MCAF1 (ATF7IP) (NM_001286515) Human Tagged ORF Clone

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Synonyms	AM; ATF-IP; MCAF; MCAF1; p621
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC239975 representing NM_001286515 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGACAGTTTAGAAGAACCTCAGAAAAAAGTCTTTAAGGCTCGAAAAACGATGAGAGTGAGTGATCGTC AGCAACTTGAAGCAGTGTACAAGGTCAAAGAAGAACTGTTGAAAACTGATGTCAAGCTGTTAAATGGCAA CCATGAAAATGGAGATTTGGACCCAACCTCACCTTTGGAAAACATGGATTACATTAAAGACAAGGAAGAG GTGAATGGCATTGAAGAGATTTGTTTTGATCCTGAAGGAAGTAAAGCAGAATGGAAGGAAACACCCTGTA TCCTAAGTGTTAATGTAAAAAACAAGCAGGATGATGATTTAAATTGTGAACCTTTGTCTCCCCATAATAT AACTCCAGAACCAGTCTCTAAACTGCCTGCTGAACCAGTTTCTGGTGATCCAGCCCCTGGTGATCTGGAT GCCGGAGATCCAGCCTCCGGAGTACTGGCCTCTGGTGATTCCACCTCTGGTGATCCCACCTCTAGCGAGC CCTCCTCTAGTGATGCTGCCTCTGGTGATGCAACCTCTGGTGATGCCCCTTCTGGTGATGTGTCCCCTGG TGATGCCACCTCTGGTGATGCCACTGCTGATGATCTCTCCTCTGGTGATCCCACCTCTAGTGATCCCATC CCAGGTGAACCGGTCCCTGTTGAACCCATTTCTGGTGATTGTGCCGCTGATGATATAGCCTCTAGTGAAA TAACTTCTGTTGATCTGGCTTCTGGAGCACCAGCTTCCACTGATCCAGCCTCTGATGATCTGGCCTCTGG TGATCTATCCTCTAGTGAACTGGCCTCTGATGATCTGGCCACTGGTGAACTGGCCTCTGATGAGCTGACT TCTGAATCAACCTTTGATCGTACCTTTGAACCAAAGTCTGTACCAGTTTGTGAACCAGTTCCTGAAATTG ACAATATAGAACCAAGTAGCAATAAAGATGATGATTTTCTTGAAAAAAATGGAGCTGATGAAAAATTAGA GCAAATTCAGAGTAAAGACTCATTGGATGAGAAAAATAAAGCTGATAATAATATTGATGCTAATGAAGAA ACTCTAGAAACAGATGATACAACTATTTGTTCAGATCGACCTCCTGAAAATGAAAAGAAGGTAGAGGAAG ATATTATCACAGAGCTTGCTCTTGGAGAAGATGCTATATCTAGCAGTATGGAAATTGACCAAGGTGAAAA GAATGAAGATGAAACTTCTGCAGATCTTGTAGAAACGATTAATGAAAATGTTATTGAAGATAACAAAAGT GAGAATATCTTAGAAAATACAGACTCTATGGAGACAGATGAAATCATTCCTATTTTGGAAAAGCTTGCAC CTTCTGAGGATGAACTTACTTGCTTTTCTAAAACATCTCTCCTTCCAATCGATGAGACAAATCCAGATTT GGAAGAGAAAATGGAAAGTTCTTTTGGTTCACCATCTAAACAAGAAAGTAGTGAGAGTTTGCCAAAAGAA GCCTTTCTGGTCCTCTCTGATGAAGAGGATATTTCGGGTGAAAAAGATGAGTCTGAAGTTATATCGCAAA ATGAAACGTGCTCTCCAGAAGTAGAAAGTAATGAAAAGGACAACAAACCTGAGGAAGAAGAGCAAGTAAT ACATGAAGATGATGAAAGACCTTCTGAGAAAAATGAATTTTCTAGACGAAAACGTTCTAAATCAGAAGAC ATGGACAATGTACAGTCTAAACGTCGTCGATATATGGAAGAAGAATATGAGGCAGAATTTCAAGTAAAGA TTACAGCCAAAGGAGACATTAACCAGAAACTTCAAAAGGTTATACAGTGGTTGCTGGAAGAAAAATTGTG TGCGCTGCAGTGTGCTGTATTTGATAAGACTTTGGCAGAATTGAAAACACGAGTGGAAAAGATTGAATGT AACAAGAGGCATAAAACAGTTCTCACTGAACTACAGGCCAAGATAGCCAGGTTAACCAAACGCTTTGAAG CAGCCAAAGAAGATCTTAAGAAAAGACATGAACATCCACCCAACCCACCAGTATCACCAGGAAAAACTGT AAATGATGTCAACAGCAATAATAACATGTCTTACAGAAATGCAGGCACAGTGAGACAGATGCTGGAGTCC AAAAGAAATGTAAGCGAGAGTGCACCACCATCCTTTCAAACTCCTGTGAATACAGTATCTTCAACCAATC TTGTCACTCCTCCAGCAGTTGTCAGTAGTCAACCTAAATTGCAGACTCCAGTGACTTCGGGTTCCCTCAC AGCAACGTCAGTTCTTCCTGCACCCAATACAGCTACTGTAGTTGCTACTACTCAGGTGCCTAGTGGAAAT CCCCAGCCTACAATCTCTTTACAGCCTTTGCCAGTGATTTTGCATGTACCTGTTGCAGTATCCTCCCAGC CTCAGCTTCTACAGAGCCATCCAGGGACTTTGGTGACTAATCAACCATCTGGCAATGTTGAATTCATTTC TGTGCAAAGCCCACCTACAGTGAGTGGTCTTACCAAAAATCCAGTATCCTTGCCATCCTTGCCAAATCCC ACTAAACCAAACAACGTTCCTTCTGTGCCCAGTCCTAGTATTCAAAGGAACCCTACTGCCAGTGCTGCAC CATTGGGAACAACACTTGCTGTGCAGGCTGTTCCAACAGCACACTCTATTGTACAAGCCACAAGGACTTC TTTACCCACAGTGGGCCCATCAGGACTCTATAGTCCATCAACTAATCGAGGTCCTATACAGATGAAAATT CCAATTTCTGCATTTAGTACTTCGTCTGCTGCAGAACAGAACAGCAATACCACCCCAAGAATTGAAAACC AGACAAACAAAACAATAGATGCTTCTGTCAGTAAGAAAGCAGCTGATAGCACATCACAGTGTGGAAAAGC CACTGGCAGTGATTCAAGTGGTGTCATTGATCTCACAATGGATGATGAAGAGAGTGGAGCTTCACAAGAC CCCAAAAAACTAAATCACACTCCTGTATCAACCATGAGTTCTTCTCAGCCTGTGTCACGACCATTGCAAC CCATACAACCAGCACCGCCTCTTCAACCATCTGGGGTGCCAACAAGTGGACCATCTCAGACCACCATACA CTTACTACCTACAGCTCCAACTACCGTGAATGTAACACATCGTCCAGTAACTCAGGTGACCACAAGACTC CCTGTACCAAGAGCTCCTGCAAACCACCAGGTGGTTTATACAACTCTTCCTGCACCACCAGCTCAGGCTC CCTTGCGAGGAACTGTTATGCAGGCTCCTGCTGTTCGGCAGGTCAATCCCCAAAATAGTAAGAGATTTTT CTTGTATATGGCCCCAAGATACATG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC239975 representing NM_001286515 Red=Cloning site Green=Tags(s) MDSLEEPQKKVFKARKTMRVSDRQQLEAVYKVKEELLKTDVKLLNGNHENGDLDPTSPLENMDYIKDKEE VNGIEEICFDPEGSKAEWKETPCILSVNVKNKQDDDLNCEPLSPHNITPEPVSKLPAEPVSGDPAPGDLD AGDPASGVLASGDSTSGDPTSSEPSSSDAASGDATSGDAPSGDVSPGDATSGDATADDLSSGDPTSSDPI PGEPVPVEPISGDCAADDIASSEITSVDLASGAPASTDPASDDLASGDLSSSELASDDLATGELASDELT SESTFDRTFEPKSVPVCEPVPEIDNIEPSSNKDDDFLEKNGADEKLEQIQSKDSLDEKNKADNNIDANEE TLETDDTTICSDRPPENEKKVEEDIITELALGEDAISSSMEIDQGEKNEDETSADLVETINENVIEDNKS ENILENTDSMETDEIIPILEKLAPSEDELTCFSKTSLLPIDETNPDLEEKMESSFGSPSKQESSESLPKE AFLVLSDEEDISGEKDESEVISQNETCSPEVESNEKDNKPEEEEQVIHEDDERPSEKNEFSRRKRSKSED MDNVQSKRRRYMEEEYEAEFQVKITAKGDINQKLQKVIQWLLEEKLCALQCAVFDKTLAELKTRVEKIEC NKRHKTVLTELQAKIARLTKRFEAAKEDLKKRHEHPPNPPVSPGKTVNDVNSNNNMSYRNAGTVRQMLES KRNVSESAPPSFQTPVNTVSSTNLVTPPAVVSSQPKLQTPVTSGSLTATSVLPAPNTATVVATTQVPSGN PQPTISLQPLPVILHVPVAVSSQPQLLQSHPGTLVTNQPSGNVEFISVQSPPTVSGLTKNPVSLPSLPNP TKPNNVPSVPSPSIQRNPTASAAPLGTTLAVQAVPTAHSIVQATRTSLPTVGPSGLYSPSTNRGPIQMKI PISAFSTSSAAEQNSNTTPRIENQTNKTIDASVSKKAADSTSQCGKATGSDSSGVIDLTMDDEESGASQD PKKLNHTPVSTMSSSQPVSRPLQPIQPAPPLQPSGVPTSGPSQTTIHLLPTAPTTVNVTHRPVTQVTTRL PVPRAPANHQVVYTTLPAPPAQAPLRGTVMQAPAVRQVNPQNSKRFFLYMAPRYM TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001286515
ORF Size	3315 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001286515.2
RefSeq Size	4413 bp
RefSeq ORF	3318 bp
Locus ID	55729
UniProt ID	Q6VMQ6
Protein Families	Transcription Factors
MW	119.1 kDa
Gene Summary	ATF7IP is a multifunctional nuclear protein that associates with heterochromatin. It can act as a transcriptional coactivator or corepressor depending upon its binding partners (summary by Liu et al., 2009 [PubMed 19106100]).[supplied by OMIM, Nov 2010]

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