UBAP2L (NM_001287816) Human Tagged ORF Clone

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Synonyms	NICE-4; NICE4
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC239945 representing NM_001287816 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGATGACATCGGTGGGCACTAACCGAGCCCGGGGAAACTGGGAACAACCTCAAAACCAAAACCAGACAC AGCACAAGCAGCGGCCACAGGCCACTGCAGAACAAATTAGACTTGCACAGATGATTTCGGACCATAATGA TGCTGACTTTGAGGAGAAGGTGAAACAATTGATTGATATTACAGGCAAGAACCAGGATGAATGTGTGATT GCTTTGCATGACTGCAATGGAGATGTCAACAGAGCTATCAATGTTCTTCTGGAAGGAAACCCAGACACGC ATTCCTGGGAGATGGTCGGGAAGAAGAAGGGAGTCTCAGGCCAGAAGGATGGTGGCCAGACGGAATCCAA TGAGGAAGGCAAAGAAAATCGAGACCGGGACAGAGACTATAGTCGGCGACGTGGTGGGCCACCAAGACGG GGGAGAGGTGCCAGCCGTGGACGAGAGTGTATGCATGGGGCTTTATCAAAACCAGCTGTGGTTCGAGGTC AGGAAAATGGATTGGATGGCACCAAGAGTGGAGGGCCTTCTGGAAGAGGAACAGAAAGAGGCAGAAGGGG CCGTGGCCGAGGCAGAGGTGGCTCTGGTAGGCGAGGAGGAAGGTTTTCTGCTCAAGGAATGGGAACCTTT AACCCAGCTGATTATGCAGAGCCAGCCAATACTGATGATAACTATGGCAATAGCAGCGGCAATACGTGGA ACAACACTGGCCACTTTGAACCAGATGATGGGACGAGTGCATGGAGGACTGCAACAGAGGAGTGGGGGAC TGAAGATTGGAATGAAGATCTTTCTGAGACCAAGATCTTCACTGCCTCTAATGTGTCTTCAGTGCCTCTG CCTGCGGAGAATGTGACAATCACTGCTGGTCAGAGAATTGACCTTGCTGTTCTGCTGGGGAAGACACCAT CTACAATGGAGAATGATTCATCTAATCTGGATCCGTCTCAGGCTCCTTCTCTGGCCCAGCCTCTGGTGTT CAGTAATTCGAAGCAGACTGCCATATCACAGCCTGCTTCAGGGAACACATTTTCTCATCACAGTATGGTG AGCATGTTAGGGAAAGGATTTGGTGATGTCGGTGAAGCTAAAGGCGGCAGTACTACAGGCTCCCAGTTCT TGGAGCAATTCAAGACTGCCCAAGCCCTGGCTCAGTTGGCAGCTCAGCATTCTCAGTCTGGAAGCACCAC CACCTCCTCTTGGGACATGGGCTCGACGACACAATCCCCATCACTGGTGCAGTATGATTTGAAGAACCCA AGTGATTCAGCAGTGCACAGCCCCTTTACAAAGCGCCAGGCTTTTACCCCATCTTCAACCATGATGGAGG TGTTCCTTCAGGAGAAGTCACCTGCAGTGGCTACCTCCACAGCTGCACCTCCACCTCCGTCTTCTCCTCT GCCAAGCAAATCCACATCGGCTCCACAGATGTCGCCTGGATCTTCAGACAACCAGTCCTCTAGCCCTCAG CCGGCTCAGCAGAAACTGAAACAGCAGAAGAAAAAAGCCTCCTTGACTTCTAAGATTCCTGCTCTGGCTG TGGAGATGCCTGGCTCAGCAGATATCTCAGGGCTAAACCTGCAGTTTGGGGCATTGCAGTTTGGGTCAGA GCCTGTCCTTTCTGATTATGAGTCCACCCCCACCACGAGCGCCTCTTCAAGCCAGGCTCCAAGTAGCCTG TATACCAGCACGGCCAGTGAATCATCCTCTACAATTTCATCTAACCAGAGTCAGGAGTCTGGTTATCAGA GCGGCCCAATTCAGTCGACAACCTATACCTCCCAAAATAATGCTCAGGGCCCTCTTTATGAACAGAGATC CACACAGACTCGGCGGTACCCCAGCTCCATCTCTTCATCACCCCAAAAGGACCTGACTCAGGCAAAGAAT GGCTTCAGTTCTGTGCAGGCCACGCAGTTACAGACCACACAATCTGTTGAAGGTGCTACAGGCTCTGCAG TGAAATCTGATTCACCTTCCACTTCTAGCATCCCCCCTCTCAATGAAACGGTATCTGCAGCTTCCTTACT GACGACAACCAATCAGCATTCATCCTCCTTGGGTGGCTTGAGCCACAGTGAGGAGATTCCAAATACTACC ACCACACAACACAGCAGCACGTTATCTACGCAGCAGAATACCCTTTCATCATCAACATCTTCTGGGCGCA CTTCGACATCCACTCTTTTGCACACAAGTGTGGAGAGTGAGGCGAATCTCCATTCTTCCTCCAGCACTTT TTCCACCACATCCAGCACAGTCTCTGCACCTCCCCCAGTGGTCAGTGTCTCCTCCAGTCTCAATAGTGGC AGTAGCCTGGGCCTCAGCCTAGGCAGCAACTCCACTGTCACAGCCTCGACTCGAAGCTCAGTTGCTACGA CTTCAGGAAAAGCTCCTCCCAACCTCCCTCCTGGGGTCCCGCCGTTGTTGCCTAATCCGTATATTATGGC TCCAGGGCTGTTACATGCCTACCCGCCACAAGTATATGGTTATGATGACTTGCAGATGCTTCAGACAAGA TTTCCATTGGATTACTACAGCATCCCATTTCCCACACCCACTACTCCGCTGACTGGGAGGGATGGTAGCC TGGCCAGCAACCCTTATTCTGGTGACCTCACAAAGTTCGGCCGTGGGGATGCCTCCTCCCCAGCCCCGGC CACAACCTTGGCCCAACCCCAACAGAACCAGACGCAGACTCACCATACCACGCAGCAGACATTCCTGAAC CCGGCGCTGCCTCCTGGCTACAGTTACACCAGCCTGCCATACTATACAGGGGTCCCGGGCCTCCCCAGCA CCTTCCAGTATGGGCCTGCTGTGTTCCCTGTGGCTCCTACCTCTTCCAAGCAGCATGGTGTGAATGTCAG TGTGAATGCATCGGCCACCCCTTTCCAACAGCCGAGTGGATATGGGTCTCATGGATACAACACTGGTGTT TCAGTCACCTCCAGTAACACGGGCGTGCCAGATATCTCGGGTTCTGTGTACTCCAAAACCCAGTCCTTTG AGAAACAAGGTTTTCATTCCGGTACTCCTGCTGCTTCCTTCAACTTGCCTTCAGCCCTAGGAAGTGGGGG CCCCATCAATCCGGCCACAGCTGCTGCCTACCCACCTGCCCCCTTTATGCACATTCTGACCCCCCATCAG CAGCCGCATTCTCAGATCCTTCACCATCACCTGCAGCAGGATGGCCAGGACATCCTCAATTTCGTCGATG ACCAGCTTGGTGAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC239945 representing NM_001287816 Red=Cloning site Green=Tags(s) MMTSVGTNRARGNWEQPQNQNQTQHKQRPQATAEQIRLAQMISDHNDADFEEKVKQLIDITGKNQDECVI ALHDCNGDVNRAINVLLEGNPDTHSWEMVGKKKGVSGQKDGGQTESNEEGKENRDRDRDYSRRRGGPPRR GRGASRGRECMHGALSKPAVVRGQENGLDGTKSGGPSGRGTERGRRGRGRGRGGSGRRGGRFSAQGMGTF NPADYAEPANTDDNYGNSSGNTWNNTGHFEPDDGTSAWRTATEEWGTEDWNEDLSETKIFTASNVSSVPL PAENVTITAGQRIDLAVLLGKTPSTMENDSSNLDPSQAPSLAQPLVFSNSKQTAISQPASGNTFSHHSMV SMLGKGFGDVGEAKGGSTTGSQFLEQFKTAQALAQLAAQHSQSGSTTTSSWDMGSTTQSPSLVQYDLKNP SDSAVHSPFTKRQAFTPSSTMMEVFLQEKSPAVATSTAAPPPPSSPLPSKSTSAPQMSPGSSDNQSSSPQ PAQQKLKQQKKKASLTSKIPALAVEMPGSADISGLNLQFGALQFGSEPVLSDYESTPTTSASSSQAPSSL YTSTASESSSTISSNQSQESGYQSGPIQSTTYTSQNNAQGPLYEQRSTQTRRYPSSISSSPQKDLTQAKN GFSSVQATQLQTTQSVEGATGSAVKSDSPSTSSIPPLNETVSAASLLTTTNQHSSSLGGLSHSEEIPNTT TTQHSSTLSTQQNTLSSSTSSGRTSTSTLLHTSVESEANLHSSSSTFSTTSSTVSAPPPVVSVSSSLNSG SSLGLSLGSNSTVTASTRSSVATTSGKAPPNLPPGVPPLLPNPYIMAPGLLHAYPPQVYGYDDLQMLQTR FPLDYYSIPFPTPTTPLTGRDGSLASNPYSGDLTKFGRGDASSPAPATTLAQPQQNQTQTHHTTQQTFLN PALPPGYSYTSLPYYTGVPGLPSTFQYGPAVFPVAPTSSKQHGVNVSVNASATPFQQPSGYGSHGYNTGV SVTSSNTGVPDISGSVYSKTQSFEKQGFHSGTPAASFNLPSALGSGGPINPATAAAYPPAPFMHILTPHQ QPHSQILHHHLQQDGQDILNFVDDQLGE TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001287816
ORF Size	3234 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001287816.1, NP_001274745.1
RefSeq Size	3999 bp
RefSeq ORF	3237 bp
Locus ID	9898
UniProt ID	Q14157
Protein Families	Druggable Genome
MW	113.9 kDa
Gene Summary	Plays an important role in the activity of long-term repopulating hematopoietic stem cells (LT-HSCs).[UniProtKB/Swiss-Prot Function]

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