SNX14 (NM_001297614) Human Tagged ORF Clone

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Synonyms	RGS-PX2; SCAR20
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC239802 representing NM_001297614 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGTGCCCTGGGTGCGGACGATGGGGCAGAAGCTGAAGCAGCGGCTGCGACTGGACGTGGGACGCGAGA TCTGCCGCCAGTACCCGCTGTTCTGCTTCCTGCTGCTCTGTCTCAGCGCCGCCTCCCTGCTTCTTAACAG GTATATTCATATTTTAATGATCTTCTGGTCATTTGTTGCTGGAGTTGTCACATTCTACTGCTCACTAGGA CCTGATTCTCTCTTACCAAATATATTCTTCACAATAAAATACAAACCCAAGCAGTTAGGACTTCAGGAAT TATTTCCTCAAGGTCATAGCTGTGCTGTTTGTGGTAAAGTGAAATGTAAACGACATAGGCCTTCTTTGCT ACTTGAAAACTACCAGCCATGGCTAGACCTGAAAATTTCTTCCAAGGTTGATGCATCTCTCTCAGAGGTT CTTGAATTAGTGTTGGAAAACTTTGTTTATCCGTGGTACAGGGATGTGACAGATGATGAATCCTTTGTTG ATGAACTGAGAATAACATTACGTTTTTTTGCATCTGTCTTAATAAGAAGGATTCACAAGGTGGATATTCC ATCTATTATAACCAAGAAACTATTAAAAGCAGCAATGAAGCATATAGAAGTGATAGTTAAAGCCAGACAG AAAGTAAAAAATACAGAGTTTTTACAGCAAGCTGCTTTAGAAGAATATGGTCCAGAGCTTCATGTTGCTT TGAGAAGTCGAAGAGATGAATTGCACTATTTAAGGAAACTTACTGAACTGCTTTTTCCTTATATTTTGCC TCCTAAAGCAACAGACTGCAGATCTCTGACCTTACTTATAAGAGAGATTCTGTCTGGCTCTGTGTTCCTT CCTTCTTTGGATTTCCTAGCTGATCCAGATACTGTGAATCATTTGCTTATCATCTTCATAGATGACAGTC CACCTGAAAAAGCAACTGAACCGGCTTCTCCTTTGGTTCCATTCTTGCAGAAATTTGCAGAACCTAGAAA TAAAAAGCCATCTGTGCTGAAGTTAGAATTGAAGCAAATCAGAGAGCAACAAGATCTTTTATTTCGTTTT ATGAACTTTCTGAAACAAGAAGGCGCAGTGCACGTGTTGCAGTTTTGTTTGACTGTGGAGGAATTTAATG ATAGAATTTTACGACCAGAATTATCAAATGATGAAATGCTGTCTCTTCATGAAGAATTGCAGAAGATTTA TAAAACATACTGTTTGGATGAAAGTATTGACAAAATTAGATTTGATCCCTTCATTGTAGAAGAGATTCAA AGAATTGCTGAAGGCCCATACATAGATGTTGTGAAACTTCAAACTATGAGATGTCTTTTTGAAGCATATG AACATGTTCTTTCCCTTTTGGAGAATGTATTTACTCCTATGTTCTGCCATAGTGATGAGTATTTCAGACA ACTTTTAAGAGGTGCAGAATCACCAACACGCAATTCAAAATTGAACAGGAACACACAGAAAAGGGGAGAA TCATTTGGAATCAGCAGAATAGGTAGCAAAATTAAAGGAGTATTCAAAAGTACCACAATGGAGGGAGCTA TGTTGCCTAATTATGGTGTAGCTGAAGGTGAAGATGATTTTATTGAAGAAGGTATTGTTGTAATGGAAGA TGATTCTCCAGTGGAGGCTGTGAGCACACCTAATACTCCCCGAAACCTTGCTGCATGGAAAATTAGCATT CCATATGTAGACTTTTTTGAGGATCCCTCCTCTGAAAGGAAGGAGAAAAAAGAAAGAATTCCTGTGTTTT GTATTGATGTTGAAAGAAATGATAGAAGAGCAGTTGGACACGAGCCTGAACATTGGTCTGTCTATAGAAG ATATCTTGAATTCTATGTACTTGAATCAAAACTAACAGAATTTCATGGTGCATTTCCTGATGCCCAGCTT CCTTCTAAGAGGATCATTGGCCCCAAAAATTATGAATTCTTAAAGTCAAAGAGGGAAGAGTTCCAAGAAT ATCTACAGAAACTTCTGCAGCATCCAGAACTGAGTAATAGTCAACTTCTGGCAGACTTTCTTTCCCCTAA TGGTGGGGAAACACAATTTCTTGATAAGATACTACCAGATGTAAATCTTGGGAAAATTATAAAATCTGTT CCTGGAAAACTAATGAAAGAGAAAGGTCAGCATTTGGAACCTTTTATCATGAATTTCATTAATTCTTGTG AGTCTCCAAAGCCTAAACCAAGTAGACCAGAACTGACCATTCTCAGCCCTACTTCAGAAAACAACAAGAA GCTTTTCAATGATCTGTTTAAAAATAATGCAAACCGTGCTGAAAATACAGAGAGAAAGCAAAATCAGAAT TATTTTATGGAGGTGATGACTGTAGAAGGAGTCTATGATTACCTGATGTATGTAGGACGGGTAGTTTTCC AGGTTCCTGACTGGCTTCATCATCTCTTAATGGGAACTCGAATCCTCTTTAAAAACACCCTGGAAATGTA TACTGATTACTATCTTCAGTGTAAACTAGAACAGCTATTTCAGGAGCACCGTTTGGTCTCACTCATAACA CTTCTCAGAGATGCTATATTCTGTGAAAACACTGAACCTCGCTCTCTCCAAGATAAGCAAAAAGGAGCAA AACAGACTTTTGAAGAAATGATGAATTACATTCCAGATCTGTTAGTCAAGTGTATTGGTGAAGAAACCAA GTATGAAAGCATCAGACTTCTGTTTGATGGCTTACAGCAACCAGTACTCAACAAGCAGCTGACTTATGTT TTATTGGACATTGTGATACAGGAACTGTTTCCAGAGCTCAATAAGGTACAAAAGGAAGTTACCTCTGTGA CATCTTGGATG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC239802 representing NM_001297614 Red=Cloning site Green=Tags(s) MVPWVRTMGQKLKQRLRLDVGREICRQYPLFCFLLLCLSAASLLLNRYIHILMIFWSFVAGVVTFYCSLG PDSLLPNIFFTIKYKPKQLGLQELFPQGHSCAVCGKVKCKRHRPSLLLENYQPWLDLKISSKVDASLSEV LELVLENFVYPWYRDVTDDESFVDELRITLRFFASVLIRRIHKVDIPSIITKKLLKAAMKHIEVIVKARQ KVKNTEFLQQAALEEYGPELHVALRSRRDELHYLRKLTELLFPYILPPKATDCRSLTLLIREILSGSVFL PSLDFLADPDTVNHLLIIFIDDSPPEKATEPASPLVPFLQKFAEPRNKKPSVLKLELKQIREQQDLLFRF MNFLKQEGAVHVLQFCLTVEEFNDRILRPELSNDEMLSLHEELQKIYKTYCLDESIDKIRFDPFIVEEIQ RIAEGPYIDVVKLQTMRCLFEAYEHVLSLLENVFTPMFCHSDEYFRQLLRGAESPTRNSKLNRNTQKRGE SFGISRIGSKIKGVFKSTTMEGAMLPNYGVAEGEDDFIEEGIVVMEDDSPVEAVSTPNTPRNLAAWKISI PYVDFFEDPSSERKEKKERIPVFCIDVERNDRRAVGHEPEHWSVYRRYLEFYVLESKLTEFHGAFPDAQL PSKRIIGPKNYEFLKSKREEFQEYLQKLLQHPELSNSQLLADFLSPNGGETQFLDKILPDVNLGKIIKSV PGKLMKEKGQHLEPFIMNFINSCESPKPKPSRPELTILSPTSENNKKLFNDLFKNNANRAENTERKQNQN YFMEVMTVEGVYDYLMYVGRVVFQVPDWLHHLLMGTRILFKNTLEMYTDYYLQCKLEQLFQEHRLVSLIT LLRDAIFCENTEPRSLQDKQKGAKQTFEEMMNYIPDLLVKCIGEETKYESIRLLFDGLQQPVLNKQLTYV LLDIVIQELFPELNKVQKEVTSVTSWM TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001297614
ORF Size	2811 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001297614.2, NP_001284543.1
RefSeq Size	3738 bp
RefSeq ORF	2814 bp
Locus ID	57231
UniProt ID	Q9Y5W7
Protein Families	Transmembrane
MW	109.6 kDa
Gene Summary	This gene encodes a member of the sorting nexin family. Members of this family have a phox (PX) phosphoinositide binding domain and are involved in intracellular trafficking. The encoded protein also contains a regulator of G protein signaling (RGS) domain. Regulator of G protein signaling family members are regulatory molecules that act as GTPase activating proteins for G alpha subunits of heterotrimeric G proteins. Alternate splicing results in transcript variants encoding distinct isoforms. [provided by RefSeq, Jul 2014]

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