KRBA1 (NM_001290187) Human Tagged ORF Clone

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC239929 representing NM_001290187 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGCGCCAGGTGTCCATCACCTTCAAGGACTTGGCCGTGCGGTTCTCGGAGGAGGAGTGGCGGCTCC TGGAGGAGGGGCAGAGGGAGTTCTACCGAGACGTGATGCGGGAGAACTACGAGACGCTGGTCTCTGTGGG GACAGCTGAGCTGCTCCCCCTCTCTGCTTTCCTGTCACCCTCAGAGCCTGGAAGAGCTGTTGGGGGAGGG AGCCACGCTGATGAGGGGCAGGAGCCTGCTGGTTGTGGAGATCCCCAGGGGGGACAGCCCCGGCACAGCC TGCACCTCACAGCCCTGGTGCAGCTGGTGAAGGAGATCCCAGAGTTCTTGTTTGGAGAAGTCAAGGGCGC TATGGACAGCCCCGAGAGCGAGAGCCGGGGAGCCAGCCTGGATGGAGAGAGAGCGAGCCCCGAGGCTGCG GCAAGAGAGCCCTGCCCTCTCCGAGGCCTGCTCAGCTGCCTTCCAGACGGCCCTACCAGCCAGCCCCACC TGGCCACCACGCCCACCGACAGCTCGTGTTCCAGTGGCCCAACTGGTGACGGGGTCCAGGGAAGTCCTCT CCCCATCAAAACTGCCGACAAACCGTGGCCTACAAGGAAGGAAGGCCCAGGAGCCCTGGGCGGGGAGCCC AGCCCTCCCACCCATAGCCCCAGCAGGAGGAAGAGCCACAGAGGACAGGAGAGAGGGACCTCAGAGGCCG GAATTTCTCCTGGGAACAGCCCCTTGCAAGGCCTCATCAACTGTCTGAAGGAAATCCTTGTGCCTGGGCC CCGGCACCCCGAGACATCCCCAAGCTTCTTGCCACCTCTCCCTAGCCTGGGCACGTCCAGGCTAACCAGA GCAGACCTGGGGCCTGGGAGCCCGCCCTGGGCAGTGAAGACCGAGGCGGTTTCAGGGGATTGTCCCCTCC AGGGTCTGCTACACTGTCTGAAGGAGCTCCCCGAGGCCCAGGACAGGCATCCCAGTCCCTCAGGAGTGGG GAACCGACGGCTACAGGAGAATCCAGGAGCCTGGAAAAGGGGTTCTGGAGGGCCTGGATACCTCCTGACC CCTCCTCCCCATCCTGATCTTGGAGCTGGCGGTCTGCTCTCTGTGAAGATGGAGAACAGCTGGGTCCAGA GCCCCCCAGGACCTGCATCCTGTCAGCCTGGCAGGCAGCCCCTCAGTCCCTCAGCCACTGGAGACACCAG AGGGGTCCCCCAACCCAGCTGGGGCCCTGAGGCTCAAGCTGCCAGTGCCTCAAGCTCACCGCTGGAAGCC CTGGAAGCCTGTCTGAAGGGCATTCCCCCAAATGGGTCGTCACCTTCCCAGCTGCCACCCACTTCTTGTT CCCAGAACCCCCAGCCAGGAGACTCTAGGTCTCAGAAGCCTGAACTGCAACCCCACAGATCACACAGTGA AGAAGCGACCAGAGAGCCTGTTCTGCCTCTGGGTCTGCAGAGCTGTGTGAGAGATGGCCCCAGCAGGCCC CTGGCCCCCCGAGGAACCCCCACCAGCTTCTCCTCATCCAGCAGCACCGACTGGGACCTGGATTTTGGGA GTCCTGTGGGGAACCAGGGGCAGCATCCTGGAAAAGGAAGCCCACCAGGAAGCTCCCCACTGCAGGGTCT GGAGAATTGTCTCAAGGAGATACCTGTGCCTGTGCTGCGGCCTGCCTGGCCCTGCTCCTCAGCAGCAGAC AGGGGACCGAGGAGAGCAGAGCCCAGGAACTGGACAGCAGACAAGGAAGGACTGAGGGCTGAGGCCTGCG AGTCAGCCCGTCTCGGGCAGGGTAGGGGAGAAGCGCCCACCCGGAGCCTCCATCTGGTCAGCCCACAGGT GTTCACCTCCAGCTGCGTCCCCGCCTGCCACCAGCGGGGGTTCAAAGACCCTGGGGCCACCAGGCCAGGA GTGTGGAGGTGGCTCCCAGAGGGGTCTGCCCCCAAGCCCTCCCCGCTGCACTGCCTGGAGAGCGCCCTGA GGGGGATCTTGCCTGTAAGGCCCTTGCGCTTCGCCTGCGTGGGAGGCCCCAGCCCCAGCCCCAGCCCCGG CTCCAGCTCGAGCTTCAGCGGCTCTGAAGGAGAAGACCCGAGGCCAGAGCCTGATCTCTGGAAGCCGCTC CCCCAGGAGAGGGACCGCCTTCCCAGCTGTAAGCCTCCTGTCCCTCTGTCCCCATGTCCTGGTGGGACCC CTGCTGGCAGCAGTGGCGGCAGCCCTGGTGAAGACCCCAGGAGAACAGAGCCCAGGTACTGCAGCGGCCT CGGTGCAGGTACAGCTCAGGATCCCTGCCCGGTTTCTCAGCTGGAGAAAAGGCCCAGGGTTAGTGAAGCA TCCAGAGGCCTGGAGCTTGGACATGGAAGACCCAGAGTTGCAGCCAAGACCCATGAGAGGCTGCTCCCCC AGGGCCCGCCTGAGCTGCCCAGTGAGTCTCCCCCTCCGGAGCTGCCCCCTCCGGAAGCTGCGCCTCCTGT GTTGCCAGCCTCCTCCCTGCAGCCGCCATGCCACTGTGGGAAGCCCCTGCAGCAGGAGCTGCACAGCCTC GGTGCTGCCCTTGCGGAGAAGCTGGATCGGCTCGCCACAGCGCTGGCAGGCCTGGCTCAGGAAGTGGCCA CCATGAGGACCCAGGTGAATCGGCTGGGGAGGCGCCCCCAAGGCCCTGGGCCAATGGGCCAAGCTTCCTG GATGTGGACCCTCCCACGGGGACCTCGCTGGGCTCATGGCCCTGGTCACAGACATCTGCCCTACTGGAGG CAGAAGGGACCCACGAGGCCTAAACCAAAGATCCTGCGTGGCCAGGGAGAGAGCTGCAGGGCTGGTGACC TGCAAGGACTCTCCAGAGGGACCGCTCGCCGGGCACGTCCGCTGCCTCCAGACGCTCCCCCGGCAGAACC TCCTGGGCTCCACTGCAGCTCTTCCCAGCAGCTGCTGTCCTCTACACCCAGCTGCCATGCTGCGCCGCCT GCACACCCCCTCCTCGCACATACCGGGGGCCACCAGAGCCCCCTTCCCCCTTTAGTGCCTGCTGCCTTAC CCCTGCAGGGAGCCTCTCCTCCTGCAGCCAGTGCAGATGCAGACGTGCCGACCTCAGGAGTGGCACCAGA CGGGATCCCAGAGCGGCCCAAGGAGCCGAGCAGCCTGCTGGGAGGAGTGCAGAGGGCCCTCCAGGAAGAA CTGTGGGGTGGGGAGCACAGGGACCCGAGATGGGGGGCGCAT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC239929 representing NM_001290187 Red=Cloning site Green=Tags(s) MARQVSITFKDLAVRFSEEEWRLLEEGQREFYRDVMRENYETLVSVGTAELLPLSAFLSPSEPGRAVGGG SHADEGQEPAGCGDPQGGQPRHSLHLTALVQLVKEIPEFLFGEVKGAMDSPESESRGASLDGERASPEAA AREPCPLRGLLSCLPDGPTSQPHLATTPTDSSCSSGPTGDGVQGSPLPIKTADKPWPTRKEGPGALGGEP SPPTHSPSRRKSHRGQERGTSEAGISPGNSPLQGLINCLKEILVPGPRHPETSPSFLPPLPSLGTSRLTR ADLGPGSPPWAVKTEAVSGDCPLQGLLHCLKELPEAQDRHPSPSGVGNRRLQENPGAWKRGSGGPGYLLT PPPHPDLGAGGLLSVKMENSWVQSPPGPASCQPGRQPLSPSATGDTRGVPQPSWGPEAQAASASSSPLEA LEACLKGIPPNGSSPSQLPPTSCSQNPQPGDSRSQKPELQPHRSHSEEATREPVLPLGLQSCVRDGPSRP LAPRGTPTSFSSSSSTDWDLDFGSPVGNQGQHPGKGSPPGSSPLQGLENCLKEIPVPVLRPAWPCSSAAD RGPRRAEPRNWTADKEGLRAEACESARLGQGRGEAPTRSLHLVSPQVFTSSCVPACHQRGFKDPGATRPG VWRWLPEGSAPKPSPLHCLESALRGILPVRPLRFACVGGPSPSPSPGSSSSFSGSEGEDPRPEPDLWKPL PQERDRLPSCKPPVPLSPCPGGTPAGSSGGSPGEDPRRTEPRYCSGLGAGTAQDPCPVSQLEKRPRVSEA SRGLELGHGRPRVAAKTHERLLPQGPPELPSESPPPELPPPEAAPPVLPASSLQPPCHCGKPLQQELHSL GAALAEKLDRLATALAGLAQEVATMRTQVNRLGRRPQGPGPMGQASWMWTLPRGPRWAHGPGHRHLPYWR QKGPTRPKPKILRGQGESCRAGDLQGLSRGTARRARPLPPDAPPAEPPGLHCSSSQQLLSSTPSCHAAPP AHPLLAHTGGHQSPLPPLVPAALPLQGASPPAASADADVPTSGVAPDGIPERPKEPSSLLGGVQRALQEE LWGGEHRDPRWGAH TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001290187
ORF Size	3192 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001290187.1, NP_001277116.1
RefSeq Size	3786 bp
RefSeq ORF	3195 bp
Locus ID	84626
UniProt ID	A5PL33
MW	112.2 kDa

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