PCDH1 (NM_001278613) Human Tagged ORF Clone

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Synonyms	PC42; PCDH42
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC239942 representing NM_001278613 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGATTCAGGGATCTGAGTTTCTGGAATCGGGGTTCTGTGCTGGGACTGACTGCTCTTGTGGAGAATC GTGTGTCAGTGGGAGAGGCCCTCCTGATTCTGGGGCCTCCCAGGATGGAGCACCTGAGGCACAGCCCAGG CCCTGGGGGGCAACGGCTACTGCTGCCCTCCATGCTGCTAGCACTGCTGCTCCTGCTGGCTCCATCCCCA GGCCACGCCACTCGGGTAGTGTACAAGGTGCCGGAGGAACAGCCACCCAACACCCTCATTGGGAGCCTCG CAGCCGACTATGGTTTTCCAGATGTGGGGCACCTGTACAAGCTAGAGGTGGGTGCCCCGTACCTTCGCGT GGATGGCAAGACAGGTGACATTTTCACCACCGAGACCTCCATCGACCGTGAGGGGCTCCGTGAATGCCAG AACCAGCTCCCTGGTGATCCCTGCATCCTGGAGTTTGAGGTATCTATCACAGACCTCGTGCAGAATGGCA GCCCCCGGCTGCTAGAGGGCCAGATAGAAGTACAAGACATCAATGACAACACACCCAACTTCGCCTCACC AGTCATCACTCTGGCCATCCCTGAGAACACCAACATCGGCTCACTCTTCCCCATCCCGCTGGCTTCAGAC CGTGATGCTGGTCCCAACGGTGTGGCATCCTATGAGCTGCAGGCTGGGCCTGAGGCCCAGGAGCTATTTG GGCTGCAGGTGGCAGAGGACCAGGAGGAGAAGCAACCACAGCTCATTGTGATGGGCAACCTGGACCGTGA GCGCTGGGACTCCTATGACCTCACCATCAAGGTGCAGGATGGCGGCAGCCCCCCACGCGCCAGCAGTGCC CTGCTGCGTGTCACCGTGCTTGACACCAATGACAACGCCCCCAAGTTTGAGCGGCCCTCCTATGAGGCCG AACTATCTGAGAATAGCCCCATAGGCCACTCGGTCATCCAGGTGAAGGCCAATGACTCAGACCAAGGTGC CAATGCAGAAATCGAATACACATTCCACCAGGCGCCCGAAGTTGTGAGGCGTCTTCTTCGACTGGACAGG AACACTGGACTTATCACTGTTCAGGGCCCGGTGGACCGTGAGGACCTAAGCACCCTGCGCTTCTCAGTGC TTGCTAAGGACCGAGGCACCAACCCCAAGAGTGCCCGTGCCCAGGTGGTTGTGACCGTGAAGGACATGAA TGACAATGCCCCCACCATTGAGATCCGGGGCATAGGGCTAGTGACTCATCAAGATGGGATGGCTAACATC TCAGAGGATGTGGCAGAGGAGACAGCTGTGGCCCTGGTGCAGGTGTCTGACCGAGATGAGGGAGAGAATG CAGCTGTCACCTGTGTGGTGGCAGGTGATGTGCCCTTCCAGCTGCGCCAGGCCAGTGAGACAGGCAGTGA CAGCAAGAAGAAGTATTTCCTGCAGACTACCACCCCGCTAGACTACGAGAAGGTCAAAGACTACACCATT GAGATTGTGGCTGTGGACTCTGGCAACCCCCCACTCTCCAGCACTAACTCCCTCAAGGTGCAGGTGGTGG ACGTCAATGACAACGCACCTGTCTTCACTCAGAGTGTCACTGAGGTCGCCTTCCCGGAAAACAACAAGCC TGGTGAAGTGATTGCTGAGATCACTGCCAGTGATGCTGACTCTGGCTCTAATGCTGAGCTGGTTTACTCT CTGGAGCCTGAGCCGGCTGCTAAGGGCCTCTTCACCATCTCACCCGAGACTGGAGAGATCCAGGTGAAGA CATCTCTGGATCGGGAACAGCGGGAGAGCTATGAGTTGAAGGTGGTGGCAGCTGACCGGGGCAGTCCTAG CCTCCAGGGCACAGCCACTGTCCTTGTCAATGTGCTGGACTGCAATGACAATGACCCCAAATTTATGCTG AGTGGCTACAACTTCTCAGTGATGGAGAACATGCCAGCACTGAGTCCAGTGGGCATGGTGACTGTCATTG ATGGAGACAAGGGGGAGAATGCCCAGGTGCAGCTCTCAGTGGAGCAGGACAACGGTGACTTTGTTATCCA GAATGGCACAGGCACCATCCTATCCAGCCTGAGCTTTGATCGAGAGCAACAAAGCACCTACACCTTCCAG CTGAAGGCAGTGGATGGTGGCGTCCCACCTCGCTCAGCTTACGTTGGTGTCACCATCAATGTGCTGGACG AGAATGACAACGCACCCTATATCACTGCCCCTTCTAACACCTCTCACAAGCTGCTGACCCCCCAGACACG TCTTGGTGAGACGGTCAGCCAGGTGGCAGCCGAGGACTTTGACTCTGGTGTCAATGCTGAGCTGATCTAC AGCATTGCAGGTGGCAACCCTTATGGACTCTTCCAGATTGGGTCACATTCAGGTGCCATCACCCTGGAGA AGGAGATTGAGCGGCGCCACCATGGGCTACACCGCCTGGTGGTGAAGGTCAGTGACCGCGGCAAGCCCCC ACGCTATGGCACAGCCTTGGTCCATCTTTATGTCAATGAGACTCTGGCCAACCGCACGCTGCTGGAGACC CTCCTGGGCCACAGCCTGGACACGCCGCTGGATATTGACATTGCTGGGGATCCAGAATATGAGCGCTCCA AGCAGCGTGGCAACATTCTCTTTGGTGTGGTGGCTGGTGTGGTGGCCGTGGCCTTGCTCATCGCCCTGGC GGTTCTTGTGCGCTACTGCAGACAGCGGGAGGCCAAAAGTGGTTACCAGGCTGGTAAGAAGGAGACCAAG GACCTGTATGCCCCCAAGCCCAGTGGCAAGGCCTCCAAGGGAAACAAAAGCAAAGGCAAGAAGAGCAAGT CCCCAAAGCCCGTGAAGCCAGTGGAGGACGAGGATGAGGCCGGGCTGCAGAAGTCCCTCAAGTTCAACCT GATGAGCGATGCCCCTGGGGACAGTCCCCGCATCCACCTGCCCCTCAACTACCCACCAGGCAGCCCTGAC CTGGGCCGCCACTATCGCTCTAACTCCCCACTGCCTTCCATCCAGCTGCAGCCCCAGTCACCCTCAGCCT CCAAGAAGCACCAGGTGGTACAGGACCTGCCACCTGCAAACACATTCGTGGGCACCGGGGACACCACGTC CACGGGCTCTGAGCAGTACTCCGACTACAGCTACCGCACCAACCCCCCCAAATACCCCAGCAAGCAGGTA GGCCAGCCCTTTCAGCTCAGCACACCCCAGCCCCTACCCCACCCCTACCACGGAGCCATCTGGACCGAGG TGTGGGAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC239942 representing NM_001278613 Red=Cloning site Green=Tags(s) MRFRDLSFWNRGSVLGLTALVENRVSVGEALLILGPPRMEHLRHSPGPGGQRLLLPSMLLALLLLLAPSP GHATRVVYKVPEEQPPNTLIGSLAADYGFPDVGHLYKLEVGAPYLRVDGKTGDIFTTETSIDREGLRECQ NQLPGDPCILEFEVSITDLVQNGSPRLLEGQIEVQDINDNTPNFASPVITLAIPENTNIGSLFPIPLASD RDAGPNGVASYELQAGPEAQELFGLQVAEDQEEKQPQLIVMGNLDRERWDSYDLTIKVQDGGSPPRASSA LLRVTVLDTNDNAPKFERPSYEAELSENSPIGHSVIQVKANDSDQGANAEIEYTFHQAPEVVRRLLRLDR NTGLITVQGPVDREDLSTLRFSVLAKDRGTNPKSARAQVVVTVKDMNDNAPTIEIRGIGLVTHQDGMANI SEDVAEETAVALVQVSDRDEGENAAVTCVVAGDVPFQLRQASETGSDSKKKYFLQTTTPLDYEKVKDYTI EIVAVDSGNPPLSSTNSLKVQVVDVNDNAPVFTQSVTEVAFPENNKPGEVIAEITASDADSGSNAELVYS LEPEPAAKGLFTISPETGEIQVKTSLDREQRESYELKVVAADRGSPSLQGTATVLVNVLDCNDNDPKFML SGYNFSVMENMPALSPVGMVTVIDGDKGENAQVQLSVEQDNGDFVIQNGTGTILSSLSFDREQQSTYTFQ LKAVDGGVPPRSAYVGVTINVLDENDNAPYITAPSNTSHKLLTPQTRLGETVSQVAAEDFDSGVNAELIY SIAGGNPYGLFQIGSHSGAITLEKEIERRHHGLHRLVVKVSDRGKPPRYGTALVHLYVNETLANRTLLET LLGHSLDTPLDIDIAGDPEYERSKQRGNILFGVVAGVVAVALLIALAVLVRYCRQREAKSGYQAGKKETK DLYAPKPSGKASKGNKSKGKKSKSPKPVKPVEDEDEAGLQKSLKFNLMSDAPGDSPRIHLPLNYPPGSPD LGRHYRSNSPLPSIQLQPQSPSASKKHQVVQDLPPANTFVGTGDTTSTGSEQYSDYSYRTNPPKYPSKQV GQPFQLSTPQPLPHPYHGAIWTEVWE TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001278613
ORF Size	3228 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001278613.2
RefSeq Size	3768 bp
RefSeq ORF	3231 bp
Locus ID	5097
UniProt ID	Q08174
Protein Families	Transcription Factors, Transmembrane
MW	117.2 kDa
Gene Summary	This gene belongs to the protocadherin subfamily within the cadherin superfamily. The encoded protein is a membrane protein found at cell-cell boundaries. It is involved in neural cell adhesion, suggesting a possible role in neuronal development. The protein includes an extracelllular region, containing 7 cadherin-like domains, a transmembrane region and a C-terminal cytoplasmic region. Cells expressing the protein showed cell aggregation activity. Alternative splicing occurs in this gene. [provided by RefSeq, Jul 2008]

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