQQHH QQQSLSGYSTLTWSSPGLPSTQDNLIRNHTGSLAVANNNPTITVADSLSCPVMQNVQPPKSSPVVSTVLS GSSGSSSTRTPPTANHPVEPVTSVTQPSELLQQKGVQYGEYVNNQASSAPTPLSSTSDDEEEEEEDEEAG VDSSSTTSSASPMPNSYDALEGGSYPDMLSSSASSPAPDPAPEPDPASAPAPASAPAPVVPQPSKMAKPF GYGYPTLQPGYQNATAPLISGVQPSNPVYSGFQQYPQYPGVNQLSSSIGGLSLQSSPQPESLRPVNLTQE RNILPMTPVWAPVPNLNADLKKLNCSPDSFRCTLTNIPQTQALLNKAKLPLGLLLHPFRDLTQLPVITSN TIVRCRSCRTYINPFVSFIDQRRWKCNLCYRVNDVPEEFMYNPLTRSYGEPHKRPEVQNSTVEFIASSDY MLRPPQPAVYLFVLDVSHNAVEAGYLTILCQSLLENLDKLPGDSRTRIGFMTFDSTIHFYNLQEGLSQPQ MLIVSDIDDVFLPTPDSLLVNLYESKELIKDLLNALPNMFTNTRETHSALGPALQAAFKLMSPTGGRVSV FQTQLPSLGAGLLQSREDPNQRSSTKVVQHLGPATDFYKKLALDCSGQQTAVDLFLLSSQYSDLASLACM SKYSAGCIYYYPSFHYTHNPSQAEKLQKDLKRYLTRKIGFEAVMRIRCTKGLSMHTFHGNFFVRSTDLLS LANINPDAGFAVQLSIEESLTDTSLVCFQTALLYTSSKGERRIRVHTLCLPVVSSLADVYAGVDVQAAIC LLANMAVDRSVSSSLSDARDALVNAVVDSLSAYGSTVSNLQHSALMAPSSLKLFPLYVLALLKQKAFRTG TSTRLDDRVYAMCQIKSQPLVHLMKMIHPNLYRIDRLTDEGAVHVNDRIVPQPPLQKLSAEKLTREGAFL MDCGSVFYIWVGKGCDNNFIEDVLGYTNFASIPQKMTHLPELDTLSSERARSFITWLRDSRPLSPILHIV KDESPAKAEFFQHLIEDRTEAAFSYYEFLLHVQQQICK TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001300813
ORF Size	3894 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001300813.2, NP_001287742.1
RefSeq Size	4799 bp
RefSeq ORF	3897 bp
Locus ID	10427
UniProt ID	O95487
MW	140.9 kDa
Gene Summary	The protein encoded by this gene is a member of the SEC24 subfamily of the SEC23/SEC24 family, which is involved in vesicle trafficking. The encoded protein is thought to be a cargo-binding component of the COPII vesicle, and is thought to be involved in the transport of secretory proteins from the endoplasmic reticulum to the Golgi apparatus. Mutations in this gene have been associated with neural tube defects, and are thought to be a result of a disruption in interactions with the protein encoded by the VANGL planar cell polarity protein 2 (VANGL2) gene. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Dec 2015]

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