NUT (NUTM1) (NM_001284292) Human Tagged ORF Clone

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Synonyms	C15orf55; FAM22H; NUT
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC240011 representing NM_001284292 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGTGGTTACTCTGGGTCCTGGACCTGACTGCCTCATTCTGGAGGCTTCCAGACAGCCACAGTTAGTGC CCAAACCTGAGAGGATGGCTTCAGATGGAGCATCTGCATTGCCGGGACCGGATATGAGCATGAAACCTAG TGCCGCCCTGTCTCCATCCCCTGCACTTCCCTTTCTCCCACCAACTTCTGACCCACCAGACCACCCACCC AGGGAGCCACCTCCACAGCCCATCATGCCTTCAGTATTCTCTCCAGACAACCCTCTGATGCTCTCTGCTT TCCCCAGCTCACTGTTGGTGACAGGGGACGGGGGCCCTTGCCTCAGTGGGGCTGGGGCTGGCAAGGTCAT TGTCAAAGTCAAGACAGAAGGGGGGTCAGCTGAGCCCTCTCAAACTCAGAACTTTATCCTTACTCAGACT GCCCTCAATTCGACTGCCCCGGGCACTCCCTGTGGAGGCCTTGAGGGTCCTGCACCTCCATTTGTGACAG CATCTAATGTGAAGACCATTCTGCCCTCTAAGGCTGTTGGTGTCAGCCAGGAGGGTCCTCCAGGCCTTCC GCCTCAGCCTCCACCACCAGTTGCTCAACTGGTCCCCATTGTGCCCCTGGAAAAAGCTTGGCCAGGGCCA CATGGGACAACCGGGGAAGGAGGTCCTGTGGCCACTCTATCCAAGCCTTCCCTAGGTGACCGCTCCAAAA TTTCCAAGGACGTTTATGAGAACTTCCGTCAGTGGCAGCGTTACAAAGCCTTGGCCCGGAGGCACCTATC CCAGAGTCCTGACACAGAAGCTCTTTCCTGTTTTCTTATCCCAGTGCTTCGTTCCCTGGCCCGGCTGAAG CCCACTATGACCCTGGAGGAGGGACTGCCATTGGCTGTGCAGGAGTGGGAGCACACCAGCAACTTTGACC GGATGATCTTTTATGAGATGGCAGAAAGGTTCATGGAGTTTGAGGCTGAGGAGATGCAGATTCAGAACAC ACAGCTGATGAATGGGTCTCAGGGCCTGTCTCCTGCAACCCCTTTGAAACTTGATCCTCTAGGGCCCCTG GCCTCTGAGGTTTGCCAGCAGCCAGTGTACATTCCGAAGAAGGCAGCCTCCAAGACACGGGCCCCCCGCC GGCGTCAGCGTAAAGCCCAGAGACCTCCTGCTCCTGAGGCACCCAAGGAGATCCCACCAGAAGCTGTGAA GGAGTATGTTGACATCATGGAATGGCTGGTGGGGACTCACTTGGCCACTGGGGAGTCAGATGGAAAACAA GAGGAAGAAGGGCAGCAGCAGGAGGAGGAAGGGATGTATCCAGATCCAGGTCTCCTGAGCTACATCAATG AGCTGTGTTCTCAGAAGGTCTTTGTCTCCAAGGTGGAGGCTGTCATTCACCCTCAATTTCTGGCAGATCT GCTGTCCCCAGAAAAACAGAGAGATCCCTTGGCCTTAATTGAGGAGCTAGAGCAAGAAGAAGGACTCACT CTTGCCCAGCTGGTCCAGAAGCGACTCATGGCCTTGGAAGAGGAGGAAGATGCAGAGGCGCCTCCAAGTT TCAGTGGCGCTCAGTTGGACTCAAGTCCTTCTGGTTCTGTTGAGGATGAAGATGGGGATGGGCGGCTTCG GCCCTCACCTGGGCTTCAGGGGGCTGGGGGCGCCGCTTGCCTTGGAAAGGTTTCTTCTTCAGGAAAACGG GCAAGAGAAGTGCATGGTGGGCAGGAGCAAGCCCTAGATAGCCCCAGAGGGATGCACAGGGATGGGAACA CTCTGCCATCCCCCAGCAGCTGGGACCTGCAGCCAGAACTTGCAGCTCCACAGGGAACTCCGGGACCCTT GGGTGTGGAGAGGAGAGGGTCTGGGAAGGTTATAAACCAGGTATCTCTACATCAGGATGGCCATCTAGGA GGCGCTGGGCCTCCTGGGCACTGCCTGGTGGCTGATAGGACTTCAGAGGCTCTGCCCCTTTGTTGGCAGG GAGGCTTCCAGCCTGAGAGCACTCCCAGTTTGGATGCTGGACTTGCAGAGCTGGCTCCTCTGCAAGGACA AGGGTTAGAAAAGCAAGTCCTGGGATTGCAGAAAGGACAACAAACAGGGGGTCGTGGAGTGCTTCCTCAA GGGAAGGAGCCTTTAGCAGTGCCCTGGGAAGGCTCTTCAGGAGCCATGTGGGGAGATGACAGAGGTACCC CCATGGCTCAGAGTTATGATCAGAATCCTTCCCCTAGAGCAGCTGGGGAGAGGGACGATGTCTGTCTCAG CCCAGGAGTTTGGCTGAGCAGTGAGATGGATGCTGTAGGCTTGGAGCTGCCTGTACAAATAGAGGAGGTC ATAGAGAGCTTCCAAGTTGAGAAGTGTGTAACTGAGTATCAGGAAGGCTGCCAGGGACTGGGCTCCAGGG GCAACATTTCCCTGGGTCCTGGAGAAACCCTAGTACCTGGGGATACGGAGAGCAGTGTGATTCCCTGTGG AGGCACAGTTGCGGCAGCTGCCCTAGAAAAGAGAAACTATTGCAGCTTGCCAGGACCTTTGAGGGCCAAC AGCCCACCCTTGAGGTCCAAAGAAAATCAAGAACAGAGCTGTGAAACCGTAGGGCATCCCAGTGATCTGT GGGCAGAAGGTTGCTTCCCATTGCTAGAAAGTGGTGATTCCACACTGGGGTCTTCCAAAGAAACCCTTCC ACCCACATGCCAAGGCAATCTCCTTATCATGGGGACTGAGGATGCCTCCTCCTTGCCTGAAGCCAGTCAA GAGGCAGGGAGCAGAGGCAATTCCTTTTCTCCTCTGTTGGAAACCATAGAACCTGTCAACATACTAGATG TTAAAGATGACTGTGGCCTCCAACTAAGGGTCAGCGAGGACACCTGCCCACTGAATGTTCATTCTTATGA CCCCCAAGGAGAAGGCAGGGTGGATCCTGATCTGTCCAAGCCTAAAAACCTTGCTCCTTTACAAGAGAGT CAGGAGTCTTACACAACTGGGACTCCCAAAGCAACATCTTCTCACCAGGGCCTTGGAAGCACTTTGCCTA GAAGGGGAACCAGGAATGCCATAGTTCCGAGAGAAACTTCTGTTAGTAAAACACACAGGTCAGCAGACAG GGCCAAAGGAAAGGAGAAAAAGAAAAAGGAAGCAGAGGAAGAGGATGAGGAACTCTCCAACTTTGCTTAC CTCTTGGCCTCTAAACTTAGCCTCTCACCAAGGGAGCATCCCCTCAGTCCTCACCATGCCTCAGGAGGTC AGGGCAGCCAGAGAGCATCCCACCTGCTCCCTGCTGGAGCAAAAGGCCCCAGCAAACTTCCATATCCTGT TGCCAAGTCTGGGAAGCGAGCTCTAGCTGGAGGTCCAGCCCCTACTGAAAAGACACCCCACTCAGGAGCT CAACTTGGGGTCCCCAGGGAGAAACCCCTAGCTCTGGGAGTAGTTCGACCCTCACAGCCTCGTAAAAGGC GGTGTGACAGTTTTGTCACGGGCAGAAGGAAGAAACGACGTCGTAGCCAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC240011 representing NM_001284292 Red=Cloning site Green=Tags(s) MVVTLGPGPDCLILEASRQPQLVPKPERMASDGASALPGPDMSMKPSAALSPSPALPFLPPTSDPPDHPP REPPPQPIMPSVFSPDNPLMLSAFPSSLLVTGDGGPCLSGAGAGKVIVKVKTEGGSAEPSQTQNFILTQT ALNSTAPGTPCGGLEGPAPPFVTASNVKTILPSKAVGVSQEGPPGLPPQPPPPVAQLVPIVPLEKAWPGP HGTTGEGGPVATLSKPSLGDRSKISKDVYENFRQWQRYKALARRHLSQSPDTEALSCFLIPVLRSLARLK PTMTLEEGLPLAVQEWEHTSNFDRMIFYEMAERFMEFEAEEMQIQNTQLMNGSQGLSPATPLKLDPLGPL ASEVCQQPVYIPKKAASKTRAPRRRQRKAQRPPAPEAPKEIPPEAVKEYVDIMEWLVGTHLATGESDGKQ EEEGQQQEEEGMYPDPGLLSYINELCSQKVFVSKVEAVIHPQFLADLLSPEKQRDPLALIEELEQEEGLT LAQLVQKRLMALEEEEDAEAPPSFSGAQLDSSPSGSVEDEDGDGRLRPSPGLQGAGGAACLGKVSSSGKR AREVHGGQEQALDSPRGMHRDGNTLPSPSSWDLQPELAAPQGTPGPLGVERRGSGKVINQVSLHQDGHLG GAGPPGHCLVADRTSEALPLCWQGGFQPESTPSLDAGLAELAPLQGQGLEKQVLGLQKGQQTGGRGVLPQ GKEPLAVPWEGSSGAMWGDDRGTPMAQSYDQNPSPRAAGERDDVCLSPGVWLSSEMDAVGLELPVQIEEV IESFQVEKCVTEYQEGCQGLGSRGNISLGPGETLVPGDTESSVIPCGGTVAAAALEKRNYCSLPGPLRAN SPPLRSKENQEQSCETVGHPSDLWAEGCFPLLESGDSTLGSSKETLPPTCQGNLLIMGTEDASSLPEASQ EAGSRGNSFSPLLETIEPVNILDVKDDCGLQLRVSEDTCPLNVHSYDPQGEGRVDPDLSKPKNLAPLQES QESYTTGTPKATSSHQGLGSTLPRRGTRNAIVPRETSVSKTHRSADRAKGKEKKKKEAEEEDEELSNFAY LLASKLSLSPREHPLSPHHASGGQGSQRASHLLPAGAKGPSKLPYPVAKSGKRALAGGPAPTEKTPHSGA QLGVPREKPLALGVVRPSQPRKRRCDSFVTGRRKKRRRSQ TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001284292
ORF Size	3480 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001284292.1, NP_001271221.1
RefSeq Size	4111 bp
RefSeq ORF	3483 bp
Locus ID	256646
UniProt ID	Q86Y26
MW	123.8 kDa
Gene Summary	Plays a role in the regulation of proliferation. Regulates TERT expression by modulating SP1 binding to TERT promoter binding sites.[UniProtKB/Swiss-Prot Function]

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