PEX1 (NM_001282677) Human Tagged ORF Clone

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Synonyms	HMLR1; PBD1A; PBD1B; ZWS; ZWS1
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC240056 representing NM_001282677 Red=Cloning site Blue=ORF Green=Tags(s) CTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCCGGCGC GCCC ATGTGGGGCAGCGATCGCCTGGCGGGTGCTGGGGGAGGCGGGGCGGCAGTGACTGTGGCCTTCACCAACG CTCGCGACTGCTTCCTCCACCTGCCGCGGCGTCTCGTGGCCCAGCTGCATCTGCTGCAGAATCAAGCTAT AGAAGTGGTCTGGAGTCACCAGCCTGCATTCTTGAGCTGGGTGGAAGGCAGGCATTTTAGTGATCAAGGT GAAAATGTGGCTGAAATTAACAGACAAGTTGGTCAAAAACTTGGACTCTCAAATGGGGGACAGGTATTTC TCAAGCCATGTTCCCATGTGGTATCTTGTCAACAAGTTGAGGTGGAACCCCTCTCAGCAGATGATTGGGA GATACTGGAGCTGCATGCTGTTTCCCTTGAACAACATCTTCTAGATCAAATTCGAATAGTTTTTCCAAAA GCCATTTTTCCTGTTTGGGTTGATCAACAAACGTACATATTTATCCAAATTGTTGCACTAATACCAGCTG CCTCTTATGGAAGGCTGGAAACTGACACCAAACTCCTTATTCAGCCAAAGACACGCCGAGCCAAAGAGAA TACATTTTCAAAAGCTGATGCTGAATATAAAAAACTTCATAGTTATGGAAGAGACCAGAAAGGAATGATG AAAGAACTTCAAACCAAGCAACTTCAGTCAAATACTGTGGGAATCACTGAATCTAATGAAAACGAGTCAG AGATTCCAGTTGACTCATCATCAGTAGCAAGTTTATGGACTATGATAGGAAGCATTTTTTCCTTTCAATC TGAGAAGAAACAAGAGACATCTTGGGGTTTAACTGAAATCAATGCATTCAAAAATATGCAGTCAAAGGTT GTTCCTCTAGACAATATTTTCAGAGTATGCAAATCTCAACCTCCTAGTATATATAACGCGTCAGCAACCT CTGTTTTTCATAAACACTGTGCCATTCATGTATTTCCATGGGACCAGGAATATTTTGATGTAGAGCCCAG CTTTACTGTGACATATGGAAAGCTAGTTAAGCTACTTTCTCCAAAGCAACAGCAAAGTAAAACAAAACAA AATGTGTTATCACCTGAAAAAGAGAAGCAGATGTCAGAGCCACTAGATCAAAAAAAAATTAGGTCAGATC ATAATGAAGAAGATGAGAAGGCCTGTGTGCTACAAGTAGTCTGGAATGGACTTGAAGAATTGAACAATGC CATCAAATATACCAAAAATGTAGAAGTTCTCCATCTTGGGAAAGTCTGGATTCCAGATGACCTGAGGAAG AGACTAAATATAGAAATGCATGCCGTAGTCAGGATAACTCCAGTGGAAGTTACCCCTAAAATTCCAAGAT CTCTAAAGTTACAACCTAGAGAGAATTTACCTAAAGACATAAGTGAAGAAGACATAAAAACTGTATTTTA TTCATGGCTACAGCAGTCTACTACCACCATGCTTCCTTTGGTAATATCAGAGGAAGAATTTATTAAGCTG GAAACTAAAGATGGACTGAAGGAATTTTCTCTGAGTATAGTTCATTCTTGGGAAAAAGAAAAAGATAAAA ATATTTTTCTGTTGAGTCCCAATTTGCTGCAGAAGACTACAATACAAGTCCTTCTAGATCCTATGGTAAA AGAAGAAAACAGTGAGGAAATTGACTTTATTCTTCCTTTTTTAAAGCTGAGCTCTTTGGGAGGAGTGAAT TCCTTAGGCGTATCCTCCTTGGAGCACATCACTCACAGCCTCCTGGGACGCCCTTTGTCTCGGCAGCTGA TGTCTCTTGTTGCAGGACTTAGGAATGGAGCTCTTTTACTCACAGGAGGAAAGGGAAGTGGAAAATCAAC TTTAGCCAAAGCAATCTGTAAAGAAGCATTTGACAAACTGGATGCCCATGTGGAGAGAGTTGACTGTAAA GCTTTACGAGCTTTGAATGATATGATAAAAGAGTTTATCTCCATGGGAAGTTTGGTTGCACTGATTGCCA CAAGTCAGTCTCAGCAATCTCTACATCCTTTACTTGTTTCTGCTCAAGGAGTTCACATATTTCAGTGCGT CCAACACATTCAGCCTCCTAATCAGGAACAAAGATGTGAAATTCTGTGTAATGTAATAAAAAATAAATTG GACTGTGATATAAACAAGTTCACCGATCTTGACCTGCAGCATGTAGCTAAAGAAACTGGCGGGTTTGTGG CTAGAGATTTTACAGTACTTGTGGATCGAGCCATACATTCTCGACTCTCTCGTCAGAGTATATCCACCAG AGAAAAATTAGTTTTAACAACATTGGACTTCCAAAAGGCTCTCCGCGGATTTCTTCCTGCGTCTTTGCGA AGTGTCAACCTGCATAAACCTAGAGACCTGGGTTGGGACAAGATTGGTGGGTTACATGAAGTTAGGCAGA TACTCATGGATACTATCCAGTTACCTGCCAAGTATCCAGAATTATTTGCAAACTTGCCCATACGACAAAG AACAGGAATACTGTTGTATGGTCCGCCTGGAACAGGAAAAACCTTACTAGCTGGGGTAATTGCACGAGAG AGTAGAATGAATTTTATAAGTGTCAAGGGGCCAGAGTTACTCAGCAAATACATTGGAGCAAGTGAACAAG CTGTTCGGGATATTTTTATTAGAGCACAGGCTGCAAAGCCCTGCATTCTTTTCTTTGATGAATTTGAATC CATTGCTCCTCGGCGGGGTCATGATAATACAGGAGTTACAGACCGAGTAGTTAACCAGTTGCTGACTCAG TTGGATGGAGTAGAAGGCTTACAGGGTGTTTATGTATTGGCTGCTACTAGTCGCCCTGACTTGATTGACC CTGCCCTGCTTAGGCCTGGTCGACTAGATAAATGTGTATACTGTCCTCCTCCTGATCAGGTGTCACGTCT TGAAATTTTAAATGTCCTCAGTGACTCTCTACCTCTGGCAGATGATGTTGACCTTCAGCATGTAGCATCA GTAACTGACTCCTTTACTGGAGCTGATCTGAAAGCTTTACTTTACAATGCCCAATTGGAGGCCTTACATG GAATGCTGCTCTCGAGTGGACTCCAGGATGGAAGTTCCAGCTCTGATAGTGACCTAAGTCTGTCTTCAAT GGTCTTTCTTAACCATAGCAGTGGCTCTGACGATTCAGCTGGAGATGGAGAATGTGGCTTAGATCAGTCC CTTGTTTCTTTAGAGATGTCCGAGATCCTTCCAGATGAATCAAAATTCAATATGTACCGGCTCTACTTTG GAAGCTCTTATGAATCAGAACTTGGAAATGGAACCTCTTCTGATTTGAGCTCACAATGTCTCTCTGCACC AAGCTCCATGACTCAGGATTTGCCTGGAGTTCCTGGGAAAGACCAGTTGTTTTCACAGCCTCCAGTGTTA AGGACAGCTTCACAAGAGGGTTGCCAAGAACTTACACAAGAACAAAGAGATCAACTGAGGGCAGATATCA GTATTATCAAAGGCAGATACCGGAGCCAAAGTGGAGAGGACGAATCCATGAACCAACCAGGACCAATCAA AACCAGACTGGCTATTAGTCAGTCACATTTAATGACTGCACTTGGTCACACAAGACCATCCATTAGTGAA GATGACTGGAAGAATTTTGCTGAGCTATATGAAAGCTTTCAAAATCCAAAGAGGAGAAAAAATCAAAGTG GAACAATGTTTCGACCTGGACAGAAAGTAACTTTAGCA AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC TGGATTACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC240056 representing NM_001282677 Red=Cloning site Green=Tags(s) MWGSDRLAGAGGGGAAVTVAFTNARDCFLHLPRRLVAQLHLLQNQAIEVVWSHQPAFLSWVEGRHFSDQG ENVAEINRQVGQKLGLSNGGQVFLKPCSHVVSCQQVEVEPLSADDWEILELHAVSLEQHLLDQIRIVFPK AIFPVWVDQQTYIFIQIVALIPAASYGRLETDTKLLIQPKTRRAKENTFSKADAEYKKLHSYGRDQKGMM KELQTKQLQSNTVGITESNENESEIPVDSSSVASLWTMIGSIFSFQSEKKQETSWGLTEINAFKNMQSKV VPLDNIFRVCKSQPPSIYNASATSVFHKHCAIHVFPWDQEYFDVEPSFTVTYGKLVKLLSPKQQQSKTKQ NVLSPEKEKQMSEPLDQKKIRSDHNEEDEKACVLQVVWNGLEELNNAIKYTKNVEVLHLGKVWIPDDLRK RLNIEMHAVVRITPVEVTPKIPRSLKLQPRENLPKDISEEDIKTVFYSWLQQSTTTMLPLVISEEEFIKL ETKDGLKEFSLSIVHSWEKEKDKNIFLLSPNLLQKTTIQVLLDPMVKEENSEEIDFILPFLKLSSLGGVN SLGVSSLEHITHSLLGRPLSRQLMSLVAGLRNGALLLTGGKGSGKSTLAKAICKEAFDKLDAHVERVDCK ALRALNDMIKEFISMGSLVALIATSQSQQSLHPLLVSAQGVHIFQCVQHIQPPNQEQRCEILCNVIKNKL DCDINKFTDLDLQHVAKETGGFVARDFTVLVDRAIHSRLSRQSISTREKLVLTTLDFQKALRGFLPASLR SVNLHKPRDLGWDKIGGLHEVRQILMDTIQLPAKYPELFANLPIRQRTGILLYGPPGTGKTLLAGVIARE SRMNFISVKGPELLSKYIGASEQAVRDIFIRAQAAKPCILFFDEFESIAPRRGHDNTGVTDRVVNQLLTQ LDGVEGLQGVYVLAATSRPDLIDPALLRPGRLDKCVYCPPPDQVSRLEILNVLSDSLPLADDVDLQHVAS VTDSFTGADLKALLYNAQLEALHGMLLSSGLQDGSSSSDSDLSLSSMVFLNHSSGSDDSAGDGECGLDQS LVSLEMSEILPDESKFNMYRLYFGSSYESELGNGTSSDLSSQCLSAPSSMTQDLPGVPGKDQLFSQPPVL RTASQEGCQELTQEQRDQLRADISIIKGRYRSQSGEDESMNQPGPIKTRLAISQSHLMTALGHTRPSISE DDWKNFAELYESFQNPKRRKNQSGTMFRPGQKVTLA SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	AscI-RsrII      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001282677
ORF Size	3678 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001282677.2
RefSeq Size	4221 bp
RefSeq ORF	3681 bp
Locus ID	5189
UniProt ID	O43933
Protein Families	Druggable Genome
MW	137 kDa
Gene Summary	This gene encodes a member of the AAA ATPase family, a large group of ATPases associated with diverse cellular activities. This protein is cytoplasmic but is often anchored to a peroxisomal membrane where it forms a heteromeric complex and plays a role in the import of proteins into peroxisomes and peroxisome biogenesis. Mutations in this gene have been associated with complementation group 1 peroxisomal disorders such as neonatal adrenoleukodystrophy, infantile Refsum disease, and Zellweger syndrome. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Sep 2013]

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